Production of transforming growth factor-α in human tumour cell lines

Imanishi, K; Yamaguchi, Ken; Suzuki, Masafumi; Honda, Satoshi; Yanaihara, Noboru; Abe, Kaoru

doi:10.1038/bjc.1989.159

Cited by 55 publications

(24 citation statements)

References 19 publications

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“…GCIY, GT3TKB and HGC27 cells were obtained from the RIKEN cell bank and were described previously. 12,13) The AZ521 14) cell line was obtained from the Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer, Tohoku University. The 4-1st cell line is derived from an adenocarci-C 5 To whom correspondence should be addressed.…”

Section: Methodsmentioning

confidence: 99%

Identification of candidate predictive markers of anticancer drug sensitivity using a panel of human cancer cell lines

et al. 2003

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We previously investigated the correlations between the expression of 9216 genes and various chemosensitivities in a panel of 39 human cancer cell lines 1) and found that the expression levels of AKR1B1 and CTSH were correlated with sensitivity and resistance to multiple drugs, respectively. To validate these correlations, we investigated the expression of these two genes and the chemosensitivities in 12 additional gastric cancer cell lines. The expression of AKR1B1 in the additional cell lines exhibited significant correlations with sensitivities to 8 of the 23 drugs examined, while that of CTSH displayed a significant negative correlation with only one (MS-247) of the 27 drugs examined. Their expressions were weakly correlated with sensitivity and resistance, respectively, to the remainder of the drugs. Moreover, when the 12 cell lines were divided into high-expressing and low-expressing groups, a comparison of these groups using Mann-Whitney's U test revealed that high expression levels of AKR1B1 and CTSH were related to sensitivity to 21 of the drugs and resistance to 8 of the drugs, respectively. The present results suggest that AKR1B1 and CTSH may be good markers for prediction of sensitivity to certain drugs and that our panel of 39 cell lines has the potential to identify candidate predictive marker genes. (Cancer Sci 2003; 94: 1074-1082)

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Section: Methodsmentioning

confidence: 99%

Identification of candidate predictive markers of anticancer drug sensitivity using a panel of human cancer cell lines

et al. 2003

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“…Neoplastic cells frequently produce bioactive substances, such as hormones, cytokines and growth factors (Imanishi et al, 1989;Mori et al, 1991;Odell & Appleton, 1992). These products have the potential to induce responses in remote target organs, normal tissues surrounding the cancer cells or the neoplastic cells themselves; these mechanisms of action are designated as endocrine, paracrine and autocrine respectively.…”

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confidence: 99%

Production of endothelin-1 and thrombomodulin by human pancreatic cancer cells

Oikawa¹,

Kushuhara²,

Ishikawa³

et al. 1994

Br J Cancer

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Summary Analysis of bioactive substances produced by cancer cells is one approach to understanding the biological features of human cancer. One of these bioactive substances is endothelin (ET)-1, a peptide with potent vasoconstrictive activity produced by vascular endothelial cells. We have previously reported the production of ET-1 by several types of human cancer, especially pancreatic cancer cells. To elucidate whether these cancer cells might share biological characteristics with vascular endothelial cells, we investigated the production of three ET isoforms in pancreatic cancer cells, using a specific radioimmunoassay. Further, we also investigated whether these cells produce thrombomodulin (TM), another product of endothelial cells functioning as a modulator of procoagulant activity. ET-1 was detected in 11 of 12 pancreatic cancer cell lines (92%) while ET-2 and ET-3 were detectable in only one cell line. Gel filtration analysis confirmed the presence of ET-1. Moreover, TM was detected in the cell lysates of 11 of the 12 cell lines (92%) and it was released into the culture medium in the majority (58%) of these cell lines. TM mRNA was also detected in these cells. In addition, TM was demonstrated immunocytochemically along the cell surface. These results suggest that pancreatic cancer cells share two characteristics with endothelial cells: the production of ET-1 and TM.

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“…Additionally, a tumour xenograft to nude mice of squamous cell lung carcinoma (Lu-61) was also examined. This tumour was established from a patient presenting with HHM (Kameya et al, 1982). In vitro culture of this tumour xenograft was not successful.…”

Section: Methodsmentioning

confidence: 99%

Production of parathyroid hormone-related protein in tumour xenografts in nude mice presenting with hypercalcaemia

Miyake¹,

Yamaguchi²,

Honda³

et al. 1991

Br J Cancer

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Summary This study examined the pathophysiological role of parathyroid hormone-related protein (PTHrP) in humoral hypercalcaemia of malignancy (HHM). Seven human tumour xenografts were analysed in nude mice; five tumours (KEsC-2, oesophageal carcinoma; FA-6, pancreatic carcinoma; SEKI, melanoma; Lu-65A and Lu-61, lung carcinomas) were associated with hypercalcaemia and two tumours (MIA PaCa-2, pancreatic carcinoma; PLC/PRF/5, hepatocellular carcinoma) with normocalcaemia. Northern blot analyses, radioimmunoassay and bioassay confirmed the synthesis of PTHrP-like peptides by all five tumours associated with hypercalcaemia, but not by the two associated with normocalcaemia. These observations indicated a very close relationship between the production of PTHrP and the development of HHM. Gel filtration studies of three tumour tissue extracts revealed at least two different molecules with both PTHrP-like immunological and biological activities. One peak eluted at a position between PTHrP (1-141) and cytochrome C and the other at a position identical to cytochrome C. These results suggest that PTHrP molecules with a molecular size equal to or greater than cytochrome C participate as causative agents of HHM. All five tumour xenografts caused hypercalcaemia when grown to a size of 1.5 g in nude mice. Under cell culture conditions, four original cell lines, KEsC-2, FA-6, SEKI and Lu-65A secreted 450.0, 45.0, 3.6 and 3.0 pmol of immunoreactive PTHrP/ 1.5 x 109 cells (approximately equivalent to 1.5 g wet weight) 24 h-' into their respective culture media. Since a subcutaneous infusion of 100 pmol 24 h-of PTHrP (1 -34) into nude mice was sufficient to induce significant hypercalcaemia, we speculate that PTHrP alone released from tumour cells could induce hypercalcaemia at least in the case of KEsC-2, and possibly in FA-6. With regard to other tumours associated with hypercalcaemia, further examination of PTHrP and other compounds with bone-resorbing activity in these transplantable tumours is required to obtain a better understanding of this morbidity.

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Production of transforming growth factor-α in human tumour cell lines

Cited by 55 publications

References 19 publications

Identification of candidate predictive markers of anticancer drug sensitivity using a panel of human cancer cell lines

Identification of candidate predictive markers of anticancer drug sensitivity using a panel of human cancer cell lines

Production of endothelin-1 and thrombomodulin by human pancreatic cancer cells

Production of parathyroid hormone-related protein in tumour xenografts in nude mice presenting with hypercalcaemia

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