Production of polyhydroxyalkanoates (PHA) by a thermophilic strain of Schlegelella thermodepolymerans from xylose rich substrates

Kouřilová, Xenie; Pernicová, Iva; Sedlář, Karel; Musilová, Jana; Sedláček, Petr; Kalina, Michal; Koller, Martin; Obruča, Stanislav

doi:10.1016/j.biortech.2020.123885

Cited by 64 publications

(26 citation statements)

References 42 publications

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“…The metagenomic DNA fragment encoding the TreM protein exhibits phylogenetic closeness with Thermanaerothrix daxensis , which grows between 50 to 73°C, with optimum growth at 65°C ( 39 ). At the nucleotide level, the metagenomic DNA fragment ( treM gene) showed similarity with a genomic stretch of Schlegelella thermodepolymerans , which has an optimum growth temperature of 50 to 55°C ( 40 ). The high optimal growth temperature of an organism or the high temperature of the source of the metagenome does not imply that the enzymes encoded by the genomic sequences are adapted to work at high temperatures ( 41 ).…”

Section: Discussionmentioning

confidence: 99%

A Novel Trehalose Synthase for the Production of Trehalose and Trehalulose

Agarwal

Singh

2021

Microbiol Spectr

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Section: Discussionmentioning

confidence: 99%

A Novel Trehalose Synthase for the Production of Trehalose and Trehalulose

Agarwal

Singh

2021

Microbiol Spectr

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“…There are still many economic challenges and limitations of the PHA biotechnological production, which are mainly affected by relatively high production costs and the difficulty of the extraction steps when compared to other biopolymers. Nevertheless, many research groups currently investigate novel production approaches and cultivation strategies in order to increase the competitiveness of these highly promising biopolymers [ 39 , 40 , 41 , 42 ]. The study presented here is consistent with this concept as far as the thermophilic (bacterial) PHA producent has been employed, which suggests the potential reduction of sterility requirements.…”

Section: Discussionmentioning

confidence: 99%

Degradation of P(3HB-co-4HB) Films in Simulated Body Fluids

et al. 2022

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A novel model of biodegradable PHA copolymer films preparation was applied to evaluate the biodegradability of various PHA copolymers and to discuss its biomedical applicability. In this study, we illustrate the potential biomaterial degradation rate affectability by manipulation of monomer composition via controlling the biosynthetic strategies. Within the experimental investigation, we have prepared two different copolymers of 3-hydroxybutyrate and 4-hydroxybutyrate—P(3HB-co-36 mol.% 4HB) and P(3HB-co-66 mol.% 4HB), by cultivating the thermophilic bacterial strain Aneurinibacillus sp. H1 and further investigated its degradability in simulated body fluids (SBFs). Both copolymers revealed faster weight reduction in synthetic gastric juice (SGJ) and artificial colonic fluid (ACF) than simple homopolymer P3HB. In addition, degradation mechanisms differed across tested polymers, according to SEM micrographs. While incubated in SGJ, samples were fragmented due to fast hydrolysis sourcing from substantially low pH, which suggest abiotic degradation as the major degradation mechanism. On the contrary, ACF incubation indicated obvious enzymatic hydrolysis. Further, no cytotoxicity of the waste fluids was observed on CaCO-2 cell line. Based on these results in combination with high production flexibility, we suggest P(3HB-co-4HB) copolymers produced by Aneurinibacillus sp. H1 as being very auspicious polymers for intestinal in vivo treatments.

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“…Moreover, no energetically demanding cooling is required. Therefore, processes employing thermophiles can be surprisingly energetically feasible [ 48 ]. Both tested strains are capable of accumulation of substantial amounts of PHA on simple and cheap mineral media, however, R. xylanophilu s seems to be the more flexible strain in terms of a range of utilizable substrates.…”

Section: Discussionmentioning

confidence: 99%

The First Insight into Polyhydroxyalkanoates Accumulation in Multi-Extremophilic Rubrobacter xylanophilus and Rubrobacter spartanus

et al. 2021

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Actinobacteria belonging to the genus Rubrobacter are known for their multi-extremophilic growth conditions—they are highly radiation-resistant, halotolerant, thermotolerant or even thermophilic. This work demonstrates that the members of the genus are capable of accumulating polyhydroxyalkanoates (PHA) since PHA-related genes are widely distributed among Rubrobacter spp. whose complete genome sequences are available in public databases. Interestingly, all Rubrobacter strains possess both class I and class III synthases (PhaC). We have experimentally investigated the PHA accumulation in two thermophilic species, R. xylanophilus and R. spartanus. The PHA content in both strains reached up to 50% of the cell dry mass, both bacteria were able to accumulate PHA consisting of 3-hydroxybutyrate and 3-hydroxyvalerate monomeric units, none other monomers were incorporated into the polymer chain. The capability of PHA accumulation likely contributes to the multi-extremophilic characteristics since it is known that PHA substantially enhances the stress robustness of bacteria. Hence, PHA can be considered as extremolytes enabling adaptation to extreme conditions. Furthermore, due to the high PHA content in biomass, a wide range of utilizable substrates, Gram-stain positivity, and thermophilic features, the Rubrobacter species, in particular Rubrobacter xylanophilus, could be also interesting candidates for industrial production of PHA within the concept of Next-Generation Industrial Biotechnology.

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Production of polyhydroxyalkanoates (PHA) by a thermophilic strain of Schlegelella thermodepolymerans from xylose rich substrates

Cited by 64 publications

References 42 publications

A Novel Trehalose Synthase for the Production of Trehalose and Trehalulose

A Novel Trehalose Synthase for the Production of Trehalose and Trehalulose

Degradation of P(3HB-co-4HB) Films in Simulated Body Fluids

The First Insight into Polyhydroxyalkanoates Accumulation in Multi-Extremophilic Rubrobacter xylanophilus and Rubrobacter spartanus

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