Production of Tetramethylpyrazine from Cane Molasses by Bacillus sp. TTMP20

Abstract

2,3,5,6-Tetramethylpyrazine (TTMP) is an active ingredient of Ligusticum wallichii Franch. It can be used in medicine and food fields. In this study, Bacillus sp. TTMP20 was applied to produce TTMP using cane molasses as a carbon source. After pretreatment with phosphoric acid, 170 mL/L treated molasses, combined with 10 g/L yeast powder, 30 g/L tryptone and 30 g/L (NH4)2HPO4 were used for fermentation. After 36 h, TTMP output reached the highest value of 208.8 mg/L. The yield of TTMP using phosphoric acid-treated molasses as carbon source was 145.59% higher than control. Under the sulfuric acid treatment process of molasses (150 g), the maximum yield of TTMP was 895.13 mg/L, which was 183.18% higher than that of untreated molasses (316.1 mg/L). This study demonstrated that molasses is a high-quality and inexpensive carbon source for the manufacture of TTMP, laying the groundwork for the future industrial production of TTMP.

Optimization of Molasses and Soybean Meal Content to Enhance Tetramethylpyrazine Yield by Bacillus sp. TTMP20

“…In our previous study published in Molecules , the production of TTMP using molasses instead of glucose achieved good results, but the maximum TTMP yield did not exceed 1000 mg/L [ 27 ]. In this study, the use of molasses instead of glucose as the carbon source and soybean meal instead of tryptone as the nitrogen source resulted in a maximum yield of TTMP that reached 1328.95 mg/L.…”

“…In our previous study, the ability of a cheap carbon source (molasses) to produce TTMP was investigated [ 27 ]. The highest TTMP yield (895.13 mg/L) was obtained after 36 h of fermentation with molasses as the carbon source and tryptone as the organic nitrogen source.…”

High astaxanthin production by Xanthophyllomyces dendrorhous strain DW6 from cane molasses using two-stage pH strategies

“…42 Our studies have shown that strain DW6 had a broad spectrum of carbons including monosaccharide and disaccharide, and nitrogen sources including organic and inorganic ones, indicating that the waste sugarcane molasses can be used as both carbon and nitrogen substrates. 43 Therefore, batch fermentation using sugarcane molasses as substrates without the supplementation of nitrogen sources by strain DW6 was first carried out in shaking flasks. As shown in Fig.…”