Production of reactive oxygen species and development of antioxidative systems during in vitro growth and ex vitro transfer

Baťková, P.; Pospíšilová, Jana; Synková, Helena

doi:10.1007/s10535-008-0085-5

Cited by 84 publications

(36 citation statements)

References 115 publications

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“…Each parameters were measured before and after ex vitro transfer. Means ± SE, n = 20 cuticle deposition on cells, sudden dehydration and increase in ROS production (Batkova et al 2008). In vitro cultured plantlets are characterized by deficient functionality of stomata, which is gradually recovered during ex vitro acclimatization (Dias et al 2013a, b;Hazarika 2006;Pospisilova et al 2009b).…”

Section: Resultsmentioning

confidence: 99%

Improvement ofex vitroacclimatization of mulberry plantlets by supplement of abscisic acid to the last subculture medium

2017

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Mulberry (Morus sp.) of the family Moraceae is very economically important in Asian countries including Korea, because its leaf and fruit have been commercially used in sericulture and horticultural industries. Therefore it is necessary to develop the optimal production system for rapid and cost-effective propagation of mulberry. Our studies focused on establishing an acclimatization method for the successful plantlet production of new cultivar 'Cheongsu' which was transferred ex vitro after in vitro culture. In particular, effect of abscisic acid (ABA) addition into the last subculture medium on plantlet response to subsequent ex vitro transfer and its growth was investigated. During acclimatization, stomatal conductance and transpiration rate of ABA-pretreated plantlets were significantly lower than those of non-treated plantlets. Net photosynthetic rate of ABA-pretreated plantlets decreased after ex vitro transfer but increased after 14 days, and it was mostly higher than that of non-treated plantlets. Moreover, relative water content as well as chlorophyll contents and its ratio were also higher in ABA-pretreated plantlets. On the other hand, proline was considerably higher than in control plantlets. After 1 month of ex vitro transfer, survival rate of ABA-pretreated plantlets was 85.6%, which increased by 29.1% in comparison with control (56.5%). More vigorous growth was also observed in ABA-pretreated plantlets. From these results, it was found that application of ABA to the last subculture medium could improve acclimatization and promote survival of mulberry plantlets after ex vitro transfer, inducing water stress tolerance and alleviating abiotic stresses.Keywords Mulberry, Abscisic acid, Ex vitro transfer, Acclimatization, Water stress Introduction Mulberry (Morus sp.) is an economically important tree grown in India, China, Korea and several Asian countries where its foliage is used as food for silkworms in sericulture industry (Vijayan et al. 2012). It is also commercially valuable in the horticulture, food and cosmetic industries, in particular, its phytochemical and medicinal properties such as antioxidants (Yen et al. 1996) and hypoglycemia compounds (Kelkar et al. 1996) have been widely used as healthcare products. These days it is cultivated for fruit production, which is used for human consumption including jam, jelly, frozen desserts, pulp, juice and wine (Koyuncu 2004). Mulberry fruit is regarded as a traditional medicine for dysentery, constipation and avulsed teeth due to rich phenolic acids and flavonoids (Arfan et al. 2012; Lee and Bae 2011).Plant micropropagation has been widely applied to agriculture industry for large scale production of economically important and valuable species. But its commercial use is limited in many species due to the low plantlet survival rates during the acclimatization (Pospisilova et al. 2009a, b), which are known to be related to an abnormal morphology, anatomy and physiology under in vitro culture conditions (Dias et al. 2013a). Poor functioning of...

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Section: Resultsmentioning

confidence: 99%

Improvement ofex vitroacclimatization of mulberry plantlets by supplement of abscisic acid to the last subculture medium

2017

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“…During acclimatization, the plantlets are subjected to several stresses in response to changes in environmental conditions, which occurs due to impaired stomata function and reduced cuticle deposition on cells, resulting in dehydration and increase in ROS production (Baťková et al, 2008). Means identified by the same letters (comparing the effects of sucrose concentration, in each growth regulator, for each type of closure) do not differ significantly by the Scott-Knott test at 5% probability.…”

Section: Discussionmentioning

confidence: 99%

“…Morphophysiological changes caused by in vitro culture, by light and due to hydric and osmotic stresses are the most important factors limiting plant acclimatization under field conditions, as they stimulate the oxidative metabolism by increasing the production of reactive oxygen species (ROS), especially the superoxide radicals (O 2˙-), hydrogen peroxide (H 2 O 2 ) and hydroxyl radicals (OH˙) (Baťková et al, 2008;Varshney and Anis, 2012). The O 2˙-spontaneously turns into H 2 O 2 , but this process is more efficient when catalyzed by superoxide dismutase (SOD).…”

Section: Introductionmentioning

confidence: 99%

Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)

et al. 2016

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This study aimed to evaluate the variation in the levels of proline, oxidative metabolism and photosynthetic pigments in plants of Pitcairnia encholirioides grown in vitro under different conditions and after acclimatization. The analyses were performed after 150 days of in vitro cultivation in MS media supplemented with 10 µM GA 3 or 0.2 µM NAA, sucrose at 15 or 30 g L -1 , in test tubes which allowed gas exchange or in a hermetically sealed system, and 180 days after acclimatization. The in vitro maintenance in hermetically sealed flasks, with GA 3 and 15 g L -1 sucrose had adverse metabolic effects, which was demonstrated by the lower proline and photosynthetic pigments accumulation and by the increase in antioxidant enzymes activities. After acclimatization, differences for proline and photosynthetic pigments were no longer found and the enzymatic activities ranged unevenly. The results suggest that the in vitro cultivation in media with 0.2 µM NAA and 30 g L -1 sucrose, in test tubes capped with closures which allowed gas exchange, is more suitable for micropropagation of P. encholirioides, providing a prolonged maintenance of in vitro cultures and plantlets with superior quality for ex vitro development.Keywords: enzymatic activity, oxidative stress, micropropagation, proline. Niveis de prolina, do metabolismo oxidativo e dos pigmentos fotossintéticos durante o crescimento in vitro e aclimatização de Pitcairnia encholirioides L.B. Sm. (Bromeliaceae) ResumoEste trabalho objetivou avaliar a contribuição da prolina, do metabolismo oxidativo e dos pigmentos fotossintéticos na propagação in vitro e aclimatização de Pitcairnia encholirioides, uma bromélia criticamente ameaçada de extinção. As análises foram realizadas após 150 dias de cultivo in vitro em meio MS suplementado com 10 µM de GA 3 ou 0,2 µM de ANA, 15 ou 30 g L -1 de sacarose, em tubos de ensaio que permitiam trocas gasosas ou em sistema hermeticamente vedado, e também 180 dias após aclimatização. A manutenção in vitro em frascos hermeticamente fechados, com GA 3 e 15 g L -1 de sacarose apresentou efeito metabólico adverso, demonstrado pelo menor acúmulo de prolina e pigmentos fotossintéticos e também pelo aumento das atividades de enzimas antioxidantes. Após aclimatização, as diferenças para prolina e pigmentos fotossintéticos não foram mais encontradas e as atividades enzimáticas variaram de maneira desuniforme. Os resultados sugerem que o cultivo in vitro em meio com 0,2 µM de ANA e 30 g L -1 de sacarose, em tubos fechados com tampas que permitem trocas gasosas, é mais adequado para a micropropagação de P. encholirioides, proporcionando uma manutenção prolongada das culturas in vitro e plântulas com qualidade superior para o desenvolvimento ex vitro.Palavras-chave: atividade enzimática, estresse oxidativo, micropropagação, prolina.

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“…Therefore, relevant studies give us physiological information on the levels of stress under standard in vitro conditions or at treatments with growth retardants [36]. (ii) The activity of antioxidant enzyme systems is relevant in pharmacological studies [37]. For several tissue cultures of this study, there were discrepancies between free radical scavenging capacity and peroxidase activities.…”

Section: Discussionmentioning

confidence: 91%

Production and antioxidant capacity of tissue cultures from four Amaryllidaceae species

et al. 2017

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The aim of this study was (i) to produce tissue cultures capable of efficient plant regeneration from European naturally occurring protected and/or pharmacologically important Amaryllidaceae species and (ii) to test them for antioxidant activities in order to select tissue cultures that scavenge efficiently oxygen radicals. Bulb explants were collected from Galanthus woronowii, two Leucojum species, and Sternbergia lutea. Leucojum species were Hungarian isolates. Mostly α-naphthalene acetic acid (NAA) and benzyladenine (BA) were used as growth regulator combinations for the induction and maintenance of tissue cultures and further antioxidant activity studies. Galanthus woronowii and L. vernum cultures produced shoots or whole plants via micropropagation (callus stage was observed only sporadically and callus tissue did not contribute to regeneration), whereas L. aestivum and S. lutea produced efficiently whole plants or multiple shoots via embryogenic calli. Total phenolic content, % inhibition of ABTS radical (ABTS*) cation, and peroxidase activities on native polyacrylamide gels were studied and showed differences between cultures. No relationship could be detected between polyphenol content / radical scavenging capacities and H 2 O 2 reducing enzyme activities. For G. woronowii, S. lutea, and a culture line of L. vernum, polyphenol content and ABTS* cation scavenging activities were high and for G. woronowii, comparable to organs of the native plants used as explant sources. Bulbs of native plants showed low radical scavenging activities in general. For L. vernum and L. aestivum tissue cultures grown in the presence of NAA as the sole growth regulator, ABTS* cation scavenging showed low values. Enzymatic antioxidant (pyrogallol peroxidase) activities were low for all cultures and organs of native plants. This study shows the species conservation value of these cultures and highlights the high antioxidant capacity of G. woronowii and S. lutea, attributed to the presence of non-enzymatic scavengers.

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Production of reactive oxygen species and development of antioxidative systems during in vitro growth and ex vitro transfer

Cited by 84 publications

References 115 publications

Improvement ofex vitroacclimatization of mulberry plantlets by supplement of abscisic acid to the last subculture medium

Improvement ofex vitroacclimatization of mulberry plantlets by supplement of abscisic acid to the last subculture medium

Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)

Production and antioxidant capacity of tissue cultures from four Amaryllidaceae species

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