Sweet sorghum (Sorghum bicolor) is a promising alternative crop for bioethanol production in developing countries. However, to extend the cultivative area of this crop, it needs to develop an appropriate growing protocol for farmers. This chapter describes the examination of different doses of fertilizers combined with manure and micronutrients, in various applied times, on biomass, sugar content and ethanol production of sweet sorghum. It was observed that the application of 90 N + 90 P 2 O 5 + 60 K 2 O provided maximum stem yield and optimum contents of sugar and ethanol yield, however nontreatment of any among P, P 2 O 5 and K 2 O caused significant reduction of biomass and ethanol production. Higher fertilization >90 N may provide greater productivity of this crop but it may cause lodging and economic deficit for farmers in developing countries. It was also found that the applied times of fertilization should be at 3-4 to 7-8 leaf stage. In contrast, when the fertilization was as close to the flowering stage caused remarkable reduction of stem yield and ethanol production. The supplementation of (NH4) 2 MO 7 O 2 .4H 2 O at 5 kg/ha provided an increase of 10-12 tons/ha of stem yield and a remarkable enrichment of ethanol production. Findings of this study are useful for farmers and agricultural extensionists to promote biomass and ethanol productivity of this crop for bioethanol production. This research also highlights a greater possibility of exploiting sweet sorghum cultivation in infertile and hilly, abandoned areas for ethanol production.