Production of raw cassava starch-degrading enzyme by Penicillium and its use in conversion of raw cassava flour to ethanol

Lin, Hai-Juan; Liu, Xian; Zhang, Qiu-Jiang; Luo, Xi; Xu, Qiangsheng; Yang, Qi; Duan, Chengjie; Liu, Junliang; Tang, Ji‐Liang; Feng, Jia‐Xun

doi:10.1007/s10295-010-0910-7

Cited by 45 publications

(31 citation statements)

References 27 publications

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“…On the other hand, on-site production of multiactivity enzyme preparations from Penicillium sp. [11] and A. niger [20] for use in the hydrolysis of raw cassava starch has been reported as a potential improvement to the SSF process. However, because on-site manufacturing of multi-activity enzyme preparations is a relatively new concept, they have only been evaluated on a laboratory scale (e.g., 250-ml Erlenmeyer Xasks).…”

Section: Introductionmentioning

confidence: 99%

Production of multiple extracellular enzyme activities by novel submerged culture of Aspergillus kawachii for ethanol production from raw cassava flour

Sugimoto

Makita

Watanabe

et al. 2012

Journal of Industrial Microbiology and Biotechnology

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Cassava is a starch-containing root crop that is widely used as a raw material in a variety of industrial applications, most recently in the production of fuel ethanol. In the present study, ethanol production from raw (uncooked) cassava flour by simultaneous saccharification and fermentation (SSF) using a preparation consisting of multiple enzyme activities from Aspergillus kawachii FS005 was investigated. The multi-activity preparation was obtained from a novel submerged fermentation broth of A. kawachii FS005 grown on unmilled crude barley as a carbon source. The preparation was found to consist of glucoamylase, acid-stable α-amylase, acid carboxypeptidase, acid protease, cellulase and xylanase activities, and exhibited glucose and free amino nitrogen (FAN) production rates of 37.7 and 118.7 mg/l/h, respectively, during A. kawachii FS005-mediated saccharification of uncooked raw cassava flour. Ethanol production from 18.2% (w/v) dry uncooked solids of raw cassava flour by SSF with the multi-activity enzyme preparation yielded 9.0% (v/v) of ethanol and 92.3% fermentation efficiency. A feasibility study for ethanol production by SSF with a two-step mash using raw cassava flour and the multi-activity enzyme preparation manufactured on-site was verified on a pilot plant scale. The enzyme preparation obtained from the A. kawachii FS005 culture broth exhibited glucose and FAN production rates of 41.1 and 135.5 mg/l/h, respectively. SSF performed in a mash volume of about 1,612 l containing 20.6% (w/v) dry raw cassava solids and 106 l of on-site manufactured A. kawachii FS005 culture broth yielded 10.3% (v/v) ethanol and a fermentation efficiency of 92.7%.

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Section: Introductionmentioning

confidence: 99%

Production of multiple extracellular enzyme activities by novel submerged culture of Aspergillus kawachii for ethanol production from raw cassava flour

Sugimoto

Makita

Watanabe

et al. 2012

Journal of Industrial Microbiology and Biotechnology

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“…Vários autores já conseguiram expressar genes de glicoamilases de fungos filamentosos em S. cerevisiae, tais como Aspergillus awamorii GA1 (LIN et al, 1998), Aspergillus awamori Sga1 (FAVARO et al, 2010), Aspergillus oryzae GlaA (KOTAKA et al, 2008), Rhyzopus oryzae GAII (ASHIKARI et al, 1989), Rhizopus arrhizus GA (YANG et al, 2011). No entanto, nenhum desses autores relata o potencial destas glicoamilases degradarem amido bruto de mandioca.…”

Section: Resultsunclassified

“…No presente trabalho, a S. cerevisiae empregada é uma cepa haploide, mas que produziu 4,22 g/L em 96 horas de fermentação usando 20 g/L de um substrato altamente impuero e não solúvel que é o amido industrial de mandioca o que tem um apelo maior por ser de baixo custo. Lin et al (2011) descreveram o potencial do fungo Penicillium sp. GXU20 em produzir enzimas de degradação de amido bruto de mandioca para a produção de etanol durante 6 dias de cultivo.…”

Section: Resultsunclassified

Hidrólise enzimática do amido de mandioca e produção de bioetanol por Saccharomyces cerevisiae CENPK2 expressando uma glicoamilase de Aspergillus awamori

Marques¹,

Coutinho²,

Ribeiro³

et al. 2013

BBR - Biochem. Biotechnol.

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This study aimed to verify the hydrolysis of starch raw cassava using a glucoamylase from Aspergillus awamori expressed in Saccharomyces cerevisiae. The glucoamylase activity test plate showed that S. cerevisiae recombinant is able to degrade cassava starch without the needs for either acid pretreatment or cooking. The recombinant yeast was able to form 2.7 g / L of biomass and degrades cassava starch up to 90%, releasing a large amount of glucose to the culture medium. The N-deglycosylation assay showed that the recombinant glucoamylase (75 kDa) has 10% carbohydrate in their structure compared with enzyme treated (65 kDa). Our preliminary results showed that recombinant yeast was able to produce 4.22 g/L in 96 hours fermentation. The results of starch hydrolysis shown that S. cerevisiae recombinant has a great potential for producing ethanol from cassava starch without the need for pretreatment of the substrate. RESUMOEsse trabalho teve como objetivo verificar a hidrólise do amido bruto de mandioca, usando a glicoamilase de Aspergillus awamori expressa em Saccharomyces cerevisiae. O teste de atividade de glicoamilase em placa mostrou que a S. cerevisiae recombinante é capaz de degradar o amido de mandioca sem a necessidade de pré-tratamento acido ou cozimento. A levedura recombinante foi capaz de formar 2,7 g/L de biomassa que degradou 90% do amido, liberando uma grande quantidade de glicose para o meio de cultura. O ensaio de deglicosilação mostrou que a glicoamilase recombinante (75 kDa) possui 10% de carboidrato na sua estrutura comparado com a enzima deglicosilada (65 kDa). Nossos resultados preliminares mostraram que a levedura recombinante produziu 4,22 g/L de bioetanol em 96 horas de fermentação. Os resultados da hidrólise do amido mostraram que a S. cerevisiae recombinante utilizada nesse trabalho tem um grande potencial de produzir etanol a partir de amido de mandioca sem a necessidade de um pré-tratamento do substrato.

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“…Cassava (Manihot esculenta Cranz), the world's fourth most important crop, is grown in many countries in Africa, Asia, and Latin America, and is widely employed as a raw material for many industrial applications, such as animal feed, starch, and biofuel [24]. Additionally, cassava has a strong adaptability and can grow on arid and semiarid land, and contains nearly the maximum theoretical concentration of starch on a dry weight basis for a food crop [7].…”

Section: Introductionmentioning

confidence: 99%

High-Level Butanol Production from Cassava Starch by a Newly Isolated Clostridium acetobutylicum

Guo

et al. 2015

Appl Biochem Biotechnol

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A new Clostridium acetobutylicum strain, exhibiting the ability to resist butanol stress and produce butanol, was identified and named GX01. Strain GX01 can use a wide variety of carbohydrates, especially cassava starch, to produce butanol. After the optimization of culture conditions, C. acetobutylicum GX01 could produce 27.3 g/L solvent, including 17.1 g/L butanol, 7.9 g/L acetone, and 2.3 g/L ethanol, from 100 g/L cassava flour and 3 g/L soybean meal. Furthermore, when its acetone-butanol-ethanol (ABE) fermentation was performed in 10- and 30-L bioreactors, the production of total solvent and butanol reached 29.2 and 18.3 g/L, respectively, and 28.8 and 18.8 g/L, respectively. Thus, the high level and stability of butanol production make strain GX01 a promising candidate for ABE fermentation using the low-cost cassava starch.

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Production of raw cassava starch-degrading enzyme by Penicillium and its use in conversion of raw cassava flour to ethanol

Cited by 45 publications

References 27 publications

Production of multiple extracellular enzyme activities by novel submerged culture of Aspergillus kawachii for ethanol production from raw cassava flour

Production of multiple extracellular enzyme activities by novel submerged culture of Aspergillus kawachii for ethanol production from raw cassava flour

Hidrólise enzimática do amido de mandioca e produção de bioetanol por Saccharomyces cerevisiae CENPK2 expressando uma glicoamilase de Aspergillus awamori

High-Level Butanol Production from Cassava Starch by a Newly Isolated Clostridium acetobutylicum

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