Production of recombinant human relaxin 3 in AtT20 cells

Relaxin-3/INSL7 Regulates the Stress-response System in the Rat Hypothalamus

“…Rats which showed an orexigenic response after icv injection of Neuropeptide Y (5 μg) 1 day before were used for the experiments. In the middle of the light phase (1400-1600 hours), 10 μl of saline with or without 1 nmol human RLN3 (provided by Dr. Nishi, Takeda Pharmaceutical Company Ltd, Kizawa et al 2003) was injected into the animals at a flow rate of 2.5 μl/min through a microinjection cannula (AMI-13, EICOM) that was inserted into the guide cannula.…”

International Union of Pharmacology LVII: Recommendations for the Nomenclature of Receptors for Relaxin Family Peptides

“…Recently, another human insulin/relaxin-like molecule and its nearly identical orthologs in the mouse, rat, 1 Abbreviations: RLN1, gene encoding H1 relaxin; RLN2, gene encoding H2 relaxin; H1 relaxin, human gene 1 relaxin; H2 relaxin, human gene 2 relaxin; H3 relaxin, human gene 3 relaxin; (species) relaxin-3, peptide corresponding to human gene 3 (H3) relaxin in other species; Ley-I-L, Leydig insulin-like peptide; INSL3, gene encoding insulin-like peptide 3; INSL4, gene encoding insulin-like peptide 4; INSL5, gene encoding insulin-like peptide 5; INSL6, gene encoding insulin-like peptide 6; RLF, relaxin-like factor; INSL3, insulin-like peptide 3; INSL4, insulin-like peptide 4; INSL5, insulinlike peptide 5; INSL6, insulin-like peptide 6; LGR, leucine-rich repeat-containing guanine nucleotide binding (G-protein)-coupled receptors; FSH, follicle-stimulating hormone; LH, luteinizing hormone; TSH, thryroid-stimulating hormone; Great, G-protein coupled receptor affecting testis descent; LGR7, leucine-rich repeatcontaining guanine nucleotide binding (G-protein)-coupled receptor 7; LGR8, leucine-rich repeat-containing guanine nucleotide binding (G-protein)-coupled receptor 8; GPCR, G-protein coupled receptor; GPCR135, G-protein coupled receptor 135 (orphan receptor nomenclature); GPCR142, G-protein coupled receptor 142 (orphan receptor nomenclature); RXFP1, relaxin family peptide receptor 1; RXFP2, relaxin family peptide receptor 2; RXFP3, relaxin family peptide receptor 3; RXFP4, relaxin family peptide receptor 4; RT-PCR, reverse transcription-polymerase chain reaction; PKA, protein kinase A; SFO, subfornical organ; OVLT, organum vasculosum of the lamina terminalis; ET 1-32 , first 32 amino acids of endothelin peptide; NO, nitric oxide; NOS, nitric-oxide synthase; THP-1, human monocytic cell line; LRR, leucine-rich repeat; LDLa, low-density lipoprotein class A; TM, transmembrane; PKC, protein kinase C; MAPK, mitogen-activated protein kinase; PDE, phosphodiesterase; HEK293, human embryonic kidney (cells); LY294002, 2-(4-morpholinyl)-8-phenyl-1(4H)-benzopyran-4-one hydrochloride; PI3-K, phosphatidyinositol 3 kinase; Erk1/2, extracellular signalling regulated kinase; GR, glucocorticoid receptor; RU486, 17␤-hydroxy-11␤-[4-dimethylamino phenyl]-17␣-[1-propynyl]estra-4,9-dien-3-one. 8 and pig were discovered (Bathgate et al, 2002a;Burazin et al, 2002;Kizawa et al, 2003). Because this peptide has the amino acid motif Arg-X-X-X-Arg-X-X-Ile required for relaxin bioactivity in the middle of the B chain and it activates the relaxin receptor (Sudo et al, 2003), it was designated relaxin-3 or in the case of the human relaxin-3 peptide, H3 relaxin ( Fig.…”

Relaxin Regulates MMP Expression and Promotes Satellite Cell Mobilization During Muscle Healing in Both Young and Aged Mice

“…C2C12 myoblasts (purchased from ATCC, Manassas, VA) or primary human myoblasts (obtained from our stem cell center) were treated with or without relaxin (1000 ng/ml of porcine relaxin from Sky BioHealth Solutions Inc, St. Paul, MN; this relaxin had been verified for its effect on cAMP production in THP1 cells 47 ) and cultured in differentiation medium [DMEM (Invitrogen, Carlsbad, CA) supplemented with 2% Horse Serum (HS), 1% PenicillinStreptomycin antibiotics (P/S)], and incubated in 5% CO 2 at 37°C. MMP blocker GM6001 (Millipore, Billerica, MA) was applied at 10 mol/L.…”