Production of transgenic mice by random recombination of targeted genes in female germline stem cells

Zhang, Yong; Yang, Zhaojuan; Yang, Yuanyuan; Wang, Shuzeng; Shi, Lingjun; Xie, Wenhai; Sun, Kaixuan; Zou, Kang; Wang, Lei; Xiong, Ji; Xiang, Jie; Wu, Ji

doi:10.1093/jmcb/mjq043

Cited by 97 publications

(130 citation statements)

References 34 publications

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“…The claim of post-natal mammalian oogenesis polarised the scientific community, with many studies supporting the presence of OSCs (Bukovsky et al 2005, Johnson et al 2005a, Virant-Klun et al 2008, Niikura et al 2009, Zou et al 2009, Pacchiarotti et al 2010, Parte et al 2011, Zhang et al 2011, White et al 2012, Zhou et al 2014, whereas many others believed the data had been misinterpreted refuting the finding of OSCs (Bristol-Gould et al 2006, Liu et al 2007, Byskov et al 2011, Kerr et al 2012, Zhang et al 2012, Lei and Spradling 2013, Yuan et al 2013 and required further evidence before believing the existence of OSCs and the idea that this work could be extended to other mammals, including humans (Telfer 2004). However, it is questionable whether studies using alternate species can be used to refute Tilly's claims in mice.…”

Section: A New Eramentioning

confidence: 99%

“…A different group used the same method in 2011 to identify OSCs collected from transgenic mice. The isolated cells were subsequently cultured and used to produce offsprings that displayed the same transgenic mutation as the mice they were collected from (Zhang et al 2011). Ddx4 expression has subsequently been combined with fluorescent reporter activation in mice, stimulating fluorescence only in cells in which the Ddx4 gene promoter has been activated.…”

Section: Are Data Describing Oscs Comparable Between Species?mentioning

confidence: 99%

“…Studies have used mathematical, histological and ovary transplant methods to conclude that OSCs are actively dividing and undergoing meiosis in normal ovulating ovaries to maintain follicle numbers (Johnson et al 2004, Bukovsky et al 2005, Niikura et al 2009, Zou et al 2009, Parte et al 2011, Zhang et al 2011, White et al 2012, Zhou et al 2014. However, other studies, that have not found actively dividing germ cells, suggest a quiescent population of cells (Kerr et al 2006, Liu et al 2007, Kerr et al 2012, Zhang et al 2012, Lei & Spradling 2013, Yuan et al 2013.…”

Section: Do Quiescent Oscs Exist?mentioning

confidence: 99%

“…Histological ovary analyses and mating studies were performed to determine the outcome of the transplanted OSCs and their ability to restore fertility. Transplanted GFP-OSCs have not only gone on to develop into oocytes and form follicles, but have also been successfully fertilised to form transgenic offsprings, as assessed by expression of GFP (Zhang et al 2011, Zhou et al 2014. This method would provide the most convincing evidence yet for a population of OSCs that can develop into oocytes and produce offspring.…”

Section: Do Quiescent Oscs Exist?mentioning

confidence: 99%

“…The majority of studies use feeder cells, which are typically inactivated fibroblasts, to initially culture OSCs (Virant-Klun et al 2008, Zou et al 2009, Zhang et al 2011, Zhang et al 2012, Zhou et al 2014. No information is provided about potential interactions between the cultured cells and the feeder cells, or factors released by the feeder cells.…”

Section: What Are the Effects Of External Factors On Oscs?mentioning

confidence: 99%

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Oocyte stem cells: fact or fantasy?

Horan¹,

Williams²

2017

Reproduction

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For many decades, the dogma prevailed that female mammals had a finite pool of oocytes at birth and this was gradually exhausted during a lifetime of reproductive function. However, in 2004, a new era began in the field of female oogenesis. A study was published that appeared to detect oocyte-stem cells capable of generating new eggs within mouse ovaries. This study was highly controversial and the years since this initial finding have produced extensive research and even more extensive debate into their possibility. Unequivocal evidence testifying to the existence of oocyte-stem cells (OSCs) has yet to be produced, meanwhile the spectrum of views from both sides of the debate are wide-ranging and surprisingly passionate. Although recent studies have presented some convincing results that germ cells exist and are capable of creating new oocytes, many questions remain. Are these cells present in humans? Do they exist in physiological conditions in a dormant state? This comprehensive review first examines where and how the dogma of a finite pool was established, how this has been challenged over the years and addresses the most pertinent questions as to the current status of their existence, their role in female fertility, and perhaps most importantly, if they do exist, how can we harness these cells to improve a woman's oocyte reserve and treat conditions such as premature ovarian insufficiency (POI: also known as premature ovarian failure, POF).Reproduction (2017) 154 R23-R35

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Section: A New Eramentioning

confidence: 99%

Section: Are Data Describing Oscs Comparable Between Species?mentioning

confidence: 99%

Section: Do Quiescent Oscs Exist?mentioning

confidence: 99%

Section: Do Quiescent Oscs Exist?mentioning

confidence: 99%

Section: What Are the Effects Of External Factors On Oscs?mentioning

confidence: 99%

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Oocyte stem cells: fact or fantasy?

Horan¹,

Williams²

2017

Reproduction

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Isolation of Mammalian Oogonial Stem Cells by Antibody-Based Fluorescence-Activated Cell Sorting

Navaroli

Tilly

Woods

2016

Methods in Molecular Biology

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The ability to isolate and subsequently culture mitotically active female germ cells from adult ovaries, referred to as either oogonial stem cells (OSCs) or adult female germline stem cells (aFGSCs), has provided a robust system to study female germ cell development under multiple experimental conditions, and in many species. Flow cytometry or fluorescence-activated cell sorting (FACS) is an integral part of many isolation and characterization protocols. Here, we provide methodological details for antibody-based flow cytometric isolation of OSCs using antibodies specific for external epitopes of the proteins Ddx4 or Ifitm3, alone or in combination with the use of fluorescent reporter mice. Beginning with sample preparation, we provide point-by-point instructions to guide researchers on how to isolate OSCs using flow cytometry.

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Stem Cells in Mammalian Gonads

Ding

Wang

2016

Results and Problems in Cell Differentiation

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Stem cells have great value in clinical application because of their ability to self-renew and their potential to differentiate into many different cell types. Mammalian gonads, including testes for males and ovaries for females, are composed of germline and somatic cells. In male mammals, spermatogonial stem cells maintain spermatogenesis which occurs continuously in adult testis. Likewise, a growing body of evidence demonstrated that female germline stem cells could be found in mammalian ovaries. Meanwhile, prior studies have shown that somatic stem cells exist in both testes and ovaries. In this chapter, we focus on mammalian gonad stem cells and discuss their characteristics as well as differentiation potentials.

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Production of transgenic mice by random recombination of targeted genes in female germline stem cells

Cited by 97 publications

References 34 publications

Oocyte stem cells: fact or fantasy?

Oocyte stem cells: fact or fantasy?

Isolation of Mammalian Oogonial Stem Cells by Antibody-Based Fluorescence-Activated Cell Sorting

Stem Cells in Mammalian Gonads

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