Production of Recombinant Factor Viii From Perfusion Cultures: I. Large-Scale Fermentation

Bödeker, Berthold G. D.; Newcomb, Richard D.; Yuan, Panhong; Braufman, A.; Kelsey, Weston M.

doi:10.1016/b978-0-7506-1845-8.50133-2

Cited by 32 publications

(23 citation statements)

References 6 publications

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“…Increasing cell density can improve volumetric productivity if per cell productivity can be maintained at a maximal level. High cell density perfusion bioreactors have been one approach (Bodeker et al 1994;Caron et al, 1994;Silberklang et al, 1995;Tolbert et al, 1995). Unfortunately, the rate of perfusion necessary to maintain productivity at these high cell densities is usually significant and hence, expensive.…”

Section: Introductionmentioning

confidence: 97%

Effect of elevated oxygen and glutamine levels on foreign protein production at high cell densities using the insect cell-baculovirus expression system

Taticek

Shuler

1997

Biotechnol. Bioeng.

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Section: Introductionmentioning

confidence: 97%

Effect of elevated oxygen and glutamine levels on foreign protein production at high cell densities using the insect cell-baculovirus expression system

Taticek

Shuler

1997

Biotechnol. Bioeng.

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“…Extensive efforts have been made to maximize protein production in transient expression systems by optimization of host cell lines [9,11,[23][24][25][26], vector systems [27][28][29], and cell culture conditions [30,31]. Besides these factors, the cell culture medium and gene delivery reagents also play a critical role for efficient production of r-protein.…”

Section: Introductionmentioning

confidence: 99%

Transient Transfection Factors for High-Level Recombinant Protein Production in Suspension Cultured Mammalian Cells

Liu¹,

Dalby²,

Chen³

et al. 2008

Mol Biotechnol

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The efficient transfection of cloned genes into mammalian cells system plays a critical role in the production of large quantities of recombinant proteins (r-proteins). In order to establish a simple and scaleable transient protein production system, we have used a cationic lipid-based transfection reagent-FreeStyle MAX to study transient transfection in serum-free suspension human embryonic kidney (HEK) 293 and Chinese hamster ovary (CHO) cells. We used quantification of green fluorescent protein (GFP) to monitor transfection efficiency and expression of a cloned human IgG antibody to monitor r-protein production. Parameters including transfection reagent concentration, DNA concentration, the time of complex formation, and the cell density at the time of transfection were analyzed and optimized. About 70% GFP-positive cells and 50-80 mg/l of secreted IgG antibody were obtained in both HEK-293 and CHO cells under optimal conditions. Scale-up of the transfection system to 1 l resulted in similar transfection efficiency and protein production. In addition, we evaluated production of therapeutic proteins such as human erythropoietin and human blood coagulation factor IX in both HEK-293 and CHO cells. Our results showed that the higher quantity of protein production was obtained by using optimal transient transfection conditions in serum-free adapted suspension mammalian cells.

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“…Vero cells have been used for the production of viral vaccines for humans for more than ten years (Montagnon et al, 1984), whereas BHK-21 C13 cells are used for the production of veterinary vaccines (e.g. Capstick et al, 1962, Radlett 1987) and rather recently also for the production of recombined proteins for human therapy (BOdeker et al, 1994). Although both cell lines grow well in the SFM, they are not very interesting for the production of influenza virus, because the maximal titres obtainable in perfusion cultures ranged only between 256 and 512 HAU.…”

Section: Discussionmentioning

confidence: 99%

Production of Influenza Virus in Cell Cultures for Vaccine Preparation

Merten

Hannoun

Manuguerra

et al. 1996

Advances in Experimental Medicine and Biology

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Influenza virus strains of different types for use as an inactivated vaccine have been successfully grown in different cell lines. Increasing titres were obtained with BHK-2l IBRS, VERO and MDCK cells. Cultures in stationary flasks, in spinner cultures or in large bioreactor systems were tested and the optimal conditions were studied. MDCK cells grown in serum-free medium before and during the virus production phase were found to yield high titres in the presence of trypsin. Satisfactory results were obtained with egg-adapted strains of human and equine origin as well as with strains just isolated from human patients without any further passages in eggs or cell culture.

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Production of Recombinant Factor Viii From Perfusion Cultures: I. Large-Scale Fermentation

Cited by 32 publications

References 6 publications

Effect of elevated oxygen and glutamine levels on foreign protein production at high cell densities using the insect cell-baculovirus expression system

Effect of elevated oxygen and glutamine levels on foreign protein production at high cell densities using the insect cell-baculovirus expression system

Transient Transfection Factors for High-Level Recombinant Protein Production in Suspension Cultured Mammalian Cells

Production of Influenza Virus in Cell Cultures for Vaccine Preparation

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