Production of polyhydroxybutyrates and carbohydrates in a mixed cyanobacterial culture: Effect of nutrients limitation and photoperiods

Arias, Dulce M.; Uggetti, Enrica; García, Joan

doi:10.1016/j.nbt.2018.01.001

Cited by 54 publications

(28 citation statements)

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“…Concerning the PHB content, results obtained with the wastewater-borne cyanobacteria utilized in this study, for all the conditions, achieved a higher PHB content in shorter time in comparison with pure cultures and even with the same type of culture [47]. Generally, most of the studies showed a very low process of PHB accumulation, reaching percentages lower than 1% in the first 24h.…”

Section: Comparison Of a Mixed Cyanobacterial Culture Enhancement In mentioning

confidence: 53%

Polymer accumulation in mixed cyanobacterial cultures selected under the feast and famine strategy

et al. 2018

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In this study, a sequencing batch reactor (SBR), operated with transient carbon availability (feast and famine) and different nutrients loads, was used to select cyanobacteria accumulating poly (3-hydroxyalkanoate) (PHB) and carbohydrates from a mixed wastewater-borne microbial culture. The SBR was operated with 12h aerobic light and 12h anaerobic dark phases, evaluating the effect of three different operational conditions consisting on; 1) carbon limitation, 2) carbon and phosphorus limitation and 3) phosphorus limitation. Once a steady state was reached in each operational period of the SBR, part of the biomass was collected and submitted to separate batch tests in order to investigate the maximum PHB and carbohydrates accumulation levels. Batch tests were performed during 24h of illuminated aerobic condition and 24h of dark anaerobic condition, while inorganic carbon was constantly present. During the SBR operation, inorganic carbon was mostly used for biomass and carbohydrate production, showing very low PHB accumulation levels (<1%). Notwithstanding, in subsequent batch tests, PHB was accumulated after a complete depletion of nitrogen, reaching almost 4%. Concerning carbohydrates, it was found that phosphorus limitation (with and without carbon limitation) led to a culture mostly dominated by cyanobacteria and higher levels of carbohydrate content (43%-48%) than the culture with carbon limitation and high loads of nitrogen and phosphorus (29%). Such contents were obtained in only 24h of incubation under aerobic illuminated conditions. Hence, these encouraging results indicate that carbon uptake and the consequent polymers production from cyanobacteria can be enhanced through carbon and nutrient feeding strategies.

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Section: Comparison Of a Mixed Cyanobacterial Culture Enhancement In mentioning

confidence: 53%

Polymer accumulation in mixed cyanobacterial cultures selected under the feast and famine strategy

et al. 2018

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“…Theoretical SRT in both photobioreactors was fixed at 10 days. This SRT was chosen based on the results of the previous works (Arias et al, 2017(Arias et al, , 2018a(Arias et al, , 2018b, demonstrating that SRT lower than 10 affects negatively cyanobacterial growth.…”

Section: Experimental Set-upmentioning

confidence: 99%

Selection of cyanobacteria over green algae in a photo-sequencing batch bioreactor fed with wastewater

Arias

Rueda

García-Galán

et al. 2019

Science of The Total Environment

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In this work, a strategy based on photo-sequencing batch operation was used to select cyanobacteria over unsettled green algae in a wastewater treatment system, evaluating for the first time the effect of hydraulic regimes on nutritional dynamics and microorganisms' competition. During 30 days of operation, an initial microalgae mixed consortia dominated by the green microalgae Scenedesmus sp. was cultivated in two different photo-sequencing batch reactors operated at hydraulic retention time (HRT) of 6 days (PSBR6) and 4 days (PSBR4) at a theoretical solids retention time (SRT) of 10 d.Both reactors were compared with a semi-continuous reactor (SC10) operated at 10 d of HRT and 10 days of SRT (used as a control). The results indicated that PSBR6 and PSBR4decreased Scenedesmus sp. population by 88% and 48%, respectively. However, only PSBR6 provided suitable conditions to select cyanobacteria from an initial green algae dominated culture. These conditions included volumetric loads of 11.72 mg TN L −1 d −1 , 2.04 mg TP L −1 d −1 and 53.31 mg TOC L −1 d −1 . The remaining nutrients in the culture led also to a phosphorus limiting N:P ratio (34:1) that improved the increase of cyanobacteria from an initial 2% until 70% of the total population. In addition, PSBR6 reached a biomass production of 0.12 g L -1 d -1 , while removing TN, TP and TOC by 58%, 83% and 85%, respectively. Conversely, the application of higher nutrients loads caused by lower HRT (PSBR4) led to an increase of only 13% of cyanobacteria while SC10 remained with the same biomass composition during all the experimental time. Thus, this study showed that the dominance of cyanobacteria in microalgal-based wastewater treatment systems can be controlled by the operational and nutritional conditions. This knowledge could contribute to control microalgae contamination from up-scaling cyanobacterial biomass production in wastewater treatment systems.

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“…However, the yield of PHB to COD (YPHB/COD) was low, so that it needed nutrient addition to increasing the YPHB/COD. Based on previous research, limiting primary nutrient (N or P) could increase YPHB/COD [17]. Quantitatively, biomass productivity () and YPHB/COD in each reactor was shown in Table 1.…”

Section: Poly-hydroxy Butyrate (Phb) Formationmentioning

confidence: 94%

Vinasse as Cultivation Medium of Chlorella sp. to Produce Poly–Hydroxy Butyrate in Various Limited Low–Cost Primary Nutrient

et al. 2021

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Vinasse is ethanol wastewater that still contains nutrients. It can be medium cultivation for Chlorella sp. Cultivation Chlorella sp. in vinasse did not only minimize its COD content but also potentially produce Poly–Hydroxy Butyrate (PHB) in a limited nutrient. This paper presents a cultivation process of Chlorella sp. in vinasse on the various limited nutrient. Chlorella sp. was cultivated in vinasse by adding complete nutrient (urea and TSP), TSP (limited N), and urea (limited P). Experimental data was optimized by a mathematical model to predict the behavior of the Chlorella sp. in limited nutrient systematically. The study confirmed that the best condition of the cultivation medium of Chlorella sp. to minimize COD content in vinasse by addition phosphate into the reactor. However, PHB could be best accumulated in the cell on limited phosphate.

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Production of polyhydroxybutyrates and carbohydrates in a mixed cyanobacterial culture: Effect of nutrients limitation and photoperiods

Cited by 54 publications

References 50 publications

Polymer accumulation in mixed cyanobacterial cultures selected under the feast and famine strategy

Polymer accumulation in mixed cyanobacterial cultures selected under the feast and famine strategy

Selection of cyanobacteria over green algae in a photo-sequencing batch bioreactor fed with wastewater

Vinasse as Cultivation Medium of Chlorella sp. to Produce Poly–Hydroxy Butyrate in Various Limited Low–Cost Primary Nutrient

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