Production of prostaglandin E2 in monocytes stimulated in vitro by Chlamydia trachomatis, Chlamydophila pneumoniae, and Mycoplasma fermentans

Krausse-Opatz, Birgit; Schmidt, Cornelia; Fendrich, Ursula; Bialowons, Anke; Kaever, Volkhard; Zeidler, Henning; Kuipers, Jens G.; Köhler, Lars

doi:10.1016/j.micpath.2004.06.007

Cited by 12 publications

(15 citation statements)

References 32 publications

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“…In our model, eicosanoids produced by this pathway were increasingly obvious with increasing challenge doses indicating that the AA cascade became more intensively involved in pulmonary host response as the chlamydial inoculum increased. This finding is in good agreement with in vitro data obtained in human monocytes showing that the amount of synthesized eicosanoids was dependent on the chlamydial multiplicity of infection [60]. While in vitro studies focused mainly on PGE2, in vivo data of our study revealed that concentrations of at least four eicosanoids (TXB2, 15-HETE, 12-HETE, and PGE2) increased with chlamydial load in lung tissue.…”

Section: Discussionsupporting

confidence: 92%

A Bovine Model of Respiratory Chlamydia psittaci Infection: Challenge Dose Titration

et al. 2012

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This study aimed to establish and evaluate a bovine respiratory model of experimentally induced acute C. psittaci infection. Calves are natural hosts and pathogenesis may resemble the situation in humans. Intrabronchial inoculation of C. psittaci strain DC15 was performed in calves aged 2–3 months via bronchoscope at four different challenge doses from 106 to 109 inclusion-forming units (ifu) per animal. Control groups received either UV-inactivated C. psittaci or cell culture medium. While 106 ifu/calf resulted in a mild respiratory infection only, the doses of 107 and 108 induced fever, tachypnea, dry cough, and tachycardia that became apparent 2–3 days post inoculation (dpi) and lasted for about one week. In calves exposed to 109 ifu C. psittaci, the respiratory disease was accompanied by severe systemic illness (apathy, tremor, markedly reduced appetite). At the time point of most pronounced clinical signs (3 dpi) the extent of lung lesions was below 10% of pulmonary tissue in calves inoculated with 106 and 107 ifu, about 15% in calves inoculated with 108 and more than 30% in calves inoculated with 109 ifu C. psittaci. Beside clinical signs and pathologic lesions, the bacterial load of lung tissue and markers of pulmonary inflammation (i.e., cell counts, concentration of proteins and eicosanoids in broncho-alveolar lavage fluid) were positively associated with ifu of viable C. psittaci. While any effect of endotoxin has been ruled out, all effects could be attributed to infection by the replicating bacteria. In conclusion, the calf represents a suitable model of respiratory chlamydial infection. Dose titration revealed that both clinically latent and clinically manifest infection can be reproduced experimentally by either 106 or 108 ifu/calf of C. psittaci DC15 while doses above 108 ifu C. psittaci cannot be recommended for further studies for ethical reasons. This defined model of different clinical expressions of chlamydial infection allows studying host-pathogen interactions.

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Section: Discussionsupporting

confidence: 92%

A Bovine Model of Respiratory Chlamydia psittaci Infection: Challenge Dose Titration

et al. 2012

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“…Mycoplasma presence due to cell culture contamination is able to efficiently and rapidly degrade extracellular amyloid-beta peptide. Mycoplasma fermentans also stimulated prostaglandin E2 production, and Mycoplasma hyorhinis infection exerted a significant impact on L-arginine metabolism [11–13]. Additionally, dendritic cells can sense mycoplasma infection and mature as they do in response to most viruses and bacteria, indicating that mycoplasma contamination could induce dendritic cell maturation [14, 15].…”

Section: Introductionmentioning

confidence: 99%

Metabolomics reveals mycoplasma contamination interferes with the metabolism of PANC-1 cells

Yu¹,

Wang²,

Zhang³

et al. 2016

Anal Bioanal Chem

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Mycoplasma contamination is a common problem in cell culture and can alter cellular functions. Since cell metabolism is either directly or indirectly involved in every aspect of cell function, it is important to detect changes to the cellular metabolome after mycoplasma infection. In this study, liquid chromatography mass spectrometry (LC/MS)-based metabolomics was used to investigate the effect of mycoplasma contamination on the cellular metabolism of human pancreatic carcinoma cells (PANC-1). Multivariate analysis demonstrated that mycoplasma contamination induced significant metabolic changes in PANC-1 cells. Twenty-three metabolites were identified and found to be involved in arginine and purine metabolism and energy supply. This study demonstrates that mycoplasma contamination significantly alters cellular metabolite levels, confirming the compelling need for routine checking of cell cultures for mycoplasma contamination, particularly when used for metabolomics studies. Graphical abstract Metabolomics reveals mycoplasma contamination changes the metabolome of PANC-1 cells.

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“…This is known to stimulate COX‐2 expression and is also associated with enhanced histone acetylation linked with promoter regions of pro‐inflammatory mediators [13]. Up‐regulation of COX‐2 expression in response to histone hyperacetylation has been reported in vitro [14,15].…”

Section: Introductionmentioning

confidence: 99%

Elevated plasma prostaglandins and acetylated histone in monocytes in Type 1 diabetes patients

2009

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Results support increased inflammatory activity in Type 1 diabetes that involves COX-2 and increased prostaglandin production, which may predispose patients to cardiovascular events. The observation of elevated histone acetylation only in complication-free diabetic subjects suggests that this may be a protective mechanism. This merits further investigation as histone hyperacetylation has been associated with reduced expression of factors involved in vascular injury and remodelling.

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Production of prostaglandin E2 in monocytes stimulated in vitro by Chlamydia trachomatis, Chlamydophila pneumoniae, and Mycoplasma fermentans

Cited by 12 publications

References 32 publications

A Bovine Model of Respiratory Chlamydia psittaci Infection: Challenge Dose Titration

A Bovine Model of Respiratory Chlamydia psittaci Infection: Challenge Dose Titration

Metabolomics reveals mycoplasma contamination interferes with the metabolism of PANC-1 cells

Elevated plasma prostaglandins and acetylated histone in monocytes in Type 1 diabetes patients

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