Production of tetraploid plants of Trollius chinensis Bunge induced by colchicine

Zhang, Q.; Zhang, F.; Li, B.; Zhang, L.; Shi, Huanzhi

doi:10.17221/89/2015-cjgpb

Cited by 16 publications

(7 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This result revealed that inflorescences diameter of Calendula plants increased as concentration rate of colchicine increased and reached its peak values at 0.05 per cent. Similar results were reported by various authors as (Zhang et al, 2016) on Trollius chinensis (Wang et al, 2017) on Fagopyrum tataricum and (El-Nashar and Ammar 2016) on C. officinalis. They found that colchicine treatments had a positive and significant effect on diameter of inflorescences compared to untreated plants.…”

Section: Diameter Of Inflorescencesupporting

confidence: 91%

Morphological and Genetic Diversity Analysis in Calendula (Calendula Officinalis L.) Influenced by Mutagenic Effect of Colchicine

Yassein¹

2021

J microb biotech food sci

View full text Add to dashboard Cite

Calendula officinalis L. (pot marigold) is one of the main aromatic and medicinal plants with many uses in food and medicines. This study was carried out to demonstrate the efficiency of six colchicine concentrations (0.025, 0.05, 0.1, 0.2, 0.4 and 0.8 per cent, w/v) for Calendula improvement and induction of genetic variation. Colchicine treatments had a positive effect on the number of branches/plant, number of inflorescences, fresh and dry weight of inflorescences, inflorescence diameter, total soluble carbohydrates and β-carotene except for plant height, while seed germination and plant height were reduced. Estimation of heritability, genetic advance, genetic variability and selection of superior genotypes will be an important object in crop breeding and genetic improvement programs, and selection of genotypes with higher desirable characters. Heritability was high and ranged from 48.64 to 90.81, respectively (inflorescence diameter and plant height, respectively). Molecular markers based on a RAPD-PCR study elucidate the classification of induced Calendula mutants into two clusters. The coefficient of genetic diversity was estimated at 30%. A combination of morphological and physiological responses with molecular data contained in the various colchicine treatments illustrated the utility of RAPD-PCR as a method for identifying useful mutants and could be used to detect the colchicine effect significantly. Findings recommend the 0.05 per cent colchicine for efficient breeding calendula mutation and genetic improvement.

show abstract

Section: Diameter Of Inflorescencesupporting

confidence: 91%

Morphological and Genetic Diversity Analysis in Calendula (Calendula Officinalis L.) Influenced by Mutagenic Effect of Colchicine

Yassein¹

2021

J microb biotech food sci

View full text Add to dashboard Cite

show abstract

“…It also informed that the stomata size of the tetraploids larger but less than diploid plants [ 4 , 21 ]. In comparison with diploid plants, the tetraploids showed an increase not only in the stomatal size but also the chloroplast numbers in guard cell [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

“…On the contrary, colchicine reduced the plant height of Impatiens balsamina at three weeks after planting while plant stem diameter increased [ 35 ]. The tetraploid plants achieved by colchicine treatment showed an increase in phenotypic features such as leaf length and width, petiole, flower, and pollen diameters, but decreased in the pollen fertility [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Colchicine effects on the ploidy level and morphological characters of Katokkon pepper (Capsicum annuum L.) from North Toraja, Indonesia

Tammu

Nuringtyas

Daryono

2021

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

Background Productivity and quality of crops can be increased through polyploidy plants induced by colchicine. The use of colchicine has never been applied to Katokkon pepper, a local red pepper variety from North Toraja Indonesia. This pepper is characterized by its unique shape like the small-bell pod type of paprika and its strong spicy taste. Therefore, this study aimed to determine the effects of colchicine treatment on the ploidy level and morphological characters of Katokkon pepper. Results Flow cytometer analysis showed that all colchicine concentration treatments ranging from 0.025 to 0.1% with 24 h immersion time generated two plant groups based on their ploidy level; 50% of the total treatment plants was diploid while the rest plants were mixoploid. All colchicine treatment plants were significantly different in their width of stomata guard cells from the control plant. The effect of colchicine was also significantly on the plant height, thickness of fruit flesh, and the number of fruits per plant. Conclusions The results of this study showed that colchicine treatment had a significant effect on the ploidy level and several morphological characters of the Katokkon pepper. The colchicine treatment increased the number of fruits per plant and thicker flesh of fruits but reduced the size and weight of Katokkon pepper. Our findings provide essential information to obtain tetraploid Katokkon plants through colchicine treatment in further research. This study benefits as a preliminary step for increasing the productivity and quality of the local red peppers in Indonesia.

show abstract

“…Addition of 0.01 % of colchicine could induced polyploidy in Bacopa monnieri [26] and mixoploidy in Physalis peruviana L. [27]. Furthermore, addition of 0.1 % colchicine in Trollius chinensisis thought to be optimal to induce the polyploid plants [28].…”

Section: Ploidy Level Identificationmentioning

confidence: 98%

Effect of Colchicine on In Vitro Growth and Ploidicity of Crown Vetiver Plant (Vetiveria zizanioides L. Nash)

Sinta

Widoretno

2020

JELS

View full text Add to dashboard Cite

The goal of this research was to evaluate the effect of colchicine on the formation and growth of shoot from explant crowns by in vitro, as well as to obtain polyploidy vetiver plant (Vetiveriazizanioides L. Nash). Induction of polyploidy vetiver plants carried out by culturing explant crowns on MS media supplemented by 2 mg.L-1 BAP and colchicine (0, 30, 60, 90, 120 mg.L-1) for three weeks. Explant and formed shoot regenerated on MS media containing 1 mg.L-1 NAA. The generated plantlets acclimatized on the growing media (cocopeat: husk charcoal: compost = 1: 1: 2). Plant ploidy level of the plants regenerated from treated explant estimated by chromosome counting in root tips. The effect of colchicine on media was able to induce polyploidy in vetiver plants. Five mixoploids were obtained from explant treated colchicine. The vetiver mixoploid plants obtained were 20% and 62.5% from colchicine 60 mg.L-1 and 90 mg.L-1, respectively. The vetiver mixoploid plants consist of diploid (2n=2x=20) and triploid cells (2n=3x=30) or diploid (2n=2x=20) and tetraploid (2n=4x=40). The addition of colchicine in concentrations above 30 mg.L-1 caused inhibition of shoot formation and growth, even a concentration of 120 mg.L-1 caused explant death. However, 30 mg.L-1 colchicine could increase the number of formed shoots, while only 60 mg.L-1 and 90 mg.L-1 could be induced the mixoploidy in the vetiver plant. The results showed that colchicine treatment could increase ploidicity in vetiver plants in vitro, but caused inhibition of shoot formation and growth.

show abstract

Production of tetraploid plants of Trollius chinensis Bunge induced by colchicine

Cited by 16 publications

References 15 publications

Morphological and Genetic Diversity Analysis in Calendula (Calendula Officinalis L.) Influenced by Mutagenic Effect of Colchicine

Morphological and Genetic Diversity Analysis in Calendula (Calendula Officinalis L.) Influenced by Mutagenic Effect of Colchicine

Colchicine effects on the ploidy level and morphological characters of Katokkon pepper (Capsicum annuum L.) from North Toraja, Indonesia

Effect of Colchicine on In Vitro Growth and Ploidicity of Crown Vetiver Plant (Vetiveria zizanioides L. Nash)

Contact Info

Product

Resources

About