Production of technical-grade sodium citrate from glycerol-containing biodiesel waste by Yarrowia lipolytica

Kamzolova, Svetlana V.; Vinokurova, N. G.; Lunina, Julia N.; Zelenkova, N. F.; Morgunov, Igor G.

doi:10.1016/j.biortech.2015.06.092

Cited by 30 publications

(22 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Citric acid production increased during the later stages of cultivation which coincided with the depletion of lipids. Similar to previous reports, the re-consumption of citric acid during the course of cultivation in shake flasks was not observed [26, 27]. On the other hand, the total biomass concentration slightly increased at the later fermentation stages, suggesting the synthesis of cellular compounds other than lipids (i.e.…”

Section: Resultssupporting

confidence: 89%

Substrates and oxygen dependent citric acid production by Yarrowia lipolytica: insights through transcriptome and fluxome analyses

et al. 2017

View full text Add to dashboard Cite

BackgroundUnlike the well-studied backer yeast where catabolite repression represents a burden for mixed substrate fermentation, Yarrowia lipolytica, an oleaginous yeast, is recognized for its potential to produce single cell oils and citric acid from different feedstocks. These versatilities of Y. lipolytica with regards to substrate utilization make it an attractive host for biorefinery application. However, to develop a commercial process for the production of citric acid by Y. lipolytica, it is necessary to better understand the primary metabolism and its regulation, especially for growth on mixed substrate.ResultsControlling the dissolved oxygen concentration (pO2) in Y. lipolytica cultures enhanced citric acid production significantly in cultures grown on glucose in mono- or dual substrate fermentations, whereas with glycerol as mono-substrate no significant effect of pO2 was found on citrate production. Growth on mixed substrate with glucose and glycerol revealed a relative preference of glycerol utilization by Y. lipolytica. Under optimized conditions with pO2 control, the citric acid titer on glucose in mono- or in dual substrate cultures was 55 and 50 g/L (with productivity of 0.6 g/L*h in both cultures), respectively, compared to a maximum of 18 g/L (0.2 g/L*h) with glycerol in monosubstrate culture. Additionally, in dual substrate fermentation, glycerol limitation was found to trigger citrate consumption despite the presence of enough glucose in pO2-limited culture. The metabolic behavior of this yeast on different substrates was investigated at transcriptomic and 13C-based fluxomics levels.ConclusionUpregulation of most of the genes of the pentose phosphate pathway was found in cultures with highest citrate production with glucose in mono- or in dual substrate fermentation with pO2 control. The activation of the glyoxylate cycle in the oxygen limited cultures and the imbalance caused by glycerol limitation might be the reason for the re-consumption of citrate in dual substrate fermentations. This study provides interesting targets for metabolic engineering of this industrial yeast.Electronic supplementary materialThe online version of this article (doi:10.1186/s12934-017-0690-0) contains supplementary material, which is available to authorized users.

show abstract

Section: Resultssupporting

confidence: 89%

Substrates and oxygen dependent citric acid production by Yarrowia lipolytica: insights through transcriptome and fluxome analyses

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Citric acid production values achieved (results of the strain ACA YC 5029 in the bioreactor trial where CA max = 39 g/L and Y CA/Glol = 0.42 g/g) can be considered as relatively satisfactory, but lower as compared with the highest values reported so far in the literature; for instance, CA max quantities within the range of 63–157 g/L have been reported by wild or mutant Y. lipolytica strains cultivated on ethanol , vegetable oils , (industrial) glycerol , or a so‐called “glycerol waste” (a waste of biodiesel‐production facilities composed of both glycerol and free fatty acids employed as substrates) in bioreactor experiments with various configurations employed. Likewise, the maximum values of Y CA/Glol in the international literature reported were 0.90–0.95 g/g , which are clearly higher than the one obtained in the current investigation.…”

Section: Discussionmentioning

confidence: 93%

Production of secondary metabolites through glycerol fermentation under carbon‐excess conditions by the yeasts Yarrowia lipolytica and Rhodosporidium toruloides

Papanikolaou

Kampisopoulou

Blanchard

et al. 2017

Euro J Lipid Sci & Tech

View full text Add to dashboard Cite

The biochemical behavior of the yeasts Rhodosporidium toruloides DSM 4444, Yarrowia lipolytica ACA YC 5029, and Y. lipolytica ACA YC 5030 was evaluated on waste glycerol‐based media, added at increasing initial concentrations (50–140 g/L), with nitrogen remaining constant. During flask cultivation of Y. lipolytica ACA‐YC 5030, and although medium pH remained >4.8, major metabolites were the polyols mannitol (CMl) and erythritol (CEr) (CMlmax = 32.1 g/L, conversion yield per glycerol consumed =0.23 g/g, and CErmax = 35.5 g/L, yield ≈0.25 g/g). For the strain ACA‐YC 5029, equally in flasks and pH >4.8, the major metabolites were again mannitol and erythritol, with CMlmax = 28.9 g/L (conversion yield ≈0.21 g/g), and CErmax = 33.6 g/L (yield ≈0.24 g/g). In contrast, in batch‐bioreactor experiment, the yeast ACA YC‐5029 produced mainly citric acid (maximum concentration =39 g/L, yield on glycerol =0.42 g/g). R. toruloides DSM 4444 was flask‐cultured at increasing initial glycerol quantities (up to ≈140 g/L) and displayed significant growth producing also satisfactory amounts of lipids. The maximum DCW concentration achieved was 39.1 g/L. Maximum lipids were 13.7 g/L (at that point lipid in DCW was 37.0% w/w). The major cellular fatty acid produced was oleic acid. Phospholipids of R. toruloides were the most unsaturated fraction among lipid fractions quantified. Practical applications: Industrial glycerol, the main by‐product of biodiesel‐producing facilities, appears in constantly increasing quantities in the market volume due to the worldwide rise of biodiesel production, rendering the valorization of this by‐product as a very important scientific priority. In this report, the yeasts Y. lipolytica ACA YC 5029 and Y. lipolytica ACA YC 5030 were cultivated in submerged batch experiments with industrial glycerol as substrate under nitrogen limitation and appreciable quantities of mannitol and erythritol, compounds presenting significant importance for the food industries, were synthesized. The yeast R. toruloides DSM 4444, equally batch‐cultured on glycerol under nitrogen limitation, produced appreciable quantities of biomass that contained high concentrations of microbial lipids, that could be transformed into “2nd generation” biodiesel. The current study, hence, provides viable possibilities of glycerol valorization through its use as substrate in order metabolic compounds of added‐value to be synthesized. Crude glycerol, the principal “waste” stream deriving from biodiesel production process, is converted with the aid of microbial fermentations into secondary metabolites useful for the food‐ and biofuels‐producing industries. The metabolic compounds synthesized are microbial lipids (single cell oils) amenable to be converted into 2nd generation biodiesel with fermentations led by a strain of the yeast Rhodosporidium toruloides and mannitol, erythritol and citric acid with fermentations led by strains of the yeast Yarrowia lipolytica.

show abstract

“…Y. lipolytica was classified as a GRAS (generally recognizes as safe) microorganism and therefore can easily be used in industry (Groenewald et al, 2014). It was shown that Y. lipolytica can convert pure or crude glycerol into organic acids, polyols, single cell oils (SCO), and single cell proteins (SCP) in different cultivation systems (Dobrowolski et al, 2013;Kamzolova et al, 2015;Lazar et al, 2014;Miroń czuk et al, 2014;Rywiń ska et al, 2013;Rymowicz et al, 2010).…”

Section: Introductionmentioning

confidence: 98%

A two-stage fermentation process of erythritol production by yeast Y. lipolytica from molasses and glycerol

Mirończuk

Rakicka

Biegalska

et al. 2015

Bioresource Technology

View full text Add to dashboard Cite

Production of technical-grade sodium citrate from glycerol-containing biodiesel waste by Yarrowia lipolytica

Cited by 30 publications

References 28 publications

Substrates and oxygen dependent citric acid production by Yarrowia lipolytica: insights through transcriptome and fluxome analyses

Substrates and oxygen dependent citric acid production by Yarrowia lipolytica: insights through transcriptome and fluxome analyses

Production of secondary metabolites through glycerol fermentation under carbon‐excess conditions by the yeasts Yarrowia lipolytica and Rhodosporidium toruloides

A two-stage fermentation process of erythritol production by yeast Y. lipolytica from molasses and glycerol

Contact Info

Product

Resources

About