Production of scopadulcic acid B from Scoparia dulcis Linn. using a Luffa sponge bioreactor

Mathew, Annie J.; Jayachandran, K.

doi:10.1007/s11240-009-9552-8

Cited by 9 publications

(2 citation statements)

References 23 publications

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“…Recently, largescale production of terpenoid scopadulcic acid B from Scoparia dulcis was reported using luffa sponge bioreactor (Mathew and Jayachandran 2009). Production of transgenic S. dulcis conferring herbicide resistance using Ri binary vector was the only documented transformation study (Yamazaki et al 1996).…”

Section: Discussionmentioning

confidence: 99%

Highly efficient production of transgenic Scoparia dulcis L. mediated by Agrobacterium tumefaciens: plant regeneration via shoot organogenesis

Mahender

Abbagani

Zhang

2011

Plant Biotechnol Rep

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Efficient Agrobacterium-mediated genetic transformation of Scoparia dulcis L. was developed using Agrobacterium tumefaciens strain LBA4404 harboring the binary vector pCAMBIA1301 with b-glucuronidase (GUS) (uidA) and hygromycin phosphotransferase (hpt) genes. Two-day precultured leaf segments of in vitro shoot culture were found to be suitable for cocultivation with the Agrobacterium strain, and acetosyringone was able to promote the transformation process. After selection on shoot organogenesis medium with appropriate concentrations of hygromycin and carbenicillin, adventitious shoots were developed on elongation medium by twice subculturing under the same selection scheme. The elongated hygromycin-resistant shoots were subsequently rooted on the MS medium supplemented with 1 mg l -1 indole-3-butyric acid and 15 mg l -1 hygromycin. Successful transformation was confirmed by PCR analysis using uidA-and hpt-specific primers and monitored by histochemical assay for b-GUS activity during shoot organogenesis. Integration of hpt gene into the genome of transgenic plants was also verified by Southern blot analysis. High transformation efficiency at a rate of 54.6% with an average of 3.9 ± 0.39 transgenic plantlets per explant was achieved in the present transformation system. It took only 2-3 months from seed germination to positive transformants transplanted to soil. Therefore, an efficient and fast genetic transformation system was developed for S. dulcis using an Agrobacteriummediated approach and plant regeneration via shoot organogenesis, which provides a useful platform for future genetic engineering studies in this medicinally important plant.

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Section: Discussionmentioning

confidence: 99%

Highly efficient production of transgenic Scoparia dulcis L. mediated by Agrobacterium tumefaciens: plant regeneration via shoot organogenesis

Mahender

Abbagani

Zhang

2011

Plant Biotechnol Rep

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“…To solve these problems, cell immobilization techniques have been applied to plant tissue culture so that unnecessary cell division is inhibited, and the immobilized cells gain characteristics of differentiated organs, which results in an increase in the production of secondary compounds (Mathew and Jayachandran 2009;Smetanska 2008;Tyler et al 1995). This technique involves physical anchorage of cells to a matrix, thus the cellular environment mimics a differentiated organ.…”

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confidence: 99%

Enhanced production and secretion of rutin and GABA in immobilized cells of mulberry tree (Morus bombycis K.)

Han

Lee

Song

et al. 2011

Plant Cell Tiss Organ Cult

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Cell immobilization has been proposed as a useful technique for mass production and efficient purification of secondary metabolites. In this study, we compared the bio-productivity of ligand-free and Ca-alginate-immobilized mulberry cells for rutin and c-amino butyric acid (GABA). In the leaves of Subong mulberry plants (M. bombycis K.) grown in a greenhouse, GABA accumulated as the leaves aged; a more than a 20-fold increase of GABA was observed in leaves undergoing senescence than in younger leaves. In contrast, more rutin was detected in mature leaves than in young leaves and those undergoing senescence. The production of total proteins in ligand-free leaf callus cells dramatically increased until 6 days after incubation in liquid suspension media (from 6.5 mg/g callus at day 0-14.5 mg/g callus), and by day 15 dropped to levels similar to those seen in the 0-day control. In contrast, immobilized cells showed a slight increase and then an insignificant decrease in protein content during the 15-day incubation period. Interestingly, immobilized mulberry cells more efficiently produced and secreted rutin and GABA into the suspension media than ligand-free cells. KN, a cytokinin, enhanced this production while 2,4-dichlorophenoxyacetic acid(2,4-D), an auxin, alleviated the effect of KN. As a result, incubation of the immobilized Subong cells in a full-strength Murashige and Skoog (MS) liquid medium containing 1 mg/l of 2,4-D and 0.1 mg/l KN, among the hormone combinations in the medium we tested, produced the highest amounts of rutin (8.2 lg/g callus cells) and GABA (305 lg/g callus cells) and secreted the largest amounts into the suspension media.

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pRi-Transformed Plants as a Source of Secondary Metabolites

2018

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Production of scopadulcic acid B from Scoparia dulcis Linn. using a Luffa sponge bioreactor

Cited by 9 publications

References 23 publications

Highly efficient production of transgenic Scoparia dulcis L. mediated by Agrobacterium tumefaciens: plant regeneration via shoot organogenesis

Highly efficient production of transgenic Scoparia dulcis L. mediated by Agrobacterium tumefaciens: plant regeneration via shoot organogenesis

Enhanced production and secretion of rutin and GABA in immobilized cells of mulberry tree (Morus bombycis K.)

pRi-Transformed Plants as a Source of Secondary Metabolites

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