Production of triploid plants of Japanese pear (Pyrus pyrifolia Nakai) by anther culture

Kadota, Masanori; Niimi, Yoshiji

doi:10.1023/b:euph.0000046756.01934.34

Cited by 29 publications

(17 citation statements)

References 22 publications

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“…Compared with the sectioning method, samples for the squash method could be easily prepared while avoiding timeconsuming sectioning procedures (Fukui, 1996). Chromosome numbers of various deciduous fruit trees were determined using this method (Kadota and Niimi, 2004;Notsuka et al, 2000;Oginuma, 1987).…”

Section: Development Of Enzymatic Maceration Methods For Preparing Chrmentioning

confidence: 99%

Recent Progress on Studies of Chromosome Observation in Deciduous Fruit Trees

Yamamoto

2012

J. Japan. Soc. Hort. Sci.

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Information on chromosomes is essential for the progress of genetic and biotechnological studies. In this paper, recent progress on studies of chromosome observation in deciduous fruit trees is reviewed. (1) An enzymatic maceration method, preparing good chromosome samples from plants with small chromosomes, was developed for Prunus, Pyrus, Malus, and Diospyros. (2) Some morphologically similar chromosomes could be distinguished by means of the banding technique using fluorochrome staining in Prunus and Pyrus. In addition, the divergence of chromosome configuration seems to be very low or non-existent in view of fluorescent banding patterns in both genera. (3) The number and location of 5S and 18S-5.8S-25S rDNA sites were detected by fluorescent in situ hybridization (FISH) in Prunus, Pyrus, Malus, and Diospyros. The sites of retrotransposons were also visualized by FISH in Pyrus. These results provided fundamental information on the chromosomes. Chromosomal location of the S (self-incompatibility) locus in Malus was also revealed by FISH. In Diospyros, the results of FISH as well as genomic in situ hybridization (GISH) offered new findings on the phylogenetic relationships in this genus and chromosome composition of somatic hybrids.

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Section: Development Of Enzymatic Maceration Methods For Preparing Chrmentioning

confidence: 99%

Recent Progress on Studies of Chromosome Observation in Deciduous Fruit Trees

Yamamoto

2012

J. Japan. Soc. Hort. Sci.

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“…In the pear, similar studies are necessary for future development of genome analysis Yamamoto et al, 2007) and ploidy manipulation (Kadota and Niimi, 2004). Thus, there is an urgent need to perform chromosome analysis for karyological and molecular cytogenetic investigation of the pear.…”

Section: Introductionmentioning

confidence: 99%

Fluorescent Staining Analysis of Chromosomes in Pear (Pyrus spp.)

Yamamoto

Takada

Hirabayashi

et al. 2010

J. Japan. Soc. Hort. Sci.

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Fluorescent banding patterns of pear chromosomes were determined from samples taken from root tips of openpollinated seedlings of six cultivars from three species [Pyrus pyrifolia (Burm. F) Nakai (Japanese pear), P. communis L. (European pear), and P. bretschneideri Rehder (Chinese pear)]. Root tips were pretreated in 2 mM 8-hydroxyquinoline at 10°C for 4 h; the chromosome samples were prepared by the enzymatic maceration and air-drying method. All cultivars used in this study had 2n = 34 chromosomes. Chromomycin A 3 (CMA) positive (+) bands were observed in telomeric positions of four chromosomes. In some samples, these CMA + bands were observed at satellite positions. 4'-6-diamidino-2-phenylindole (DAPI) negative bands (−) were seen to correspond with CMA + bands. Thirty chromosomes had no CMA +/DAPI − bands. No propidium iodide (PI) bands were observed in any chromosomes. The number and positions of CMA + bands were stable, and there was no difference among the three species.

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“…In fruit crops, triploids have often been regenerated through anther culture in Malus £ domestica (Brokh.) (Hofer et al 2002), Pyrus pyrifolia Nakai (Kadota & Niimi 2004), and Carica papaya L. (Rimberia et al 2006).…”

Section: Characterization Of Regenerants: Ploidy Analysis and Analysimentioning

confidence: 99%

Gametic and somatic embryogenesis through in vitro anther culture of different Citrus genotypes

Cardoso

Abdelgalel

Chiancone

et al. 2015

Plant Biosystems - An International Journal Dealing with all As

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In vitro tissue culture represents a useful technique for advancing Citrus breeding and propagation. Among in vitro regeneration systems, anther culture is commonly used to produce haploids and doubled haploids for a fast-track producing homozygous lines, in comparison with the traditional self-pollination approach, which involves several generations of selfing. In addition, anthers culture can produce somatic embryos that can also be used for clonal propagation. In this study, two thermal shocks were applied to the anthers of six Citrus genotypes (two clementine and four sweet oranges), just after they were put in culture. The response obtained was different depending on the genotype: both clementines, namely Hernandina and Corsica, produced homozygous and triploid regenerants (microspore-derived embryos), whereas all of the analyzed regenerants from sweet oranges, three cultivars of Tarocco and Moro, produced heterozygous and diploid regenerants similar to the parental genotypes (somatic embryos).

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Production of triploid plants of Japanese pear (Pyrus pyrifolia Nakai) by anther culture

Cited by 29 publications

References 22 publications

Recent Progress on Studies of Chromosome Observation in Deciduous Fruit Trees

Recent Progress on Studies of Chromosome Observation in Deciduous Fruit Trees

Fluorescent Staining Analysis of Chromosomes in Pear (Pyrus spp.)

Gametic and somatic embryogenesis through in vitro anther culture of different Citrus genotypes

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