Production of Rumen-Protected Essential Amino Acids with Chemical Technique

Abstract

In this experiment, essential amino acid (Methionine) and two chemical compound, were used to make ligands that produce pH-sensitive amino acids that are stable in the rumen and absorbable in the post rumen part of the digestive tract. The treatments were made with heat and steer, reflux and ultrasound reaction at different times, temperatures and solvents to make new bonds and pH-sensitive amino acid ligands. During the first 8 hours of incubation (rumen phase) 55.42% of RPMet1 was released, during the 2 hours (abomasum phase) 91.00% of the compound had been released and during the final hours up to 35h (intestine phase) 93.21% had been released and 6.79% of this product was stable. About the RPMet2 figure shown that in first 8 hours of incubation (rumen phase) 49.25% of RPMet1 was released, in next 2 hours (abomasum phase) 87.93% of the compound was released and in the final hours up to 35h (intestine phase) 94.05% was released and 5.95% of this product was stable. Result shows that this chemical method increased retention time in rumen and the bond is reversible in lower pHs, similar to the abomasum.

Effects of rumen-bypass protein supplement on growth performance, hepatic mitochondrial protein complexes, and hepatic immune gene expression of beef steers with divergent residual feed intake

“…Ruminants mainly derive their amino acids from dietary protein that escapes rumen degradation and from microbial proteins [ 2 ]. Unlike other amino acids degraded in the rumen, rumen-bypass amino acids maintain their structural integrity, ensuring that they reach the bloodstream intact [ 3 , 4 ]. To optimize amino acids utilization in ruminants, introducing essential amino acids like lysine and methionine in forms that resist rumen degradation, but remain available for breakdown and absorption in the small intestine is advantageous [ 1 , 4 ].…”

Effects of rumen-bypass protein supplement on growth performance, hepatic mitochondrial protein complexes, and hepatic immune gene expression of beef steers with divergent residual feed intake

“…Ruminants mainly derive their amino acids (AA) from dietary protein that escapes rumen degradation and from microbial proteins [2]. Unlike other AAs degraded in the rumen, rumen-bypass amino acids maintain their structural integrity, ensuring that they reach the bloodstream intact [3,4]. To optimize AA utilization in ruminants, introducing essential AAs like lysine and methionine in forms that resist rumen degradation, but remain available for breakdown and absorption in the small intestine, is advantageous [1,4].…”

Feed additives in the diet of high-producing dairy cows

“…Although these products are not completely acceptable as their coated layers are still sensitive and could be damaged by chewing and physical or heat treatments used in diet preparation. On the other hand, most of the chemical methods could also be used for protecting amino acids as they do not have any physical layers so would not be damage by feed processing, therefore more work is needed to understand bioavailability of these products [Mazinani et al 2019].…”