Production of Proinflammatory Cytokines and Alpha-2-Macroglobulin by Peripheral Blood Cells in the Patients With Colorectal Cancer

Зорина, В. Н.; Промзелева, Н. В.; Зорин, Н. А.; Рябичева, Т. Г.; Зорина, Р. М.

doi:10.15789/1563-0625-2016-5-483-488

Cited by 5 publications

(7 citation statements)

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“…Other authors also observed an elevation in IL-6 and TNFα levels in the blood serum of colorectal cancer patients, with a subsequent decrease post-surgery by 40-60% [49].…”

Section: Group Amentioning

confidence: 80%

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Autoprobiotics in the treatment of patients with colorectal cancer in the early postoperative period

Ermolenko,

Baryshnikova,

Alekhina

et al. 2024

Preprint

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Introduction: Despite great advances in the treatment of oncological diseases, the development of medical technologies to prevent or reduce complications of therapy, in particular, those associated with surgery and the introduction of antibiotics remains relevant. The aim of the study: to evaluate the effectiveness of the use of autoprobiotics based on indigenous non-pathogenic strains of Enterococcus faecium and Enterococcus hirae in the complex therapy of colorectal cancer (CRC) in the early postoperative period. Materials and methods: A total of 36 patients diagnosed with CRC were enrolled in the study. The study group (Group A) comprised 24 CRC patients who received autoprobiotic therapy in the early postoperative period, while the control group (Group C) included 12 CRC patients without autoprobiotic therapy.Prior to surgery and between days 14-16 post-surgery, comprehensive evaluations were conducted on all patients, encompassing the following: stool and gastroenterological complains analysis. examination of the gut microbiota (bacteriological study, quantitative polymerase chain reaction, metagenome analysis), analysis of interleukins in serum. Results: The use of autoprobiotics led to decrease dyspeptic complaints after surgery, was associated with absence of postoperative complications, did not cause side effects, led to a decrease in the level of pro-inflammatory cytokines (IL-6 and IL-18) in the blood serum. The use of autoprobiotics led to positive changes in the structure of escherichia and enterococci populations, the elimination of Parvomonas micra, Fusobacterium nucleatum, decrease in the quantitative content of Clostridium perfringens, Akkermansia muciniphila. Metagenomic analysis (16S rRNA) revealed an increase in alpha diversity. Conclusion: The administration of autoprobiotics in the postoperative period is highly effective and safe in the comprehensive treatment of colorectal cancer patients. Future studies will allow the discovery of additional fine mechanisms of autoprobiotic therapy and its impact on the digestive, immune, endocrine and neural systems.

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“…Other authors also observed an elevation in IL-6 and TNFα levels in the blood serum of colorectal cancer patients, with a subsequent decrease post-surgery by 40-60% [49].…”

Section: Group Amentioning

confidence: 80%

“…Group C V1 V2 microorganisms beyond the gut mucosa [49][50][51]. Studies have reported negative prognoses for CRC with elevated IL-6 and TNFα levels in peritoneal fluid during the postoperative period [52].…”

Section: Group Amentioning

confidence: 99%

Autoprobiotics in the treatment of patients with colorectal cancer in the early postoperative period

Ermolenko,

Baryshnikova,

Alekhina

et al. 2024

Preprint

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“…Известно, что в патогенезе КРР значительную роль играют провоспалительные цитокины и гормоны. Негативным прогностическим фактором при КРР является избыток хорионического гонадотропина человека, провоцирующего гиперсекрецию TNF-α и IL-6, а также синтез IL-8 и матриксных металлопротеиназ, способствующих инвазии патогенных микроорганизмов за пределы слизистой оболочки кишечника [52][53][54]. Опубликованы данные по негативному прогнозу КРР при высоком содержании IL-6 и TNF-α в перитонеальной жидкости в послеоперационном периоде [55].…”

Section: результаты исследования 1 клинико-лабораторные данныеunclassified

“…Другими авторами также отмечено увеличение содержания IL-6 и TNF-α в сыворотке крови больных КРР и их снижение после операции на 40-60% [52]. Нельзя исключить, что наблюдаемые нами изменения после аутопробиотической поддержки связаны не только с базовой комплексной терапией КРР, но и с антивоспалительным действием аутопробиотиков, оказавшимися влияние на иммунитет и микробиоту [36].…”

Section: результаты исследования 1 клинико-лабораторные данныеunclassified

Gut microbiota in the combined treatment of colorectal cancer using autoprobiotics

Ermolenko,

Alyokhina,

Kashchenko

et al. 2024

jour

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Despite great advances in the treatment of oncological diseases, the development of medical technologies to prevent or reduce complications of therapy, in particular, those associated with surgery and the introduction of antibiotics, remains relevant. The aim of the study was to evaluate the effectiveness of the use of autoprobiotics based on indigenous non-pathogenic strains of Enterococcus faecium and E. hirae in the complex therapy of colorectal cancer (CRC) in the early postoperative period. The use of autoprobiotics did not cause side effects and led to a decrease in the level of pro-inflammatory cytokines (IL-6 and IL-18) in the blood serum. The features of the intestinal microbiome in patients with CRC were revealed, which are fundamentally different from those of other authors by an increase in alpha- biodiversity, lactobacilli, bifidobacteria, Bacteroides thetaiotaomicron and its additional differences associated with the absence of non-pathogenic enterococci: a greater amount of Parvimonas micra, a smaller amount of Akkermansia spp., an increase in the content of Klebsiella sp., Fusobacterium nucleatum and Clostridium perfringens. The use of autoprobiotics led to the elimination of P. micra, a decrease in C. perfringens, which led to the normalization of the microbiota in most patients. The effectiveness of autoprobiotics in the treatment of CRC has been proven, which, apparently, is associated with a change in the composition of intestinal microbiocenosis.

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“…В основе этих видов анализа лежит связывание с моноклональными антителами, фиксированными на поверхности микрочастиц или микрошариков. Проведенный нами анализ отечественной научной литературы иммунологического профиля на примере двух российских журналов «Медицинская иммунология» и «Инфекция и иммунитет» показал, что наибольшее распространение при проведении клинико-иммунологических исследований с определением содержания цитокинов в сыворотке/плазме крови получил твердофазный ИФА [4,6,7,13,14,15,16,17,23,25,26,27,28,29,31, 32] и мультиплексный иммуноанализ в формате хМАР- [2,9,19,20,21,22,30] и СВА-технологий [3,18].…”

Section: цитокины в периферической крови здоровыхunclassified

Methodological Issues of Determining Concentrations of Some Cytokines in Peripheral Mblood From Healthy Individuals

Arsentieva

Тотолян

2018

Med. immunol.

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Cytokines are the most important factors in pathogenesis of infectious, allergic, autoimmune, lymphoproliferative diseases and immunopathological processes. Many cytokines are very useful therapeutic targets for immunodiagnostics of different human diseases. Measurement of the cytokine levels by immunochemical methods in various biological fluids is usually used for diagnostic evaluation. Content analysis of research articles from two Russian immunological journals, “Meditsinskaya Immunologiya” = “Medical Immunology (Russia)” and “Infektsiya i immunitet” = “Russian Journal of Infection and Immunity,”, shows that ELISA, xMAP multiplex immunoassay, and CBA technologies are the most common methods used in clinical and immunological studies aimed for determination of cytokine contents in blood serum/plasma. Normal ranges of some plasma/serum cytokines in healthy individuals were subject to wide variations when using different methods and specific reagents from various manufacturers. The normal ranges applied by the CBA-technology, are significantly higher than appropriate values obtained by ELISA or xMAP-technologies. Most studies included a small control group, usually limited by 15-20 persons. In most of these works, blood serum samples were used for assays, whereas EDTA-conserved plasma was taken only in few studies. It has been concluded that the results of cytokine measurements in blood serum/plasma in healthy individuals vary in wide ranges, and depend on many factors, e.g., initial sampling material, mode of technology, type of test systems, and characteristics of the group under study: number of patients, age, gender, geographical factor, etc. The mentioned data demonstrate a need for large-scale multicenter clinical studies, in order to standardize measurements of the cytokine levels in human peripheral blood and to specify their normal values.

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Production of Proinflammatory Cytokines and Alpha-2-Macroglobulin by Peripheral Blood Cells in the Patients With Colorectal Cancer

Cited by 5 publications

References 5 publications

Autoprobiotics in the treatment of patients with colorectal cancer in the early postoperative period

Autoprobiotics in the treatment of patients with colorectal cancer in the early postoperative period

Gut microbiota in the combined treatment of colorectal cancer using autoprobiotics

Methodological Issues of Determining Concentrations of Some Cytokines in Peripheral Mblood From Healthy Individuals

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