2002
DOI: 10.1007/s11746-002-0572-9
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Production of structured TAG rich in 1,3‐dicapryloyl‐2‐γ‐linolenoyl glycerol from borage oil

Abstract: γ-Linolenic acid (GLA) has the physiological functions of modulating immune and inflammatory responses. We produced structured TAG rich in 1,3-dicapryloyl-2-γ-linolenoyl glycerol (CGC) from GLA-rich oil (GLA45 oil; GLA content, 45.4 wt%), which was prepared by hydrolysis of borage oil with Candida rugosa lipase having weak activity on GLA. A mixture of GLA45 oil/caprylic acid (CA) (1:2, w/w) was continuously fed into a fixed-bed bioreactor (18 × 180 mm) packed with 15 g immobilized Rhizopus oryzae lipase at 30… Show more

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Cited by 25 publications
(14 citation statements)
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“…The resulting AG-CLA was composed of 74.4 mol% TAG and 25.6 mol% DAG. When this preparation underwent acidolysis with C 8 FA using immobilized R. miehei lipase, TAG were converted efficiently to MLM-structured TAG, and DAG were converted to MMM and MLM because acyl migration, esterification of DAG with C 8 FA, and acidolysis of DAG and TAG with C 8 FA occurred simultaneously during the reaction (27,28). Hence, the AG-CLA preparation was used as a starting material without further purification.…”
Section: Resultsmentioning
confidence: 99%
“…The resulting AG-CLA was composed of 74.4 mol% TAG and 25.6 mol% DAG. When this preparation underwent acidolysis with C 8 FA using immobilized R. miehei lipase, TAG were converted efficiently to MLM-structured TAG, and DAG were converted to MMM and MLM because acyl migration, esterification of DAG with C 8 FA, and acidolysis of DAG and TAG with C 8 FA occurred simultaneously during the reaction (27,28). Hence, the AG-CLA preparation was used as a starting material without further purification.…”
Section: Resultsmentioning
confidence: 99%
“…However, BO has a protective effect against morphological changes induced by γ-irradiation, and the improvement is more pronounced in both irradiated BO post-treated and prepost-treated groups at the 15 th day. This may be due to the fatty acids composition of BO, which promises the useful protector against the production of free radicals induced by gamma irradiation (Kawashima et al, 2002).…”
Section: Discussionmentioning
confidence: 99%
“…As clarified previously, when a mixture of partial acylglycerols and TAG underwent acidolysis with CA, part of the partial acylglycerols was converted to tricaprylin and MLM-type structured TAG by simultaneous esterification and acyl migration (23). The following experiments were therefore performed using the three oils containing partial acylglycerols as substrates.…”
Section: Resultsmentioning
confidence: 99%