Production of shikonin derivatives by cell suspension cultures of Lithospermum erythrorhizon

Abstract

An excellent new medium was developed for the production of shikonin derivatives by suspension cultures of Lithospermum erythrorhizon. We investigated the effects of all the components of White's medium on the production of these derivatives. Nitrate, phosphate, copper, sulfate and sucrose had especially marked effects. With the new, M-9, medium produced from these studies the yield of shikonin derivatives was 1400 mg/l and the yield for dried cells was about 12%, whereas it was 120 mg/l, or about 2% with White's medium.

Regulation of Lithospermic Acid B and Shikonin Production in Lithospermum erythrorhizon Cell Suspension Cultures.

“…34). In particular, shikonin production is clearly enhanced by the supplementation of Cu 2ϩ , 35) but strongly inhibited by either NH 4 ϩ 25,36) or 2,4-D. 37) We examined the effects of such shikonin production modulators on LAB production. As shown in tion in M-9 medium in a dose-dependent manner.…”

“…Cellsuspension cultures in 100-ml flasks each containing 30 ml of medium were agitated on a rotary shaker (80 rpm) at 23°C in the dark and subcultured at intervals of 2 weeks. The cells cultured in LS medium for 2 weeks were inoculated into M-9 medium, 25) modified M-9 or LS media containing 3% sucrose, and the same growth regulators as above (inoculum size: 1 g of fresh cells/30 ml of medium in a 100-ml flask) and cultured for 3 weeks under the above-mentioned culture conditions. For the investigation of the effect of light color on the production of secondary metabolites, each flask was covered with a commercially available colored cellophane sheet, 28) and irradiated with white light (10000 lx) from fluorescent lamps during the entire culture period.…”

Enhancement of Shikalkin Production in Arnebia euchroma Callus by a Fungal Elicitor, Rhizoctonia solani

“…A. euchroma specimens were collected and determined as described earlier (9). Seed germination and callus induction were successfully carried out according to the reported method (14). The resulting calli from leaf, collar and root explants, obtained from young plantlets, were propagated first on MS medium supplemented with sucrose (50 g.L -1 ), 2,4-D (10 -6 M), and kinetin (10 -5 M) during three successive subcultures at 25°C in darkness.…”

“…In the second step, the cells are transferred to a production medium such as White medium containing IAA and kinetin. The whole process is carried out in the darkness at 25°C (12)(13)(14)(15).…”

Glutathione Enhanced Anthraquinone Production in Advntitious Root Cultures of Rubia tinctorum L.

“…Fujita et al reported that Cu ions had marked effects on the production of shikonin derivatives by suspension cultures of Lithospermum erytrorhizon [3]. Christen et al, reported that Cu2+ remarkably enhanced both the growth and the alkaloid yield in hairy root cultures of Hyoscyamus albus [4].…”