Production of polyhydroxybutyrate by the cyanobacterium cf. Anabaena sp.

Simonazzi, Mara; Pezzolesi, Laura; Galletti, Paola; Gualandi, Chiara; Pistocchi, Rossella; Marco, N.; Paganelli, Zoe; Samorì, Chiara

doi:10.1016/j.ijbiomac.2021.09.054

Cited by 17 publications

(4 citation statements)

References 31 publications

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“…Mixotrophic regime (presence of organic and inorganic carbon sources) with acetate resulted in 46% dcw PHB by Anabaena sp. after 7 days of incubation [13]. Interestingly, under heterotrophic conditions, Synechocistys sp.…”

Section: Introductionmentioning

confidence: 96%

New strategy for bioplastic and exopolysaccharides production: Enrichment of field microbiomes with cyanobacteria

Altamira-Algarra¹,

Rueda²,

Lage³

et al. 2023

Preprint

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Seven photosynthethic microbiomes were collected from field environmental samples to test their potential in polyhydroxybutirate (PHB) and exopolysaccharides (EPS) production, two alternatives to chemical-based polymers. Microscope observations together with microbial sequence analysis revealed the microbiome enrichment in cyanobacteria after culture growth under phosphorus limitation. PHB and EPS production was studied under three culture factors (phototrophy, mixotrophy and heterotrophy) by evaluating and optimizing the effect of three parameters (organic and inorganic carbon and days under light:dark cycles) by Box-Behnken design. Results showed that optimal conditions for both biopolymers synthesis were microbiome-dependent; however, the addition of organic carbon boosted PHB production in all the tested microbiomes, producing up to 14%dcw PHB with the addition of 1.2 g acetate L-1 and seven days under light:dark photoperiods. The highest EPS production was 59 mg L-1 with the addition of 1.2 g acetate L-1 and four days under light:dark photoperiods. The methodology used in this article is suitable for enriching microbiomes in cyanobacteria, and for testing the best conditions for bioproducts synthesis for further scale up.

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Section: Introductionmentioning

confidence: 96%

New strategy for bioplastic and exopolysaccharides production: Enrichment of field microbiomes with cyanobacteria

Altamira-Algarra¹,

Rueda²,

Lage³

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Research on PHA production by bacteria is extensive, but studies involving mixed cultures with cyanobacteria are relatively limited. While results on PHB synthesis by cyanobacteria pure cultures have demonstrated their potential as biopolymer producers [7][8][9][10]13,15,16], current production yields may not yet meet the demands of a market predominantly dominated by petroleum-based plastics. Therefore, efforts to boost their productivity should be pursued.…”

Section: Discussionmentioning

confidence: 99%

“…This has led to PHB production of up to 46 % dcw PHB by cyanobacteria monocultures of Anabaena sp. 11 , 26 % dcw PHB by Synechoccocus sp. 6 and 22 % dcw PHB by Synechocystis sp.…”

Section: Introductionmentioning

confidence: 99%

Bioplastic production by harnessing cyanobacteria-rich microbiomes for perpetual synthesis

Altamira-Algarra,

Lage,

Meléndez

et al. 2023

Preprint

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Departing from the conventional axenic and heterotrophic cultures, our research ventures into unexplored territory by investigating the potential of photosynthetic microbiomes for polyhydroxybutyrate (PHB) production. A cyanobacteria-rich microbiome was harnessed for PHB production in a 3 L photobioreactor under non-sterile conditions. The robust culture achieved up to 28 %dcwPHB over 108 days of alternating growth and accumulation phases. Nile Blue staining and Transmission Electron Microscope visualization successfully confirmed the presence of PHB granules within cyanobacteria cells. Analysis through proton Nuclear Magnetic Resonance further validated the extracted polymer as PHB. In addition, the overexpression of the enzyme PHA synthase throughout the accumulation phase correlated directly with the increased PHB production. Also, gene expression changes suggested that initially, glycogen served as the primary storage compound. However, with prolonged macronutrient stress, there was a shift of the carbon flux towards favoring PHB synthesis. Overall, it was demonstrated for the first time the feasibility of using a phototrophic microbiome to continuous production of PHB in a non-sterile system, contributing to advancing in the field of biopolymer production and offering valuable insights into the metabolic pathways involved.

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“…PHB is relatively brittle compared to PLA, which has better flexibility, however, still lower than traditional polyolefins. The addition of plasticizers can improve the flexibility of both polymers, addressing any brittleness concerns (Kartik et al 2021 ; Kato 2019 ; Simonazzi et al 2021 ). More importantly, PLA, PHA, starch, and protein could also be obtained from microalgae.…”

Section: Swot Analysis: Algal-based Bioplasticsmentioning

confidence: 99%

Algal-based bioplastics: global trends in applied research, technologies, and commercialization

Mogany,

Bhola,

Bux

2024

Environ Sci Pollut Res

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The excessive global demand for plastic materials has resulted in severe plastic waste pollution. Conventional plastics derived from non-renewable fossil fuels are non-biodegradable, leading to significant environmental problems. Algal-based bioplastics represent a more viable, renewable, and sustainable alternative to conventional plastics. They have identical properties and characteristics as conventional plastics while being naturally biodegradable. The potential of the algal biomass value chain has already been well-established by researchers. Here, we review the novel insights on research, technology, and commercialization trends of algal-based bioplastics, encompassing macroalgae and green microalgae/cyanobacteria. Data showed that within the last decade, there has been substantial interest in utilizing microalgae for biopolymer production, with more focus on using cyanobacterial species compared to green algae. Moreover, most of the research conducted has largely focused on the production of PHA or its co-polymers. Since 2011, there have been a total of 55 patents published related to algal-based bioplastics production. To date, ~ 81 entities worldwide (commercial and private businesses) produce bioplastics from algae. Overall results of this study emphasized that even with the economic and social challenges, algae possess a substantial potential for the sustainable development of bioplastics while also addressing the UN’s SDGs.

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Production of polyhydroxybutyrate by the cyanobacterium cf. Anabaena sp.

Cited by 17 publications

References 31 publications

New strategy for bioplastic and exopolysaccharides production: Enrichment of field microbiomes with cyanobacteria

New strategy for bioplastic and exopolysaccharides production: Enrichment of field microbiomes with cyanobacteria

Bioplastic production by harnessing cyanobacteria-rich microbiomes for perpetual synthesis

Algal-based bioplastics: global trends in applied research, technologies, and commercialization

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