Production of some biologically active secondary metabolites from marine-derived fungus Varicosporina ramulosa

Abstract

In a screening of fungal isolates associated with marine algae collected from Abou-keer, Alexanderia during the four seasons of 2004, to obtain new biologically active compounds. Varicosporina ramulosa isolate was identified and selected as a producer of 13 compounds. Out of 13 pure compounds produced, compounds 3 and 10 were considered as antibacterial and antifungal compounds, respectively as they were active against gram positive, gram negative bacteria and a fungus. Optimization of conditions (fermentation media, incubation period, temperature, initial pH, aeration levels) which activate compounds 3 and 10 production were studied. Also the spectral properties (UV, MS, GC/MS, IR and 1 H-NMR) of the purified compounds were determined. Compound 3 suggested to be dibutyl phthalate and compound 10 may be ergosterol or one of its isomers. Biological evaluation of the two compounds towards 6 different types of tumor cell lines showed weak effect of compound 3 at different concentrations on the viable cell count of the different tumor cell lines. While compound 10 showed different activities against the viable cell count of the 6 different tumor cell lines. It kills 50% of the viable infected liver and lung cells at concentrations equal to 99.7 µg/mL, 74.9µg/mL, respectively. Compound 10 can be recommended as new anticancer compounds.

The Effect of Modified Media on The Antibacterial Activity of The Sea Sponge Symbion Fungi, Fusarium solani

“…Keuntungan dari jamur relatif lebih mudah dikembangbiakkan, tidak menghabiskan banyak tempat serta pertumbuhannya cepat. Produksi metabolit sekunder ditentukan oleh jenis jamur, kondisi lingkungan tempat tumbuh jamur (Zhang et al, 2008), dan juga dipengaruhi oleh media dan kondisi pertumbuhan seperti lamanya waktu inkubasi, suhu, pH, dan tingkat aerasi (Atalla et al, 2008).…”