Production of Renin by in vitro Cultures of Human Chorion and Uterine Muscle

Symonds, E. M.; Stanley, Margaret; Skinner, Sandford L.

doi:10.1038/2171152a0

Cited by 179 publications

(61 citation statements)

References 11 publications

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“…Hodari et al 18 and Symonds et al 19 were the first to convincingly report that renin is produced outside of the kidney; they verified renin production in placenta, chorion, and uterus, irrespective of preeclampsia status. Verification 20 in the gastrointestinal tract, 21 and in the brain, 22 were described later.…”

Section: Discussionmentioning

confidence: 99%

Dysregulation of the Circulating and Tissue-Based Renin-Angiotensin System in Preeclampsia

et al. 2007

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Abstract-The renin-angiotensin system (RAS) participates in preeclampsia; however, the relative contributions from the circulating RAS and the tissue-based, uteroplacental RAS are unknown. We hypothesized that the tissue-based uteroplacental RAS is dysregulated in preeclampsia. We performed microarray and gene expression studies and confirmed the findings on the protein level by immunohistochemistry in ureteroplacental units from 10 preeclamptic women and 10 women with uneventful pregnancies. All of the women were delivered by cesarean section. We also analyzed plasma renin activity and circulating agonistic angiotensin II type 1 (AT 1 ) receptor autoantibodies. In preeclampsia, we found that the angiotensin II AT 1 receptor gene was 5-fold upregulated in decidua (maternal origin). We also found AT 1 autoantibodies in preeclamptic women and in their offspring by neonatal cardiomyocyte bioassay compared with women with normal pregnancies and their infants (mother: 17.5Ϯ2.2 versus 0.05Ϯ0.4; fetus: 14.5Ϯ1.8 versus 0.5Ϯ0.5 ⌬bpm). Gene expressions for renin (35.0-fold), angiotensin-converting enzyme (2.9-fold), and angiotensinogen (8.9-fold) were higher in decidua than placenta (fetal origin) in both control and preeclamptic women, whereas the AT 1 receptor was expressed 10-fold higher in placenta than in decidua in both groups. Our findings elucidate the ureteroplacental unit RAS in preeclamptic and normal pregnancies. We found that, in preeclampsia, the AT 1 receptor expression is particularly high in decidua, combined with pregnancy-specific tissue RAS involving decidual angiotensin II production and AT 1 autoantibodies. We also showed that AT 1 autoantibodies cross the ureteroplacental barrier. These components could participate in the pathophysiology of preeclampsia.

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Section: Discussionmentioning

confidence: 99%

Dysregulation of the Circulating and Tissue-Based Renin-Angiotensin System in Preeclampsia

et al. 2007

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“…2 Gravid uterus in nephrectomized rabbits was shown through enzymatic assay for renin to be a source of renin. 12 Although it has been shown that human placenta expresses renin 13 and that chorionic cells secrete renin, mostly in the form of prorenin, 14,15 current evidence suggests that the placental prorenin may not enter the maternal circulation. 16 Therefore, renin production must occur on the maternal side for tissue renin to have a role in the local regulation of uterine blood flow.…”

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confidence: 99%

Definitive Molecular Evidence of Renin-Angiotensin System in Human Uterine Decidual Cells

Ansari

et al. 2000

Hypertension

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Abstract-The tissue renin-angiotensin system (RAS) has been suggested to be present in human gestational tissues, but uncertainty exists about the authenticity of this RAS, and the cellular origin of this RAS has not been defined. In the present study, we confirmed the presence of authentic renin and angiotensinogen in the prolactin-producing decidual tissue by sequencing the cDNAs generated through reverse transcription-polymerase chain reaction, confirming cDNA product sizes, and by performing Northern blot analysis of the RNA. Our comparative data demonstrate that prolactin has the highest expression in the decidual tissue, followed by renin, and that angiotensinogen has the least expression. We demonstrated with fluorescent in situ hybridization that prolactin-expressing endocrine decidual cells are the same cells that express both renin and angiotensinogen.

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“…Le placenta, le cordon ombilical, le chorion et l'amnios contiennent, non seulement de la rénine, mais aussi de l'angiotensine I et de l'angiotensine Il. Celle-ci est le composant le plus actif du système (Symonds et al, 1968;Poisner et al, 1981;Acker ef al, 1982;Egan et al, 1988;Pinet et al, 1988; Lenz et al, 1989).…”

Section: Introductionunclassified

Les récepteurs de l'angiotensine II dans le placenta humain sont de type AT₁

Kalenga

Gasparo²,

Hertogh³

et al. 1991

Reprod. Nutr. Dévelop.

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Production of Renin by in vitro Cultures of Human Chorion and Uterine Muscle

Cited by 179 publications

References 11 publications

Dysregulation of the Circulating and Tissue-Based Renin-Angiotensin System in Preeclampsia

Dysregulation of the Circulating and Tissue-Based Renin-Angiotensin System in Preeclampsia

Definitive Molecular Evidence of Renin-Angiotensin System in Human Uterine Decidual Cells

Les récepteurs de l'angiotensine II dans le placenta humain sont de type AT₁

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Production of Renin by in vitro Cultures of Human Chorion and Uterine Muscle

Cited by 179 publications

References 11 publications

Dysregulation of the Circulating and Tissue-Based Renin-Angiotensin System in Preeclampsia

Dysregulation of the Circulating and Tissue-Based Renin-Angiotensin System in Preeclampsia

Definitive Molecular Evidence of Renin-Angiotensin System in Human Uterine Decidual Cells

Les récepteurs de l'angiotensine II dans le placenta humain sont de type AT1

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Les récepteurs de l'angiotensine II dans le placenta humain sont de type AT₁