Production of Tetracycline by Streptomyces aureofaciens in Synthetic Media

Darken, Marjorie A.; Berenson, Herman; Shirk, Richard J.; Sjolander, Newell O.

doi:10.1128/aem.8.1.46-51.1960

Cited by 36 publications

(24 citation statements)

References 10 publications

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“…The TC concentration value is similar to values usually found in fermentation broth media (0.2-2 g L 21 ). 39,45 The biphasic mixture was mechanically stirred (vortex) and left to equilibrate for 12 h to achieve a complete partitioning of the molecule between the two phases. The partitioning solute quantification, in both phases, was accomplished by UV spectroscopy using a SHIMADZU UV-1700, Pharma-Spec spectrometer, at the wavelength of 276 nm, using calibration curves previously established.…”

Section: Methodsmentioning

confidence: 99%

“…Sodium carbonate was chosen due its effectiveness in promoting ABS and based on its domestically well known use and applications as food additive; besides, carbonate is a common species already present in the broth, as calcium carbonate is usually added to the fermentative media as it proved to enhance TC production. 39 To study the influence of the cation structure of the IL in the formation of ABS, the ternary phase diagrams were determined at 25 C for 13 chloride-based ILs. From the gathered phase diagrams, the most representative systems were selected and screened through their efficiencies in the partitioning of TC and TC.HCl.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Aqueous biphasic systems composed of ionic liquids and sodium carbonate as enhanced routes for the extraction of tetracycline

Marques

Mourão

Neves

et al. 2013

Biotechnology Progress

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Aqueous biphasic systems (ABS) using ionic liquids (ILs) offer an alternative approach for the extraction, recovery, and purification of biomolecules through their partitioning between two aqueous liquid phases. In this work, the ability of a wide range of ILs to form ABS with aqueous solutions of Na2 CO3 was evaluated. The ABS formed by IL + water + Na2 CO3 were determined at 25°C, and the respective solubility curves, tie-lines, and tie-line lengths are reported. The studied ILs share the common chloride anion, allowing the IL cation core, the cation isomerism, the presence of functionalized groups, and alkyl side chain length effects to be evaluated. An increase in the cation side alkyl chain length leads to a higher ability for liquid-liquid demixing whereas different positional isomers and the presence of an allyl group have no major influence in the phase diagrams behavior. Quaternary phosphonium- and ammonium-based fluids are more able to form an ABS when compared with imidazolium-, pyridinium-, pyrrolidinium-, and piperidium-based ILs. Moreover, the presence of an aromatic cation core has no major contribution to the formation of ABS when compared to the respective nonaromatic counterparts. Finally, to appraise on the systems applicability in downstream processing, selected systems were used for the partitioning of tetracyclines (neutral and salt forms) - a class of antibiotics produced by bacteria fermentation. Single-step extraction efficiencies for the IL-rich phase were always higher than 99% and confirm the great potential of ILs to be applied in the biotechnological field.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Aqueous biphasic systems composed of ionic liquids and sodium carbonate as enhanced routes for the extraction of tetracycline

Marques

Mourão

Neves

et al. 2013

Biotechnology Progress

View full text Add to dashboard Cite

show abstract

“…[52] Tetracyclines are another class of antibiotics produced by the same members of soil-dwelling bacteria, Streptomyces aureofaciens. [53] Similarly, Aminoglycosides are a group of antibiotics released by Streptomyces and Micromonospora. [54] Their mode of action involves targeting of protein synthesis, and they are effective against aerobic, Gram-negative bacteria and M. tuberculosis.…”

Section: Bacterial Sourcesmentioning

confidence: 99%

Antimicrobial discovery from natural and unusual sources

Ali

Siddiqui

Khan

2018

Journal of Pharmacy and Pharmacology

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Animals living in polluted environments are an underutilized source for potential antimicrobials, hence it is believed that several novel bioactive molecule(s) will be identified from these sources to counter increasingly resistant bacterial infections. Further research will be necessary in the development of novel antimicrobial(s) from these unusual sources which will have huge clinical impact worldwide.

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“…Many bacteria are considered to be harmful pathogens in several plants and animals including human beings (Duc et al, 2005;DuPont, 2007;Apetroaie-Constantin et al, 2009). However, certain bacterial strains are used as materials for genetic engineering and an industrial workhorse for the economic production of numerous enzymes, proteins, and antibiotics (Darken et al, 1960;Konings et al, 1997;Clardy et al, 2009). B. subtilis as a model of Gram-positive bacteria have been intensively characterized in a variety of ways for the microbiological research as well as biotechnology (Härtig and Jahn, 2012;Thwaite and Atkins, 2012).…”

Section: Introductionmentioning

confidence: 99%

Immobilized Polydiacetylene Lipid Vesicles on Polydimethylsiloxane Micropillars as a Surfactin-Based Label-Free Bacterial Sensor Platform

et al. 2018

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Accurate detection and sensing of bacteria are becoming increasingly important not only in microbiology but in a variety of fields including medicine, food, public health, and environmental science. However, even new rapid methods are not convenient enough.Here, we describe a simple and efficient label-free bacterial detection system using the polydiacetylene (PDA) liposomes immobilized on the 3D polydimethylsiloxane (PDMS) micropillars. Our system utilizes the colorimetric response of amine functionalized PDA vesicles to surfactin, a bacterial cyclic lipopeptide commonly released by Gram-positive Bacillus species as an antibiotic. To improve the sensitivity of PDA vesicles to surfactin by increasing the number and surface area of immobilized vesicles, the PDA vesicles were immobilized on the micropillar structure to give a hierarchical 3D PDA vesicle structure. For the fabrication of the 3D micropillar structure, polydimethylsiloxane (PDMS) was used to overcome the limitations imposed by silicon-based fabrication. In contrast to the 2D-PDA-PDMS system, which could only hardly detect the presence of 500 µM surfactin, the 3D-PDA-PDMS system could efficiently detect the presence of 5 µM surfactin and the initial presence of 4 × 10 1 cells/ml of Bacillus subtilis NCIB3610, which actively produces surfactin. Furthermore, bacterial strains that are known to produce no surfactin, such as Staphylococcus aureus Newman, Escherichia coli DH5α, and Pseudomonas aeruginosa PA14 were not detected by our system suggesting that the 3D-PDA-PDMS system is highly specific to surfactin but not to other chemicals produced by bacteria. Taken together, our results suggest that the 3D-PDA-PDMS system can sensitively and selectively be used for the high throughput detection and screening of biotechnologically important surfactin-producing bacterial strains.

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Production of Tetracycline by Streptomyces aureofaciens in Synthetic Media

Abstract: Importance of the transfer of oxygen for the production of antibiotics. I. Application of the sulfite method for fermentation on shaker apparatuses. Ceskoslov. mikrobiol., 2, 37-42.

Cited by 36 publications

References 10 publications

Aqueous biphasic systems composed of ionic liquids and sodium carbonate as enhanced routes for the extraction of tetracycline

Aqueous biphasic systems composed of ionic liquids and sodium carbonate as enhanced routes for the extraction of tetracycline

Antimicrobial discovery from natural and unusual sources

Immobilized Polydiacetylene Lipid Vesicles on Polydimethylsiloxane Micropillars as a Surfactin-Based Label-Free Bacterial Sensor Platform

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