Production of secondary metabolites in regenerated Southern wormwood (Artemisia abrotanum L.) under various experimental conditions

Alihodzic, Adna; Davis, Jimmy; Roberts, Christopher; Henrie, Sally; Bolyard, Mark

doi:10.1007/s11627-023-10382-0

Cited by 4 publications

(2 citation statements)

References 20 publications

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“…Furthermore, in vitro preservation is a source of tissues that are valuable for bioprospecting research [18], as well as the induction of mutagenesis for future applications. This biotechnological technique has several applications, including the study of physiological aspects [25], clonal propagation [26], the production of secondary metabolites [27], plant regeneration [28,29], and obtaining disease-free varieties [23,30,31].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Shoot Regeneration and Callogenesis of Sechium compositum (Donn. Sm.) C. Jeffrey for Plant Conservation and Secondary Metabolites Product

de la Luz,

Román,

Jorge

et al. 2024

Horticulturae

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Sechium compositum (Cucurbitaceae) is a wild species that is distributed in the Soconusco region, Chiapas, Mexico, and the border with Guatemala. This species has an intangible biochemical value resulting from the pharmacological relevance of its secondary metabolites. However, as a consequence of the lack of knowledge about its importance, it is being displaced from its habitat at an accelerated rate, incurring the risk of genetic loss. Therefore, an in vitro culture protocol with two experimental phases was evaluated to propagate, conserve, and regenerate this species. The first phases considered the shoot propagation, adding seven concentrations (0.1, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 mg mL−1) of 6-benzylaminopurine (BA) and thidiazuron (TDZ) and evaluating the number of buds and shoots and the shoot height. The best multiplication response was recorded with 0.1, 0.2, 0.4, and 1.0 mg L−1 of BA and 0.1 mg L−1 of TDZ, as well as the MS base culture medium. The validation of the results of the first phase (0.1 mg L−1 of BA) was compared with the MS in an independent experiment against the control (n = 50 repetitions), obtaining a height of 52 mm, 1.36 shoots, and 9.22 buds, suggesting that this concentration is adequate for the purpose, surpassing the MS control (MS culture medium alone). Of the total volume of roots obtained with packed bud structure in the previous experimental sample, it was reduced to 14% (n = 50). The second phase consisted of inducing callus formation from stem and leaf explants through the addition of 0.5, 1.0, and 2.0 mg L−1 of TDZ and 2,4-Dichlorophenoxyacetic acid (2,4-D) to the medium. Callus induction in S. compositum was better when using the stem in a medium with 2.0 mg L−1 of 2,4-D with a value of 97.8% around the explant. The addition of 500 mg L−1 of polyvinylpyrrolidone (PVP) is also suggested to reduce oxidation. This protocol represents a significant advance in the conservation, multiplication, and callus formation of S. compositum and contributes to its rescue and revaluation in the face of the danger of extinction.

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Section: Introductionmentioning

confidence: 99%

In Vitro Shoot Regeneration and Callogenesis of Sechium compositum (Donn. Sm.) C. Jeffrey for Plant Conservation and Secondary Metabolites Product

de la Luz,

Román,

Jorge

et al. 2024

Horticulturae

View full text Add to dashboard Cite

show abstract

“…Furthermore, in vitro preservation is a source of tissues that are valuable for bioprospecting research [18], as well as the induction of mutagenesis for future applications. This biotechnological technique has several applications, including the study of physiological aspects [25], clonal propagation [26], production of secondary metabolites [27] plant regeneration [28,29] and obtaining disease-free varieties [23,30,31].…”

Section: Introductionmentioning

confidence: 99%

Bud Multiplication and In Vitro Callogenesis of Sechium compositum (Donn. sm.) C. Jeffrey from Stem and Leaf Explants

Luz,

Román,

Mar

et al. 2023

Preprint

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Sechium compositum (Cucurbitaceae) is a wild species that is distributed in the Soconusco region, Chiapas, Mexico, and the border with Guatemala. This species has an intangible biochemical value resulting from the pharmacological relevance of its secondary metabolites. However, as a consequence of the lack of knowledge about its importance, it is being displaced from its habitat at an accelerated rate, incurring the risk of genetic loss. Therefore, an in vitro culture protocol with two experimental phases was evaluated to propagate, conserve, and regenerate this species. The first phases considered the multiplication of seedlings, adding seven concentrations (0.1, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 mg mL-1) of 6-benzylaminopurine (BA) and thidiazuron (TDZ) and evaluating the number of buds, shoots, and seedling height. The best multiplication response was recorded with 0.1, 0.2, 0.4, and 1.0 mg L-1 of BA and 0.1 mg L-1 of TDZ, as well as the MS base culture medium. With 0.1 mg L-1 of BA, 52-mm height, 1.36 shoots, and 9.22 buds were obtained, surpassing the MS control (MS culture medium alone); likewise, twice more roots (80%) and a 14% reduction in the bud structure were packed in 50 repetitions. The second phase consisted of inducing callus formation from stem and leaf explants through the addition of 0.5, 1.0, and 2.0 mg L-1 of TDZ and 2,4-Dichlorophenoxyacetic acid (2,4-D) to the medium. The results demonstrated that the induction of callogenesis in S. compositum has a better start from the stem in a medium with 2.0 mg L-1 of 2,4-D, whose value was 4.89 —equivalent to 97.8% callus formation around the explant. The addition of 500 mg L-1 of polyvinylpyrrolidone (PVP) is also suggested to reduce oxidation. This protocol represents a significant advance in the conservation, multiplication, and callogenesis of S. compositum and contributes to its rescue and revaluation in the face of the danger of extinction.

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Development of efficient, cost-effective in vitro micropropagation technique for threatened ethnomedicinal plant Clerodendrum indicum (L.) O. Kuntze

Kundu,

Sahani,

Adhikary

et al. 2024

Plant Cell Tiss Organ Cult

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Clerodendrum indicum (L.) O. Kuntze (Verbenaceae) is a threatened ethnomedicinal plant with many bioactive secondary metabolites that could alleviate chronic diseases like cough, asthma, jaundice, leprosy, syphilitic rheumatism, and septic wounds. Their natural growth has been severely challenged due to habitat loss and massive exploitation for medical applications, leading to the threatened status. Therefore, an in vitro micropropagation technique has been trialled to be used for eco-restoration and metabolite exploitation. Micropropagation via direct and indirect shoot organogenesis had been established from the different explants of this plant. Murashige and Skoog (MS) media supplemented with variable concentrations of NAA, IAA, and BAP produced callus, organogenesis and whole plant. The study revealed that nodal explants resulted in more signi cant responses than others. The shoot and root regeneration through callus was observed in the MS media supplemented with 4.0 mg/L 6-BA and 0.5 mg/L NAA with an 84% response rate after two weeks of incubation and an average 5.6 number of shoots per callus. Only root and shoot regeneration was observed using half-strength MS media with 2.0 mg/L 6-BA and 1.0 mg/L NAA and 6.0 mg/L 6-BA and 0.5 mg/L NAA, respectively, after two weeks. The plantlets acclimatization had an average of 80% survival rate. These in-vitro regenerated plants by direct shoot organogenesis and through callus induction methods might aid in harvesting a bulk amount of secondary metabolites without destroying the native habitat. Thus, the methods would lead to environmental restoration sustainably.

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Production of secondary metabolites in regenerated Southern wormwood (Artemisia abrotanum L.) under various experimental conditions

Cited by 4 publications

References 20 publications

In Vitro Shoot Regeneration and Callogenesis of Sechium compositum (Donn. Sm.) C. Jeffrey for Plant Conservation and Secondary Metabolites Product

In Vitro Shoot Regeneration and Callogenesis of Sechium compositum (Donn. Sm.) C. Jeffrey for Plant Conservation and Secondary Metabolites Product

Bud Multiplication and In Vitro Callogenesis of Sechium compositum (Donn. sm.) C. Jeffrey from Stem and Leaf Explants

Development of efficient, cost-effective in vitro micropropagation technique for threatened ethnomedicinal plant Clerodendrum indicum (L.) O. Kuntze

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