Production of Transgenic Quails with High Frequency of Germ-Line Transmission Using VSV-G Pseudotyped Retroviral Vector

Mizuarai, Shinji; Ono, Kosuke; Yamaguchi, Kyoji; Nishijima, Ken ichi; Kamihira, Masamichi; Iijima, Shinji

doi:10.1006/bbrc.2001.5422

Cited by 111 publications

(73 citation statements)

References 36 publications

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“…Methylation stimulates the formation of heterochromatin, which blocks the transcriptional activity of the region surrounding the integrated retrovirus, and ultimately results in low or undetectable levels of transgene expression. This effect had been clearly documented in mice (20), and there is evidence suggesting this effect may also occur in birds (6). (ii) Retroviral LTRs contain internal promoters and enhancers, which may interfere with the expression of the transgene in both oncoretroviral and lentiviral based vectors.…”

Section: Discussionmentioning

confidence: 98%

“…Foreign DNA can be efficiently introduced into avian genomes by infecting the early embryo with oncoretroviral vectors (3). A number of groups have successfully produced transgenic chickens using this general method (4,5,6). However, in transgenic birds produced by using oncoretroviral vectors, transgene mRNA and protein product are present at low or undetectable levels, possibly due to developmental silencing (6).…”

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confidence: 99%

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Generation of tissue-specific transgenic birds with lentiviral vectors

Scott

Lois

2005

Proc. Natl. Acad. Sci. U.S.A.

133

109

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Birds are of great interest for a variety of research purposes, and effective methods for manipulating the avian genome would greatly accelerate progress in fields that rely on birds as model systems for biological research, such as developmental biology and behavioral neurobiology. Here, we describe a simple and effective method for producing transgenic birds. We used lentiviral vectors to produce transgenic quails that express GFP driven by the human synapsin gene I promoter. Expression of GFP was specific to neurons and consistent across multiple generations. Expression was sufficient to allow visualization of individual axons and dendrites of neurons in vivo by intrinsic GFP fluorescence. Tissuespecific transgene expression at high levels provides a powerful tool for biological research and opens new avenues for genetic manipulation in birds.avian ͉ genetics ͉ lentivirus ͉ synapsin ͉ neuron

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Section: Discussionmentioning

confidence: 98%

mentioning

confidence: 99%

Generation of tissue-specific transgenic birds with lentiviral vectors

Scott

Lois

2005

Proc. Natl. Acad. Sci. U.S.A.

133

109

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“…Arian leukosis virus was also used to generate chickens expressing human IFN-␣-2b at higher levels than had been reported for ␤-lactamase (3), but the efficiency with which transgenic birds were generated remained very low (a single G 1 cockerel from 1,597 chicks hatched), presumably a consequence of the low-titer vector used. Blastodermal injection of high-titer Moloney murine leukemia virus-derived vectors was used to generate transgenic quail with very high efficiency, but transgene silencing prevented detectable expression of the reporter GFP (19). A modified mouse stem cell virus injected into the heart of developing chick embryos generated transgenic hens that expressed an antiprion protein ScFv-Fc from a ubiquitous promoter, but analysis of G 0 , G 1 , and G 2 generations revealed Ϸ2-fold transgene suppression in successive generations (4).…”

Section: Discussionmentioning

confidence: 99%

Oviduct-specific expression of two therapeutic proteins in transgenic hens

Lillico

Sherman

McGrew

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

187

168

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Recent advances in avian transgenesis have led to the possibility of utilizing the laying hen as a production platform for the large-scale synthesis of pharmaceutical proteins. Ovalbumin constitutes more than half of the protein in the white of a laid egg, and expression of the ovalbumin gene is restricted to the tubular gland cells of the oviduct. Here we describe the use of lentiviral vectors to deliver transgene constructs comprising regulatory sequences from the ovalbumin gene designed to direct synthesis of associated therapeutic proteins to the oviduct. We report the generation of transgenic hens that synthesize functional recombinant pharmaceutical protein in a tightly regulated tissue-specific manner, without any evidence of transgene silencing after germ-line transmission.chicken ͉ genetic modification ͉ lentivirus ͉ pharmaceutical

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“…Replication-defective vectors allow for reasonably large exogenous genes (up to ϳ10 kb), because they are missing the viral genes necessary for replication. New retroviral vectors are being developed based upon new strategies that include pseudotyping that allows for optimal infectivity (Mizuarai et al, 2001). A common problem with some retroviral vectors used in transgenic technology has been gene silencing.…”

Section: Retroviral Overviewmentioning

confidence: 99%

“…Unfortunately, beta-galactosidase expression was only noted in cultures of embryonic fibroblasts from G2 progeny, and expression was not reported in the entire embryo. Recently, Mizuarai et al (2001) created transgenic quail by using a pseudotyped VSV-G (vesicular stomatitis virus-glycoprotein) vector, but the quail failed to adequately express the reporter gene. More recently, other groups have been successful at creating transgenic chickens using retroviral systems.…”

Section: Retroviral Historical Perspectivementioning

confidence: 99%

Status of transgenic chicken models for developmental biology

Mozdziak

Petitte

2004

Developmental Dynamics

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The chick embryo is a classic model that has been used to gain insight into developmental processes and cell fate within the embryo for over a century. For the most part, investigators have implanted quail cells into a chicken embryo. A more powerful tool for developmental biology research than the quail:chick chimera system would be to have lines of transgenic chickens expressing reporter genes that are readily available to the research community. However, avian transgenic technology has been fraught with technical difficulties, and transgenic chickens expressing reporter genes have only recently been developed. The goal of this review is to report the technologies that have been used to generate transgenic chickens and to discuss the challenges in generating avian transgenics for developmental biology research. Developmental Dynamics 229:414 -421, 2004.

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Production of Transgenic Quails with High Frequency of Germ-Line Transmission Using VSV-G Pseudotyped Retroviral Vector

Cited by 111 publications

References 36 publications

Generation of tissue-specific transgenic birds with lentiviral vectors

Generation of tissue-specific transgenic birds with lentiviral vectors

Oviduct-specific expression of two therapeutic proteins in transgenic hens

Status of transgenic chicken models for developmental biology

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