Production of (R)-(−)-mandelic acid with nitrilase immobilized on D155 resin modified by l -lysine

Jiang, Wen; Tao, Renyou; Yang, Yang; Xu, Yan; Kang, Hongkuan; Zhou, Xiaohua; Zhou, Zhiming

doi:10.1016/j.bej.2017.07.010

Cited by 15 publications

(4 citation statements)

References 47 publications

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“…Then the solution was purified according to the purified method mentioned in the literature with some modification. 38 After that, the purifiedand -glucosaccharase were obtained.…”

Section: Preparation Of Viscose Fibers Modified With Spcosmentioning

confidence: 99%

Preparation of viscose fiber modified with silkworm pupa chitosan oligosaccharide by β-glucosaccharase

Jiang

Zhou

Su³

2019

Textile Research Journal

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Viscose fibers (VFs) modified with silkworm pupa chitosan oligosaccharide (SPCOS) by β-glucosaccharase were prepared in this study. Silkworm pupa underwent the processes of deproteinization, demineralization, decoloration and depolymerization. SPCOS with a DD of 91.64 ± 0.86% and Mw of (2.56 ± 0.15) × 104 g/mol was prepared. The prepared SPCOS was used for the modification of VFs. β-glucosaccharase was used as the enzyme for this modification. The results showed that VFs were successfully modified with SPCOS by β-glucosaccharase. The synthesized VFs modified with SPCOS (VF-SPCOS) were dyed more steadily by anionic dyes than cationic dyes. Furthermore, VF-SPCOS showed a good antibacterial property against E. coli and S. aureus, especially to E. coli. In the reuse study of SPCOS, the antibacterial property to E. coli of VF-SPCOS was still retained at 87.68 ± 1.18% of its initial value after the 12th reuse of SPCOS.

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“…Then the solution was purified according to the purified method mentioned in the literature with some modification. 38 After that, the purifiedand -glucosaccharase were obtained.…”

Section: Preparation Of Viscose Fibers Modified With Spcosmentioning

confidence: 99%

Preparation of viscose fiber modified with silkworm pupa chitosan oligosaccharide by β-glucosaccharase

Jiang

Zhou

Su³

2019

Textile Research Journal

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“…Then the NAL solution was purified using the purification method mentioned in the literature with some modification. 35 Depolymerization of Radish Procyanidin Polymers by NAL. The radish procyanidin polymers (RPP) powder (5 g) was poured into 100 mL of distilled water to prepare 50 mg/mL RPP solution.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…In total, 10 g of N -acetylneuraminate lyase (NAL) was first poured into 1000 mL of aqueous solution. Then the NAL solution was purified using the purification method mentioned in the literature with some modification …”

Section: Methodsmentioning

confidence: 99%

Integrated Utilization of Red Radish for the Efficient Production of High-Purity Procyanidin Dimers

Jiang

Zhou

Yang

et al. 2018

J. Agric. Food Chem.

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Red radish was extracted by methanol to obtain crude radish procyanidin extracts. The purity of procyanidin (PP) and procyanidin dimers (PD) of crude radish procyanidin extracts under different ratios of methanol to radish was optimized to achieve the best extraction performance. Then the crude radish procyanidin extracts was, respectively, processed six macroporous resins separation to separate radish procyanidin oligomers (RPO) and polymers (RPP). Depolymerization of radish procyanidin polymers (RPP) into oligomers was then conducted. N-Acetylneuraminate lyase (NAL) was first used as the enzyme to depolymerize RPP. The depolymerization yield (DY) under different depolymerized conditions was also investigated. Results showed the DY of RPP would achieve 53.24 ± 0.35% at the best conditions. Then the high-purity procyanidin dimers was prepared by depolymerized procyanidin oligomers and PRO. Additionally, the chemical structure of the preparative radish procyanidin dimers was elucidated by high-resolution mass spectrometry and one- and two-dimensional NMR.

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“…Recently, several methods for biosynthesis of ( R )-MA are reported. The single step biosynthesis of ( R )-MA is mainly based on the kinetic resolution of racemic mandelonitrile with nitrilases (NLases) and the kinetic resolution of racemic MA using enzymes such as, lipases, esterases, or dehydrogenases (Chen et al 2017 ; Detzel et al 2011 ; He et al 2007 ; Jiang et al 2017 , 2016 ; Li et al 2007 ; Wang et al 2015 ; Xiao et al 2005 ). These methods usually suffer from low efficiency and low yield, and the racemic feedstocks are still derived from chemical synthesis.…”

Section: Introductionmentioning

confidence: 99%

Production of (R)-mandelic acid from styrene, L-phenylalanine, glycerol, or glucose via cascade biotransformations

Lukito

Wang

Sekar

et al. 2021

Bioresour. Bioprocess.

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Abstract(R)-mandelic acid is an industrially important chemical, especially used for producing antibiotics. Its chemical synthesis often uses highly toxic cyanide to produce its racemic form, followed by kinetic resolution with 50% maximum yield. Here we report a green and sustainable biocatalytic method for producing (R)-mandelic acid from easily available styrene, biobased L-phenylalanine, and renewable feedstocks such as glycerol and glucose, respectively. An epoxidation-hydrolysis-double oxidation artificial enzyme cascade was developed to produce (R)-mandelic acid at 1.52 g/L from styrene with > 99% ee. Incorporation of deamination and decarboxylation into the above cascade enables direct conversion of L-phenylalanine to (R)-mandelic acid at 913 mg/L and > 99% ee. Expressing the five-enzyme cascade in an L-phenylalanine-overproducing E. coli NST74 strain led to the direct synthesis of (R)-mandelic acid from glycerol or glucose, affording 228 or 152 mg/L product via fermentation. Moreover, coupling of E. coli cells expressing L-phenylalanine biosynthesis pathway with E. coli cells expressing the artificial enzyme cascade enabled the production of 760 or 455 mg/L (R)-mandelic acid from glycerol or glucose. These simple, safe, and green methods show great potential in producing (R)-mandelic acid from renewable feedstocks.

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Production of (R)-(−)-mandelic acid with nitrilase immobilized on D155 resin modified by l -lysine

Cited by 15 publications

References 47 publications

Preparation of viscose fiber modified with silkworm pupa chitosan oligosaccharide by β-glucosaccharase

Preparation of viscose fiber modified with silkworm pupa chitosan oligosaccharide by β-glucosaccharase

Integrated Utilization of Red Radish for the Efficient Production of High-Purity Procyanidin Dimers

Production of (R)-mandelic acid from styrene, L-phenylalanine, glycerol, or glucose via cascade biotransformations

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