Production of small RNAs by mammalian Dicer

Svobodová, Eliška; Kubíková, Jana; Svoboda, Petr

doi:10.1007/s00424-016-1817-6

Cited by 47 publications

(40 citation statements)

References 121 publications

(213 reference statements)

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“…Dicer, a key component of the microRNA processing machinery, has been reported to exert discrepant prognostic values and biological roles in different types of cancers [6, 17–21]. In this present study, we investigated the precise function and prognostic value of Dicer in ccRCC.…”

Section: Discussionmentioning

confidence: 92%

Dicer suppresses MMP-2-mediated invasion and VEGFA-induced angiogenesis and serves as a promising prognostic biomarker in human clear cell renal cell carcinoma

Chen

Meng

et al. 2016

Oncotarget

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Dicer, a key component of the microRNA processing machinery, has been reported to exert discrepant prognostic values and biological roles in different types of cancers. Here, we investigated the function and prognostic value of Dicer in clear cell renal cell carcinoma (ccRCC). Using the retrospective ccRCC patients’ cohorts with tissue microarray (TMA), we demonstrated that Dicer expression was significantly down-regulated in ccRCC compared with renal non-tumor tissues, and negatively associated with pN status (P = 0.005), pM status (P = 0.009) and TNM stage (P =0.013). Multivariate Cox proportional hazards regression analyses showed that positive Dicer expression was an independent favorable factor for prognosis of ccRCC patients (hazard ratio (HR) = 0.709, P = 0.025 for 5-year overall survival; HR = 0.655, P = 0.008 for disease specific survival). Moreover, we found that Dicer decreased the abilities of cell migration, invasion and angiogenesis through suppressing MMP-2 and VEGFA expression. Tumor metastasis model in vivo showed much more metastatic nodules of lung in the Dicer knockdown group than the control group via increased MMP-2 expression. Our findings imply that Dicer inhibits ccRCC metastasis and may serve as promising prognostic biomarkers for ccRCC patients.

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Section: Discussionmentioning

confidence: 92%

Dicer suppresses MMP-2-mediated invasion and VEGFA-induced angiogenesis and serves as a promising prognostic biomarker in human clear cell renal cell carcinoma

Chen

Meng

et al. 2016

Oncotarget

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“…2 and 7A). DICER1 participates primarily in the biogenesis of (multiple classes of) small RNAs, including miRNAs (56). However, other functions have been attributed to this complex that include the processing of tRNAs and exogenous RNAs (57).…”

Section: Resultsmentioning

confidence: 99%

Exploring the Human-Nipah Virus Protein-Protein Interactome

Martínez-Gil

Vera-Velasco

Mingarro

2017

J Virol

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Nipah virus is an emerging, highly pathogenic, zoonotic virus of the family. Human transmission occurs by close contact with infected animals, the consumption of contaminated food, or, occasionally, via other infected individuals. Currently, we lack therapeutic or prophylactic treatments for Nipah virus. To develop these agents we must now improve our understanding of the host-virus interactions that underpin a productive infection. This aim led us to perform the present work, in which we identified 101 human-Nipah virus protein-protein interactions (PPIs), most of which (88) are novel. This data set provides a comprehensive view of the host complexes that are manipulated by viral proteins. Host targets include the PRP19 complex and the microRNA (miRNA) processing machinery. Furthermore, we explored the biologic consequences of the interaction with the PRP19 complex and found that the Nipah virus W protein is capable of altering p53 control and gene expression. We anticipate that these data will help in guiding the development of novel interventional strategies to counter this emerging viral threat. Nipah virus is a recently discovered virus that infects a wide range of mammals, including humans. Since its discovery there have been yearly outbreaks, and in some of them the mortality rate has reached 100% of the confirmed cases. However, the study of Nipah virus has been largely neglected, and currently we lack treatments for this infection. To develop these agents we must now improve our understanding of the host-virus interactions that underpin a productive infection. In the present work, we identified 101 human-Nipah virus protein-protein interactions using an affinity purification approach coupled with mass spectrometry. Additionally, we explored the cellular consequences of some of these interactions. Globally, this data set offers a comprehensive and detailed view of the host machinery's contribution to the Nipah virus's life cycle. Furthermore, our data present a large number of putative drug targets that could be exploited for the treatment of this infection.

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“…In fact, within the coding sequence for the third α-helix there is a 20-bp stretch with 100% nucleotide identity (Fig. 1C), which is roughly the effective length of sequence for achieving systemic knockdown by RNA interference (RNAi) 28 . Indeed, Tc-zen1-specific double-stranded RNA (dsRNA) that spans the homeobox is sufficient to effect significant cross-paralogue knockdown of Tc-zen2 ( Fig.…”

Section: Recent Tandem Duplication Of Zen In the Tribolium Lineagementioning

confidence: 99%

Unexpected mutual regulation underlies paralogue functional diversification and promotes maturation of a protective epithelial tissue

Gurská

2018

Preprint

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Insect Hox3/zen genes represent an evolutionary hotspot for changes in function and copy number. Single orthologues are required either for early specification or late morphogenesis of the extraembryonic tissues, which protect the embryo. The tandemly duplicated zen paralogues of the beetle Tribolium castaneum present a unique opportunity to investigate both functions in a single species. We dissect the paralogues' expression dynamics (transcript and protein) and transcriptional targets (RNA-seq after RNAi) throughout embryogenesis. We identify an unexpected role of Tc-Zen2 in repression of Tc-zen1, generating a negative feedback loop that promotes developmental progression. Tc-Zen2 regulation is dynamic, including within co-expressed multigene loci. We also show that extraembryonic development is the major event within the transcriptional landscape of late embryogenesis and provide a global molecular characterization of the extraembryonic serosal tissue. Altogether, we propose that paralogue mutual regulation arose progressively and drove multiple instances of zen subfunctionalization, leading to complementary extant roles.

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Production of small RNAs by mammalian Dicer

Cited by 47 publications

References 121 publications

Dicer suppresses MMP-2-mediated invasion and VEGFA-induced angiogenesis and serves as a promising prognostic biomarker in human clear cell renal cell carcinoma

Dicer suppresses MMP-2-mediated invasion and VEGFA-induced angiogenesis and serves as a promising prognostic biomarker in human clear cell renal cell carcinoma

Exploring the Human-Nipah Virus Protein-Protein Interactome

Unexpected mutual regulation underlies paralogue functional diversification and promotes maturation of a protective epithelial tissue

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