Production of somatic embryos and shoots from sugarbeet callus: Effects of abscisic acid, other growth regulators, nitrogen source, sucrose concentration and genotype

Saunders, J. W.; Tsai, C. J.

doi:10.1007/s11627-999-0004-2

Cited by 16 publications

(4 citation statements)

References 26 publications

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“…On the other hand, sucrose has been used as the major carbon source in tissue culture (Fuentes et al 2000;Yang et al 2006), which has been found to affect somatic embryogenesis in many plant species (Karami et al 2006;Lou and Kako 1995;Saunders and Tsai 1999). In this study, the effect of sucrose pretreatment on increased embryogenic potential may be linked to both an increase in carbon supply and a change in osmotic potential.…”

Section: Discussionmentioning

confidence: 88%

Enhancement of somatic embryogenesis in camphor tree (Cinnamomum camphora L.): osmotic stress and other factors affecting somatic embryo formation on hormone-free medium

Shi

Dai

et al. 2009

Trees

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The aim of this study was to improve the direct somatic embryogenesis and initiate embryogenic callus formation in camphor tree (Cinnamomum camphora L.) on hormone-free medium. The influence of osmotic stress pretreatment of immature zygotic embryos (0.5 and 1.0 M solution of sucrose for 12, 24, 48, 72, 96, 120, and 144 h at 4 or 25°C) before cultured on hormone-free medium, on embryogenesis efficiency was assessed. The embryogenesis frequency was improved from 16.29 to 93.27%, while the average number of somatic embryos per explant increased from 3 to 12.57. Activated charcoal (AC), medium renewal, basal medium, light conditions and sucrose concentration in culture medium were also evaluated for their effect on somatic embryogenesis. AC addition and 10-day medium renewal did not increase embryogenesis efficiency significantly, and Murashige and Skoog (MS) medium proved to be more beneficial for somatic embryo formation than others. No differences were found between embryogenesis frequencies when cultured in darkness or under light, but culturing under light yielded more embryos. After the sucrose solution pretreatment, high level concentration of sucrose in induction medium was not needed for somatic embryogenesis, for it had a negative effect on somatic embryo formation when the concentration of sucrose was higher than 50 g l -1 . The derived embryogenic lines were maintained via repetitive embryogenesis on hormone-free medium. Low ratio formation of embryogenic callus was observed on the surface of somatic embryos both on induction and proliferation medium. Plantlets derived from somatic embryos grew vigorously with normal appearance similar to germinated zygotic embryos.

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Section: Discussionmentioning

confidence: 88%

Enhancement of somatic embryogenesis in camphor tree (Cinnamomum camphora L.): osmotic stress and other factors affecting somatic embryo formation on hormone-free medium

Shi

Dai

et al. 2009

Trees

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“…2). Genotypic variation, primarily because of its highly heterozygous nature due to outcrossing, is a serious problem for in vitro regeneration of sugar beet as certain genotypes are more amenable than others to organogenesis (Detrez et al 1988;Zhong et al 1993b;Gurel et al 2001;Mishutkina and Gaponenko 2006) and somatic embryogenesis (Saunders and Tsai 1999;Zhang et al 2008). To use large numbers of explant replicates from many individual plants within a single line/population was suggested as a mean to cope with variability in sugar beet (Doley and Saunders 1989;IvicHaymes and Smigocki 2005) but the laboriousness of plant tissue culture, coupled with the need to standardize production of parental plants, often restricts feasibility of this approach.…”

Section: Discussionmentioning

confidence: 99%

A two-stage pretreatment of seedlings improves adventitious shoot regeneration in sugar beet (Beta vulgaris L.)

Gürel

Baloğlu

Gürel

et al. 2011

Plant Cell Tiss Organ Cult

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The effects of a two-stage pretreatment of seedlings on the subsequent shoot regeneration capacity were investigated. Pretreated seedlings were obtained by germinating seeds on three different germination media and then further culturing on six different growth media. Lamina and petiole explants of two sugar beet (Beta vulgaris L.) breeding lines were then excised from the pretreated seedlings and cultured on five different shoot regeneration media. In both breeding lines, petiole explants produced significantly more shoots than lamina explants with higher frequencies of organogenic capacities; petiole explants of the lines M1195 and ELK345 produced a mean of 2.1 and 2.7 shoots per explant while their lamina explants produced 1.5 and 2.2 shoots per explant, respectively. A genotypic variation was evident as the line ELK345 was more productive for shoot development from both types of explants. In overall comparisons of different germination, growth and regeneration media, germination medium was most effective when supplemented with 0.5 mg/l 6-benzyladenine (BA) while both growth and regeneration media were most productive when contained a combination of 0.25 mg/l BA and 0.10 mg/l indole-3-butyric acid (IBA). Of all the treatments tested, the highest mean number of shoots per explant (8.3 shoots) and frequency of organogenic explants (75.6%) were obtained on regeneration medium supplemented with 0.25 mg/l BA and 0.10 mg/l IBA when petiole explants of the line ELK345 were excised from the seedlings that had been germinated on medium containing 0.5 mg/l BA followed by further growth on medium containing 0.25 mg/l BA and 0.10 mg/l IBA.

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“…The application of these compounds 146 BENSON (frequently in combination with ABA) can greatly improve recalcitrance problems associated with embryo and organogenic development in vitro and whole plant regeneration. Thus, ABA and optimal sucrose levels increased the production of somatic embryos and shoots in sugar beet callus (Saunders and Tsai, 1999) and polyethylene glycol combined with ABA induced the later stages of embryo development in Pinus taeda genotypes which were previously unresponsive (Li et al, 1997).…”

Section: In Vitro Manipulationsmentioning

confidence: 90%

Sepecial symposium: In vitro plant recalcitrance in vitro plant recalcitrance: An introduction

Benson

2000

In Vitro Cell.Dev.Biol.-Plant

139

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In vitro recalcitrance is the inability of plant cells, tissues and organs to respond to tissue culture manipulations. With respect to plant regeneration, recalcitrance can be a major limiting factor for the biotechnological exploitation of economically important plant species and it can also impair the wider application of in vitro conservation techniques. This first paper introduces a compilation of Symposium papers, collectively entitled ''Do we understand in vitro plant recalcitrance?'', presented at the 1999 Congress of the Society for In Vitro Biology. The Symposium reviewed recalcitrance in the context of genetic predeterminism, molecular markers and gene expression patterns, whole and explant physiology, stress physiology, habituation, neoplastic progression and plant cancer. The symposium contributors present fundamental and applied investigative approaches which have the potential to enhance our current understanding of in vitro recalcitrance and to assist in overcoming the problems associated with nonresponsive plant cultures. This introductory paper presents the general concept of recalcitrance in relation to whole-plant and explant physiology and considers basic aspects of tissue culture manipulations in the context of recalcitrance problems.

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Production of somatic embryos and shoots from sugarbeet callus: Effects of abscisic acid, other growth regulators, nitrogen source, sucrose concentration and genotype

Cited by 16 publications

References 26 publications

Enhancement of somatic embryogenesis in camphor tree (Cinnamomum camphora L.): osmotic stress and other factors affecting somatic embryo formation on hormone-free medium

Enhancement of somatic embryogenesis in camphor tree (Cinnamomum camphora L.): osmotic stress and other factors affecting somatic embryo formation on hormone-free medium

A two-stage pretreatment of seedlings improves adventitious shoot regeneration in sugar beet (Beta vulgaris L.)

Sepecial symposium: In vitro plant recalcitrance in vitro plant recalcitrance: An introduction

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