Production of the BmCecB1 antimicrobial peptide in transgenic silkworm

Abstract

This peptide has antibacterial activity against several Gram-positive and Gram-negative bacteria. Bombyx mori cecropinB1(BmCecB1) is antimicrobial peptides from Bombyx mori and belongs to cecropin family. Antimicrobial peptides are important components of the innate immune systems in all living organism. To produce the BmCecB1 antimicrobial peptide, we constructed transgenic silkworm that expressed BmCecB1 gene under the control BmA3 promoter using piggyBac vector. The use of the 3xP3-driven EGFP cDNA as a marker allowed us to rapidly distinguish transgenic silkworm. Mixtures of the donor vector and helper vector were micro-injected into 600 eggs of bivoltin silkworms, Baegokjam. In total, 49 larvae (G0) were hatched and allowed to develop into moths. The resulting G1 generation consisted of 22 broods, and we selected 2 broods containing at least 1 EGFP-positive embryo. The rate of successful transgenesis for the G1 broods was 9%. We identified 9 EGFP-positive G1 moths and these were backcrossed with wild-type moths. With the aim of identifying a BmCecB1 as antimicrobial peptide, we investigated the Radical diffusion Assay (RDA) and then demonstrated that BmCecB1 possesses high antibacterial activities against Gramnegative bacteria. Recently, a germline transformation method using the GCTAGCATGCGTGTTTTAATTTTCACG-3' and reverse primer 5'-CTTGGTTCGGCTAAAGCTATACTTAAG-3') into

Pap12-6: A host defense peptide with potent immunomodulatory activity in a chicken hepatic cell culture

“…Regarding the levels of EC H 2 O 2 , the two highest concentrations of PAP were able to restore the elevation caused by Poly I:C, suggesting the antioxidant nature of the HDP. Even though the impact of papiliocins on the oxidative state has not been frequently examined, our findings are in agreement with the observations on the parent peptide papiliocin, which was able to decrease the intracellular PLOS ONE H 2 O 2 levels caused by oxidative stress in CaCo-2 human colorectal adenocarcinoma cell line [71].…”

Expression of the cyan fluorescent protein in fibroin H-chain of transgenic silkworm

“…Recently, Silk-based materials with improved antibacterial properties, mechanical properties, biodegradation and biocompatibility, have been used for applications in bioengineering of bone, skin, cartilage, ligaments, tendons, vascular, neural, ocular, cardiac, and bladder tissues etc (Tatemastu et al, 2012;Zhao et al, 2009). Kim et al (2015) reported on the expression of BmcecB1 antimicrobial peptide in silkworm.…”