Production of Potato Cyst Nematode Hatching Stimulus by Hairy Root Cultures of Tomato

Ashikawa, Ikuo; Fukuzawa, Akio; Murai, Akio; Kamada, Hiroshi; Koshi, Masato

doi:10.1080/00021369.1991.10870927

Cited by 1 publication

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References 2 publications

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“…As previously reported by Ashikawa et al (1991), we showed that bacteria-free culture media of the host plant hairy roots can strongly induce the hatching of the PCN eggs, indicating that the HFs are definitely biosynthesized and secreted by the hairy roots. Hairy roots are well used materials in the field of plant secondary metabolism because of its stable production of metabolites.…”

Section: Hs Activity Of Potato and Tomato Hairy Root Culture Mediasupporting

confidence: 84%

Hatching stimulation activity of steroidal glycoalkaloids toward the potato cyst nematode, <i>Globodera rostochiensis</i>

Shimizu

Kushida

Akiyama

et al. 2020

Plant Biotechnology

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Cyst nematodes (Globodera spp. and Heterodera spp.) are highly evolved sedentary endoparasites that are considered as harmful pests worldwide. The hatching of the dormant eggs of cyst nematodes occurs in response to hatching factors (HFs), which are compounds that are secreted from the roots of host plants. Solanoeclepin A (SEA), a triterpene compound, has been isolated as HF for potato cyst nematode (PCN) eggs, whereas other compounds, such as steroidal glycoalkaloids (SGAs), are also known to show weak hatching stimulation (HS) activity. However, the structures of both compounds are different and the HF-mediated hatching mechanism is still largely unknown. In the present study, we observed specific hatching of PCN eggs stimulated by the hairy root culture media of potato and tomato, revealing the biosynthesis and secretion of HFs. SGAs, such as α-solanine, α-chaconine, and α-tomatine, showed significant HS activity, despite being remarkably less activities than that of SEA. Then, we evaluated the contribution of SGAs on the HS activities of the hairy root culture media. The estimated SGAs content in the hairy root culture media were low and nonconcordant with the HS activity of those, suggesting that the HS activity of SGAs did not contribute much. The analysis of structureactivity relationship revealed that the structural requirements of the HS activity of SGAs are dependent on the sugar moieties attached at the C3-hydoroxyl group and the alkaloid property of their aglycones. The stereochemistry in the EF rings of their aglycone also affected the strength of the HS activity.

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Section: Hs Activity Of Potato and Tomato Hairy Root Culture Mediasupporting

confidence: 84%

Hatching stimulation activity of steroidal glycoalkaloids toward the potato cyst nematode, <i>Globodera rostochiensis</i>

Shimizu

Kushida

Akiyama

et al. 2020

Plant Biotechnology

View full text Add to dashboard Cite

show abstract

Production of Potato Cyst Nematode Hatching Stimulus by Hairy Root Cultures of Tomato

Cited by 1 publication

References 2 publications

Hatching stimulation activity of steroidal glycoalkaloids toward the potato cyst nematode, <i>Globodera rostochiensis</i>

Hatching stimulation activity of steroidal glycoalkaloids toward the potato cyst nematode, <i>Globodera rostochiensis</i>

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