Production of Sophorolipid Glycolipid Biosurfactants from Sugarcane Molasses Using &lt;i&gt;Starmerella bombicola&lt;/i&gt; NBRC 10243

Takahashi, Makoto; Morita, Tomotake; Wada, Kōji; Hirose, Naoto; Fukuoka, Tokuma; Imura, Tomohiro; Kitamoto, Daï

doi:10.5650/jos.60.267

Cited by 62 publications

(23 citation statements)

References 25 publications

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“…Biosurfaktan merupakan salah satu sumber energi alternatif yang disintesis secara ekstraselular oleh mikroba dengan aktivitas sebagai penurun tegangan permukaan (Takahashi et al 2011). Berdasarkan struktur, molekul surfaktan mengandung gugus hidrofilik dan hidrofobik, yaitu suatu sifat yang mampu mengkonsentrasikan molekul-molekul interpermukaan yang berbeda derajat polaritasnya, seperti interpermukaan minyak dalam air (Rau et al 2005).…”

Section: Pendahuluanunclassified

Potensi bakteri lumpur minyak sebagai penghasil biosurfaktan dan antimikroba

Sari

Afiati

Kusharyoto

2015

Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia

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Senyawa glikolipid yang diproduksi oleh bakteri lumpur minyak memiliki potensi sebagai biosurfaktan dan agen antimikrobial. Untuk mengkonfirmasi kemampuan isolat dalam menghasilkan senyawa biosurfaktan, dilakukan uji pendahuluan hemolisis dalam cawan agar darah (blood agar plate). Selanjutnya kelima bakteri ditumbuhkan dalam medium YM menggunakan minyak zaitun sebagai stimulan terhadap keluarnya ekstraseluler surfaktan (biosurfaktan). Uji kualitatif senyawa biosurfaktan diobservasi menggunakan kromatografi lapis tipis. Penelitian ini bertujuan untuk menguji potensi ekstrak bakteri lumpur minyak sebagai penghasil senyawa biosurfaktan dan aktivitas antibakterial. Hasil penelitian menunjukkan bahwa identitas kimia senyawa bioaktif surfaktan terdeteksi positif dalam bentuk spot kuning di pelat TLC dan menunjukkan reaksi positif dengan pewarna antron. Seluruh ekstrak bakteri lumpur minyak berpotensi sebagai senyawa biosurfaktan dan mampu menekan pertumbuhan mikroba patogen berupa Bacillus subtilis, Escherichia coli, dan Staphylococcus aureus.

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Section: Pendahuluanunclassified

Potensi bakteri lumpur minyak sebagai penghasil biosurfaktan dan antimikroba

Sari

Afiati

Kusharyoto

2015

Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia

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“…The cultures were centrifuged for 15 min at 10,000 × g to obtain cell-free supernatant, after subjected to the liquid-liquid extraction for isolation of procedure biosurfactant using different organic solvents such as chloroform/methanol (3:2:1), acetone (1:1), ethyl acetate (1:1) and ethanol (2:1) [14][15][16].…”

Section: Biosurfactant Isolation and Characterization By Spectrometermentioning

confidence: 99%

Optimization and Evaluation of Biosurfactant Produced by Pantoea sp. Using Pineapple Peel Residue, Vegetable Fat and Corn Steep Liquor

Almeida¹,

Silva²,

Garrard³

et al. 2015

JCCE

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Abstract:In this study, the authors have investigated the potential of a bacterial strain of Pantoea sp., isolated from wastewater of the textile industry, for the production of biosurfactant. The biosurfactant production was optimized by the combination of CCD (central composite design) and RSM (response surface methodology). To assess the effects and interactions of medium the vegetable fat (1.5, 2.0 and 2.5 v/v), the variables corn steep liquor (2.0, 5.0 and 8.0 v/v) and pineapple peel residue (10.0, 25.0 and 40.0 v/v) on the surface tension were evaluated. The empirical model developed through RSM in terms of the effective operational factors mentioned above was found to be adequate to describe the biosurfactant production. Compositional analysis of the produced biosurfactant has been carried out by FT-IR (Fourier transform infrared spectoscopy) and subjected to the test of removing hydrocarbons. Through the analysis, vegetable fat and pineapple peel residue were found to be the most significant factors, whereas corn steep liquor had less effect within the ranges investigated. A maximum reduction in surface tension of 30.00 mN/m was obtained under the optimal conditions of 2.0% (v/v) vegetable fat concentration, 5.0% (v/v) corn steep liquor and 25.0% (v/v) pineapple peel residue concentration of medium. FT-IR spectrometer analysis of the biosurfactant characterized it as a glycolipid derivative. The biosurfactant exhibited the ability to solubilize the hydrocarbons tested, working between 64% and 92%. According to consists of bars with a length proportional to the absolute value of the estimated effects divided by the standard error. On this chart, ANOVA (analysis of variance) effect estimates are arranged from the largest to smallest absolute value. The chart includes a vertical line at the critical p-value of 0.05. Effects for which the bars are smaller than the critical p-value are considered non-significant and do not have an effect on the response variables. The effects are either positive or negative ANOVA; the determination of regression coefficients and the construction of graphs were performed using the Statistical ® program, version 7.0 (Statsoft Inc, RSA). The results, the biosurfactant produced by Pantoea sp. can be a valuable source for application in rapid environmental bioremediation.

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“…Recent studies on SLs have focused on utilizing cheap substrates (Shah et al, 2007;Solaiman et al, 2014) and improving production efficiency (Daverey and Pakshirajan, 2009). Several substrates were explored for replacing glucose in the SL fermentation medium, such as glycerol (Bhangale et al, 2014), sugarcane molasses (Takahashi et al, 2011) and corncob hydrolysate (Konishi et al, 2015). Maddikeri et al (2015) produced 55.6 g l À1 SLs from waste frying oil using ultrasound technology.…”

Section: Introductionmentioning

confidence: 99%

Semicontinuous sophorolipid fermentation using a novel bioreactor with dual ventilation pipes and dual sieve‐plates coupled with a novel separation system

Zhang

Dan

Sun

et al. 2017

Microbial Biotechnology

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SummarySophorolipids (SLs) are biosurfactants with widespread applications. The yield and purity of SLs are two important factors to be considered during their commercial large‐scale production. Notably, SL accumulation causes an increase in viscosity, decrease in dissolved oxygen and product inhibition in the fermentation medium. This inhibits the further production and purification of SLs. This describes the development of a novel integrated system for SL production using Candida albicans O‐13‐1. Semicontinuous fermentation was performed using a novel bioreactor with dual ventilation pipes and dual sieve‐plates (DVDSB). SLs were separated and recovered using a newly designed two‐stage separation system. After SL recovery, the fermentation broth containing residual glucose and oleic acid was recycled back into the bioreactor. This novel approach considerably alleviated the problem of product inhibition and accelerated the rate of substrate utilization. Production of SLs achieved was 477 g l−1, while their productivity was 1.59 g l−1 h−1. Purity of SLs improved by 23.3%, from 60% to 74%, using DVDSB with the separation system. The conversion rate of carbon source increased from 0.5 g g−1 (in the batch fermentation) to 0.6 g g−1. These results indicated that the integrated system could improve the efficiency of production and purity of SLs.

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Production of Sophorolipid Glycolipid Biosurfactants from Sugarcane Molasses Using <i>Starmerella bombicola</i> NBRC 10243

Cited by 62 publications

References 25 publications

Potensi bakteri lumpur minyak sebagai penghasil biosurfaktan dan antimikroba

Potensi bakteri lumpur minyak sebagai penghasil biosurfaktan dan antimikroba

Optimization and Evaluation of Biosurfactant Produced by Pantoea sp. Using Pineapple Peel Residue, Vegetable Fat and Corn Steep Liquor

Semicontinuous sophorolipid fermentation using a novel bioreactor with dual ventilation pipes and dual sieve‐plates coupled with a novel separation system

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