Production of selenium nanoparticles in Pseudomonas putida KT2440

Avendaño, Roberto; Chaves, Nefertiti; Fuentes, Paola; Sánchez, Ethel; Jiménez, José I.; Chavarría, Max

doi:10.1038/srep37155

Cited by 110 publications

(95 citation statements)

References 48 publications

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“…This method is considered a clean synthesis, but the cost and time of preparation of microbiological cultures is a disadvantage. [28][29][30] The use of plant extracts and biopolymers such as chitosan, konjac glucomannan, gum acacia, carboxymethyl cellulose, sodium alginate and glutathione, as reducing agents of selenite has also been an alternative, since they are highly biodegradable and non-toxic materials. However, in each of these works there are several processing steps until the attainment of SeNPs.…”

Section: Introductionmentioning

confidence: 99%

“Sweet Chemistry”: a Green Way for Obtaining Selenium Nanoparticles Active against Cancer Cells

Vieira¹,

Stein²,

Andreguetti³

et al. 2017

J. Braz. Chem. Soc.

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We present an environment friendly synthesis of selenium nanoparticles and the study of their cytotoxic activity against uterine sarcoma cancer and fibroblasts cells. Amorphous selenium (a-SeNPs) and trigonal selenium (t-SeNPs) were synthesized using D-fructose as the reducing agent and characterized by high-resolution transmission electron microscopy (HRTEM), energydispersive X-ray spectroscopy (EDX), powder X-ray diffraction analysis (XRD), inductively coupled plasma optical emission spectrometry (ICP OES), dynamic light scattering (DLS) to obtain zeta potential values and cyclic voltammetry (CV). Particularly, a-SeNPs presented high toxicity toward the resistant cancer cell line MES-SA/Dx5 and its parental MES-SA line. However, they are not toxic against P4 fibroblast cells in comparative studies. Keywords: selenium nanoparticles, D-fructose, cancer cells, green chemistry, cytotoxicity IntroductionSelenium is an essential element of significant importance not only for humans, but also for other forms of life. 1,2 Inorganic seleno-compounds have strong antioxidant activity, as well as some pro-oxidant effects and, additionally, have great importance in nutrition and medicine. 3,4 Epidemiological studies have already demonstrated the potential role of selenium in the prevention and treatment of cancer cells. [5][6][7] For example, selenium supplementation showed effective reduction in the incidence of cancer in the cervix, lung and liver. 8 However, the biological activity of elemental selenium stands opposite to the selenium salts, since selenite and selenate showed inadequate cytotoxicity to normal cells. [9][10][11] In this sense, nanoparticles of elemental selenium stand out as an alternative, due to its low toxicity and acceptable bioavailability. [12][13][14] Nanoparticles have become increasingly prominent in the medical field in the last few decades, giving rise to a new area, nanomedicine. This new area is focused on developing nanomaterials with potential use in therapy and diagnosis of diseases, [15][16][17] including selenium nanoparticles (SeNPs). 18Generally, the methods for obtaining SeNPs involve multiple steps, high temperatures and pressures; require expensive equipment and reagents, as well as being harmful to the environment. [19][20][21][22][23][24][25][26][27] In this sense, it is necessary to develop simple routes that focus on green chemistry and no harm to the environment. Research has demonstrated the use of microorganisms such as bacteria, to reduce selenite. This method is considered a clean synthesis, but the cost and time of preparation of microbiological cultures is a disadvantage. 28-30The use of plant extracts and biopolymers such as chitosan, konjac glucomannan, gum acacia, carboxymethyl cellulose, sodium alginate and glutathione, as reducing agents of selenite has also been an alternative, since they are highly biodegradable and non-toxic materials. However, in each of these works there are several processing steps "Sweet Chemistry": a Green Way for Obtaining Selenium Nanop...

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Section: Introductionmentioning

confidence: 99%

“Sweet Chemistry”: a Green Way for Obtaining Selenium Nanoparticles Active against Cancer Cells

Vieira¹,

Stein²,

Andreguetti³

et al. 2017

J. Braz. Chem. Soc.

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“…The efficiency of nanoparticle production by these halophilic strains was relativity higher than that reported previously, such as 89% and 70% efficiency of selenium nanoparticle synthesis by Pseudomonas putida (92.4% in this study) and Halococcous salifodinae (84.8% in this study), respectively [17,37]. The zeta potential indicates the degree of repulsion between adjacent, similarly charged particles in dispersion whose value can be related to the stability of colloidal dispersions [38].…”

Section: Plos Onementioning

confidence: 51%

“…Inductively coupled plasma-Optical emission spectrometry (ICP-OES). The efficiency of the production of nanoparticles was determined based on the measurements of the remaining amount of primary silver and selenite salts in the culture media/reaction mixtures by the inductively coupled plasma optical emission spectrometry (ICP-OES) (SPECTRO ARCOS, Germany) [17].…”

Section: Characterization Of Nanoparticlesmentioning

confidence: 99%

Exploring the potentials of halophilic prokaryotes from a solar saltern for synthesizing nanoparticles: The case of silver and selenium

2020

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Halophiles are the organisms that thrive in extreme high salt environments. Despite the extensive studies on their biotechnological potentials, the ability of halophilic prokaryotes for the synthesis of nanoparticles has remained understudied. In this study, the archaeal and bacterial halophiles from a solar saltern were investigated for the intracellular/extracellular synthesis of silver and selenium nanoparticles. Silver nanoparticles were produced by the archaeal Haloferax sp. (AgNP-A, intracellular) and the bacterial Halomonas sp. (AgNP-B, extracellular), while the intracellular selenium nanoparticles were produced by the archaeal Halogeometricum sp. (SeNP-A) and the bacterial Bacillus sp. (SeNP-B). The nanoparticles were characterized by various techniques including UV-Vis spectroscopy, XRD, DLS, ICP-OES, Zeta potentials, FTIR, EDX, SEM, and TEM. The average particle size of AgNP-A and AgNP-B was 26.34 nm and 22 nm based on TEM analysis. Also, the characteristic Bragg peaks of face-centered cubic with crystallite domain sizes of 13.01 nm and 6.13 nm were observed in XRD analysis, respectively. Crystallographic characterization of SeNP-A and SeNP-B strains showed a hexagonal crystallite structure with domain sizes of 30.63 nm and 29.48 nm and average sizes of 111.6 nm and 141.6 nm according to TEM analysis, respectively. The polydispersity index of AgNP-A, AgNP-B, SeNP-A, and SeNP-B was determined as 0.26, 0.28, 0.27, and 0.36 and revealed high uniformity of the nanoparticles. All of the synthesized nanoparticles were stable and their zeta potentials were calculated as (mV):˗33.12, ˗35.9, ˗31.2, and ˗29.34 for AgNP-A, AgNP-B, SeNP-A, and SeNP-B, respectively. The nanoparticles showed the antibacterial activity against various bacterial pathogens.

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“…GSHR was responsible for reducing selenite and tellurite to insoluble NP's using the O-2 strain of Pseudomonas maltophilia; 39 GSHR and thioredoxin reductase are responsible for selenite and selenate reduction in Pseudomonas seleniipraecipitansI. [40][41][42][43] The highly conserved sequence across species within the Pseudomonas genus, including conservation in the product/substrate binding pocket, is suggestive that the ability to handle normally toxic amounts of SeO 3 2-may be a general feature of the Pseudomonas genus. This Se tolerance may arise from the nature of GSHRs in this genus.…”

Section: Resultsmentioning

confidence: 99%

The Metalloid Reductase of Pseudomonas Moravenis Stanleyae Conveys Nanoparticle Mediated Metalloid Tolerance

Nemeth

Neubert²,

Ni³

et al. 2018

Preprint

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When cultured independently in liquid media, it tolerates SeO 3 2-supplementation in liquid culture up to 10mM. This is 10-fold more than the SeO 3 2-tolerance of most other microbes. We attributed the observed selenite reduction activity of P. moravenis to a GSHR-like enzyme on the basis of proteomic mass spectrometry of an in-gel in situ selenium reductase activity.GSHRs generally belong to the family of pyridine nucleoside dependent oxidoreductases.This enzyme family also, notably, includes another well-characterized metal reducing enzyme -- 4In prior study, we characterized commercially sourced Saccharomyces Cervisiae (S. Cervisiae) GSHR for selenite reductase activity, showing the ability of the enzyme to oxidize NADPH while reducing SeO 3 2-to Se(0) nanoparticles. 13 In the present study, we characterize a homologous metalloid reductase from the seleno-specialist P. moravenis. We find that the substrate selectivity of the metalloid reductase (K M ) shows a substantially larger preference for GS-Se-SG relative to all other reported glutathione reductase enzymes. These enzymatic properties can be partially rationalized in terms of sequence and corresponding homologymodeled structure of the enzyme. We also observe that expressing this enzyme in laboratory strains of E. Coli (BL21, SS320) results in increased tolerance to SeO 3 2-, as well as the presence of Se nanoparticles in these cells. Overall, our data suggests that the enzyme may be best described as a glutathione reductase-like-metalloid reductase (GRLMR). RESULTS AND DISCUSSIONAltered substrate specificity of GRLMR enzymes, favoring selenodiglutathione (GS-Se-SG) over oxidized glutathione (GSSG) as a substrate could underlie the remarkable SeO 3 2-tolerance of P. Moravenis stanleyae. We therefore characterized the P. Moravenis stanleyae GRLMR enzyme identified previously. The DNA sequence of the enzyme was acquired through a fullgenome sequencing (ACGT Inc,Wheeling, IL). Sequencing was conducted using de novo paired end sequencing. 21 This revealed a genome where 70.3% of the nucleobases have, at the most, a 1:1000 probability of mis-assignment. Figure S1 shows the "Quality Score" (Q score) for each sequenced base with Q=-log10(e). Q scores, are derived from a phred-like error probability assessment of each individual nucleotide. 5A BLAST search of the genomic sequence, using the Pseudomonas R-28S GSHR as a reference, identified one GSHR-like sequence, with 93% sequence homology. Sequence alignment of this GRLMR DNA using Serial Cloner show high similarity (98.00%) toPseudomonas fluorescines (P. fluorescines) GSHR and modest similarity (67 -71%) to E. coli, S. cervisiae, and Homo sapiens (H. sapiens) GSHR DNA. The sequence similarities are summarized in Table 1, and full alignments are shown in the supporting information, Figure S2.The DNA sequence, combined with homology modeling of the structure suggest that all of the

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Production of selenium nanoparticles in Pseudomonas putida KT2440

Cited by 110 publications

References 48 publications

“Sweet Chemistry”: a Green Way for Obtaining Selenium Nanoparticles Active against Cancer Cells

“Sweet Chemistry”: a Green Way for Obtaining Selenium Nanoparticles Active against Cancer Cells

Exploring the potentials of halophilic prokaryotes from a solar saltern for synthesizing nanoparticles: The case of silver and selenium

The Metalloid Reductase of Pseudomonas Moravenis Stanleyae Conveys Nanoparticle Mediated Metalloid Tolerance

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