Production of recombinant Potato mop-top virus coat protein in Escherichia coli and generation of antisera recognising native virus protein

Hélias, Valerie; Jacquot, Emmanuel; Guillet, Maryse; Hingrat, Yves; Giblot‐Ducray, D.

doi:10.1016/s0166-0934(03)00105-8

Cited by 8 publications

(6 citation statements)

References 19 publications

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“…Consequently, HM-Cy and HM-OR are efficient and accurate in the determination of positive and negative results in the ELISA test. These results demonstrated the advantages of using recombinant CP as an antigen for antiserum production, as previously reported (Kim et al 1998;Li et al 1998;Cerovska et al 2002;Hélias et al 2003;Abou-Jawdah et al 2004).…”

Section: Discussionsupporting

confidence: 87%

“…This phenomenon may be due to the plant contaminants existing in purified virion preparations and therefore antibodies raised against virions react with the plant extracts in ELISA. Recently, a suitable alternative using recombinant CPs produced in bacterial cells instead of purified virions, was used to produce antiserum and proved to be very successful for detection purposes (Kim et al 1998;Li et al 1998;Cerovska et al 2002;Hélias et al 2003;Abou-Jawdah et al 2004).…”

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confidence: 99%

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Performances and application of antisera produced by recombinant capsid proteins of Cymbidium mosaic virus and Odontoglossum ringspot virus

Lee

Chang

2008

Eur J Plant Pathol

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Antisera against important orchid viruses, Cymbidium mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV), were separately produced using bacterially expressed recombinant capsid proteins (CP), instead of purified virus particles, as immunogens. These antisera were then designated as home-made CymMV CP antiserum (HM-Cy) and home-made ORSV CP antiserum (HM-OR). The high specificity of HM-Cy and HM-OR were confirmed by immunoblot. Their detection limits were determined using indirect-enzyme-linked immunosorbent assay (I-ELISA). Both HM-Cy and HM-OR showed low background reactivity to healthy plants and thus displayed a high S/H ratio (sample OD405/healthy control OD405) in tested orchids. The data indicated that our antisera were efficient and accurate in determination of negative and positive results in ELISA test as commercial antibodies. Therefore, these homemade antisera of CymMV and ORSV are suitable for the certification programme of orchids due to their low cost and high specificity. HM-Cy and HM-OR were further used for a field survey to study the incidence of CymMV and ORSV. The results showed that CymMV is more prevalent than ORSV in Taiwan.

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Section: Discussionsupporting

confidence: 87%

mentioning

confidence: 99%

Performances and application of antisera produced by recombinant capsid proteins of Cymbidium mosaic virus and Odontoglossum ringspot virus

Lee

Chang

2008

Eur J Plant Pathol

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“…Čeřovská et al 2007), and potato mop-top virus CP described by Helias et al (2003) who obtained only one serum reacting in DAS ELISA (which gave in comparison with commercial polyclonal antiserum 10× lower absorbance values).…”

Section: Discussionmentioning

confidence: 99%

Production of polyclonal antibodies to a recombinant coat protein of potato virus Y

et al. 2008

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The gene encoding the coat protein (CP) of a potato virus Y (PVY) was cloned into expression vector pMPM-A4Omega. PVY CP was expressed in Escherichia coli and the purified recombinant protein was used for raising rabbit polyclonal antibodies. The sera and antibodies were tested for the detection of PVY in the laboratory host Nicotiana tabacum cv. Petit Havana SR1 and in various cultivars of the natural host Solanum tuberosum by ELISA as well as by Western blots. The antibodies can be used for the detection of the whole strain spectrum of PVY by indirect plate trapped antigen ELISA and Western blot, but not by double antigen sandwich ELISA.

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“…Being a systemic pathogen PMTV prefers to reside in host cell cytosol (Tidona and Darai, 2002). It's usually found in low concentration and in uneven distribution in infected host tissues (Hélias et al, 2003). The genome of PMTV consists of three molecules of positive sense RNA species (+ssRNA) (Harrison and Reavy, 1974) (Fig 1).…”

mentioning

confidence: 99%

“…PMTV moves from cell to cell via RNA-TGB Molecule which encodes a triple gene block movement protein and also mini cysteine rich 8kD zinc finger protein. Coat protein (RNA-CP) is found to highly conserved among various isolates of PMTV (Hélias et al, 2003). For clearer picture, Genomic components of PMTV are shown in Table 1.…”

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confidence: 99%

A Review Paper on Potato Mop-Top Virus (Pmtv): Occurrence, Properties and Management

Abbas

Madadi

2016

World J. Biol. Biotechnol.

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Potato mop-top virus (PMTV) is a plant pathogenic virus that affects potatoes. The virus was initially reported from Germany but now it has spread throughout Europe, Asia, South America and North America. It is responsible for spraing symptoms (brown arcs/lines, blemishes, and rings) on potato tubers and yellow chevrons or mopping (Shortened internodes) in the leaves and stems of plants grown from infected potato tubers. PMTV causes huge economic losses due to poor tuber quality. It is an important disease in the potato growing areas of the world. PMTV is tubular rod shape and has a single stranded positive sense RNA (+ssRNA) tripartite genome. RNA 1 encodes RdRp (viral RNA-dependent RNA polymerase). Coat protein (20kDa) and a larger protein (91kDa) is encoded by RNA2. RNA2 encodes larger protein (91 kDa) by read through (RT) of the amber termination codon of the coat protein. There are three conserved moldular sets of genes known as triple gene block (TGB) which are coded by RNA3. These TGBs are involved in cell to cell or long distance movement of PMTV. In nature, PMTV is vectored and transmitted by a soil born pathogen (Plasmodiophorid (Spongospora subterranean f.sp. subterranean abbreviated as ‘Sss’) that itself causes the powdery scab disease on tubers. The disease caused by PMTV and Sss are favored by cool and damp conditions. PMTV remain in spore balls of Sss for several years even if the potato is not grown in the field. There are no efficient means to manage the virus nor its vector in an infested field, therefore, preventive measures are essential. Since PMTV along with its vector is causing important disease of potato, so understanding its molecular, biological, physical properties and management strategies is very important.

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Production of recombinant Potato mop-top virus coat protein in Escherichia coli and generation of antisera recognising native virus protein

Cited by 8 publications

References 19 publications

Performances and application of antisera produced by recombinant capsid proteins of Cymbidium mosaic virus and Odontoglossum ringspot virus

Performances and application of antisera produced by recombinant capsid proteins of Cymbidium mosaic virus and Odontoglossum ringspot virus

Production of polyclonal antibodies to a recombinant coat protein of potato virus Y

A Review Paper on Potato Mop-Top Virus (Pmtv): Occurrence, Properties and Management

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