Production of polyhydroxyalkanoates in open, mixed cultures from a waste sludge stream containing high levels of soluble organics, nitrogen and phosphorus

Morgan-Sagastume, Fernando; Karlsson, Anton; Johansson, Peter; Pratt, Steven; Boon, Nico; Lant, Paul; Werker, Alan

doi:10.1016/j.watres.2010.06.043

Cited by 147 publications

(94 citation statements)

References 40 publications

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“…Nonetheless, when using real wastewater the experimental results indicated that PHA production is primarily associated with the VFA fraction of the wastewater (Coats et al, 2007;Bengtsson et al, 2008;Beccari et al, 2009;Albuquerque et al, 2010;Morgan-Sagastume et al, 2010;Jiang et al, 2012;Tamis et al, 2014a;Morgan-Sagastume et al, 2014, 2015Korkakaki et al, 2016). This correlation is based on the fact that the VFA carbon fraction serves as a PHA precursor and efficiently enriches for a PHA accumulating community.…”

Section: Introductionmentioning

confidence: 97%

Survival of the fastest: Selective removal of the side population for enhanced PHA production in a mixed substrate enrichment

Korkakaki

Loosdrecht

Kleerebezem

2016

Bioresource Technology

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Section: Introductionmentioning

confidence: 97%

Survival of the fastest: Selective removal of the side population for enhanced PHA production in a mixed substrate enrichment

Korkakaki

Loosdrecht

Kleerebezem

2016

Bioresource Technology

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“…However, most 24 chemosynthetic surfactants have poor biodegradability, which can cause a negative 25 environmental impact. 1 Bio-surfactants are eco-friendly with excellent surface activity across a wide 2 temperature and pH range. This makes them a desirable replacement of traditional 3 chemical surfactants.…”

Section: Introductionmentioning

confidence: 99%

“…Finally, surface activity and degradation of the 1 bio-surfactants were evaluated and their effects on VFA production were discussed. 2 3 4 WAS was obtained from a secondary sedimentation tank in a sewage treatment 5 plant (Shanghai, China), which applied an Anaerobic/Anoxic/Oxic (A/A/O) process. 6 The WAS was first screened by an 18 mesh sieve and then concentrated by settling for 7 24 h at 4 o C. The characteristics of concentrated sludge are shown in Table 1.…”

Section: Introductionmentioning

confidence: 99%

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Improved volatile fatty acid production during waste activated sludge anaerobic fermentation by different bio-surfactants

Huang

Shen

Liu

et al. 2015

Chemical Engineering Journal

155

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“…By contrast, in the ADF process, an internal limitation (insufficient intracellular components necessary for growth) prevents growth while both an electron acceptor (oxygen) and its corresponding electron donor (PHA) are present during the substrate uptake (2015). By using fermented waste streams rich in biodegradable carbon compounds and nutrients (nitrogen and phosphorous), it is possible to obtain microorganisms with both high storage ability and PHA accumulation capacity (Morgan-Sagastume et al 2010). However, the PHA composition seems to be affected by the nutrient limitation conditions, especially under phosphorus limitation (Wen et al 2010).…”

Section: Pha Production 212mentioning

confidence: 99%

Composition of mixed culture PHA biopolymers and implications for downstream processing

Montano-Herrera¹

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Polyhydroxyalkanoates (PHAs) are biopolymers synthesised and accumulated by most bacteria for carbon and energy storage. They have properties and applications comparable to petrochemical thermoplastics. Although PHAs are produced at high yields using pure biological cultures, the use of mixed cultures can significantly reduce the production costs and make use of waste streams to produce environmentally sustainable materials. In order to produce mixed culture PHAs of relevance for broader industry applications it is necessary to develop the ability to tailor polymers with diversified mechanical properties through understanding and controlling the monomer composition and compositional distribution.Diverse and complex PHA polymer structures have been achieved in mixed microbial cultures using time-based feeding strategies. However, PHA monomer composition and compositional distribution in PHA random and blocky copolymers are sensitive to substrate feeding history, making more complex the prediction of final PHA content and composition.In this sense, a better understanding of the changing cell physiologies that develop in response to different feeding strategies and substrates is necessary to design optimised feeding strategies and process control algorithms for PHA production.This thesis presents for the first time a comprehensive flux characterisation of monomer development during mixed culture PHA accumulation concurrent with biomass growth using Metabolic Flux Analysis (MFA). A dynamic trend in active biomass growth and in polymer composition was observed and was consistent over replicate accumulations. Monomer (3-hydroxybutyrate and 3-hydroxyvalerate) incorporation into poly(3-hydroxybutyate-co-3-hydroxyvalerate) copolymers in a pilot scale production system was evaluated based on published models that describe polymer production during PHA accumulation. However, it was found that these existing models could not describe the fluctuations in the proportion of Additionally, thermal degradation of mixed culture PHAs during melt processing was assessed by Near-Infrared (NIR) spectroscopy coupled to Multivariate Data Analysis. It was shown that, with correct pretreatment, a copolymeric product that was much more stable to extrusion processing than commercially available PHA was produced.Overall, this thesis explores both the fundamental and applied aspects of PHA production by mixed microbial cultures with concurrent active biomass growth. The use of computational iii tools to explore and help understand the underlying metabolic processes was explored.Polymer composition seems to follow a very complex regulation processes which can be described through the incorporation of more detailed reactions in current metabolic models.Furthermore, this thesis gives further insight into the fundamental properties of the materials produced and an assessment of their potential for degradation during processing.iv

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Production of polyhydroxyalkanoates in open, mixed cultures from a waste sludge stream containing high levels of soluble organics, nitrogen and phosphorus

Cited by 147 publications

References 40 publications

Survival of the fastest: Selective removal of the side population for enhanced PHA production in a mixed substrate enrichment

Survival of the fastest: Selective removal of the side population for enhanced PHA production in a mixed substrate enrichment

Improved volatile fatty acid production during waste activated sludge anaerobic fermentation by different bio-surfactants

Composition of mixed culture PHA biopolymers and implications for downstream processing

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