Production of taxadiene from cultured ginseng roots transformed with taxadiene synthase gene

Cha, Mijeong; Shim, Sang Hee; Kim, Sung Hong; Kim, Ok-Tae; Lee, Se-Weon; Kwon, Suk‐Yoon; Baek, Kwang‐Hyun

doi:10.5483/bmbrep.2012.45.10.085

Cited by 26 publications

(15 citation statements)

References 33 publications

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“…As a result, the MEP-derived primary metabolites were not synthesized in sufficient amounts to sustain normal growth. Only a few attempts led to the production of taxadiene in plants without any defect, as in ginseng (Panax ginseng; Cha et al, 2012) and N. benthamiana (Hasan et al, 2014) by the ubiquitous overexpression of the taxadiene synthase, but the amount produced was quite low.…”

Section: Diterpene Engineeringmentioning

confidence: 99%

Plant Glandular Trichomes: Natural Cell Factories of High Biotechnological Interest

2017

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ORCID IDs: 0000-0001-9302-405X (A.H.); 0000-0002-2315-6900 (M.B.); 0000-0002-3688-7614 (C.H.).Multicellular glandular trichomes are epidermal outgrowths characterized by the presence of a head made of cells that have the ability to secrete or store large quantities of specialized metabolites. Our understanding of the transcriptional control of glandular trichome initiation and development is still in its infancy. This review points to some central questions that need to be addressed to better understand how such specialized cell structures arise from the plant protodermis. A key and unique feature of glandular trichomes is their ability to synthesize and secrete large amounts, relative to their size, of a limited number of metabolites. As such, they qualify as true cell factories, making them interesting targets for metabolic engineering. In this review, recent advances regarding terpene metabolic engineering are highlighted, with a special focus on tobacco (Nicotiana tabacum). In particular, the choice of transcriptional promoters to drive transgene expression and the best ways to sink existing pools of terpene precursors are discussed. The bioavailability of existing pools of natural precursor molecules is a key parameter and is controlled by so-called cross talk between different biosynthetic pathways. As highlighted in this review, the exact nature and extent of such cross talk are only partially understood at present. In the future, awareness of, and detailed knowledge on, the biology of plant glandular trichome development and metabolism will generate new leads to tap the largely unexploited potential of glandular trichomes in plant resistance to pests and lead to the improved production of specialized metabolites with high industrial or pharmacological value.

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Section: Diterpene Engineeringmentioning

confidence: 99%

Plant Glandular Trichomes: Natural Cell Factories of High Biotechnological Interest

2017

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“…One of the major reasons for using non-model plant hosts is the presence of specic upstream substrates in the selected organism. For example, Artemisia annua and ginseng (Panax ginseng) have been utilized to synthesize taxadiene, 42,43 due to the abundance of terpene precursors in these plants. A. annua grows relatively quickly and has an efficient genetic transformation system and ginseng root cultures can be transformed to functionally express heterologous plant enzymes.…”

Section: Selecting a Heterologous Plant Host For Phytochemical Producmentioning

confidence: 99%

Synthetic biology strategies toward heterologous phytochemical production

Kotopka

Smolke

2018

Nat. Prod. Rep.

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Covering: 2006 to 2018 Phytochemicals are important sources for the discovery and development of agricultural and pharmaceutical compounds, such as pesticides and medicines. However, these compounds are typically present in low abundance in nature, and the biosynthetic pathways for most phytochemicals are not fully elucidated. Heterologous production of phytochemicals in plant, bacterial, and yeast hosts has been pursued as a potential approach to address sourcing issues associated with many valuable phytochemicals, and more recently has been utilized as a tool to aid in the elucidation of plant biosynthetic pathways. Due to the structural complexity of certain phytochemicals and the associated biosynthetic pathways, reconstitution of plant pathways in heterologous hosts can encounter numerous challenges. Synthetic biology approaches have been developed to address these challenges in areas such as precise control over heterologous gene expression, improving functional expression of heterologous enzymes, and modifying central metabolism to increase the supply of precursor compounds into the pathway. These strategies have been applied to advance plant pathway reconstitution and phytochemical production in a wide variety of heterologous hosts. Here, we review synthetic biology strategies that have been recently applied to advance complex phytochemical production in heterologous hosts.

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“…The most renowned diterpenoid is the highly functionalized paclitaxel that is used as an efficient drug for the treatment of several types of cancer (Cusido et al ., ). As paclitaxel is found only in minute amounts in the bark of the Pacific yew tree ( Taxus brevifolia ), much effort has been expended in recent years to produce taxol precursors in heterologous microbial hosts such as yeast (Engels et al ., ) and E. coli (Ajikumar et al ., ) and in plants such as Arabidopsis thaliana (Besumbes et al ., ), tomato (Kovacs et al ., ), A. annua (Li et al ., ), ginseng (Cha et al ., ), tobacco (Rontein et al ., ; Hasan et al ., ) and the moss Physcomitrella patens (Anterola et al ., ).…”

Section: Engineering Of (Novel) Diterpenoids In Photosynthetic Organismsmentioning

confidence: 99%

Synthetic biology for production of natural and new‐to‐nature terpenoids in photosynthetic organisms

et al. 2016

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SUMMARYWith tens of thousands of characterized members, terpenoids constitute the largest class of natural compounds that are synthesized by all living organisms. Several terpenoids play primary roles in the maintenance of cell membrane fluidity, as pigments or as phytohormones, but most of them function as specialized metabolites that are involved in plant resistance to herbivores or plant-environment interactions. Terpenoids are an essential component of human nutrition, and many are economically important pharmaceuticals, aromatics and potential next-generation biofuels. Because of the often low abundance in their natural source, as well as the demand for novel terpenoid structures with new or improved bioactivities, terpenoid biosynthesis has become a prime target for metabolic engineering and synthetic biology projects. In this review we focus on the creation of new-to-nature or tailor-made plant-derived terpenoids in photosynthetic organisms, in particular by means of combinatorial biosynthesis and the activation of silent metabolism. We reflect on the characteristics of different potential photosynthetic host organisms and recent advances in synthetic biology and discuss their utility for the (heterologous) production of (novel) terpenoids.

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Production of taxadiene from cultured ginseng roots transformed with taxadiene synthase gene

Cited by 26 publications

References 33 publications

Plant Glandular Trichomes: Natural Cell Factories of High Biotechnological Interest

Plant Glandular Trichomes: Natural Cell Factories of High Biotechnological Interest

Synthetic biology strategies toward heterologous phytochemical production

Synthetic biology for production of natural and new‐to‐nature terpenoids in photosynthetic organisms

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