Production of Two Elite Glutinous Rice Varieties by Editing Wx Gene

Fei, Yun-Yan; Yang, Jie; Wang, Fangquan; Fan, Fangjun; Li, Wenqi; Wang, Jun; Xu, Yongsheng; Jun, Zhu; WeiGong, Zhong

doi:10.1016/j.rsci.2018.04.007

Cited by 33 publications

(12 citation statements)

References 37 publications

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“…Recently, the development of genome-editing technology, particularly the discovery and application of the CRISPR/Cas9 gene-editing system, has led to its widespread use in studying plant genome functions (Ran et al, 2013;Belhaj et al, 2015;Bortesi and Fischer, 2015). Several studies have reported that the Wx gene can be successfully edited with high efficiency in rice through the use of the CRISPR/Cas9 system, which shows a high average mutation rate of up to 80%, without affecting the major agronomic traits in the transgenic lines (Zhang et al, 2018;Yunyan et al, 2019;Huang et al, 2020). In agreement with previous studies, the wx mutation rate in the present study showed a high value of 80% and above, and wx mutants exhibited major agronomic traits similar to those of their corresponding WT lines.…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, the CRISPR/Cas9 gene-editing system has been used to knock out or fine-tune the expression of the Wx gene (Ma et al, 2015). The first exon of the Wx gene was edited to produce a null mutation via CRISPR/Cas9, to generate four japonica rice wx mutant lines with significantly reduced AC, but with an unaltered expression of agronomic traits (Zhang et al, 2018;Yunyan et al, 2019). Editing the TATA box of the Wx b promoter downregulated Wx expression and thus fine-tuned grain AC, which could improve the grain quality (Huang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Assessment of the Characteristics of Waxy Rice Mutants Generated by CRISPR/Cas9

Luo

Hua

et al. 2022

Front. Plant Sci.

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The cooking and eating quality of rice grains is a major focus from a consumer’s perspective and is mainly determined by the apparent amylose content (AAC) of the starch. Waxy rice, a type of rice with an AAC of less than 2%, is an important goal for the breeding of high-quality rice. In recent years, the cloning of the Waxy (Wx) gene has revealed the molecular mechanism of the formation of waxy traits in rice. However, there have been limited studies on the physicochemical properties, such as gelatinization temperature, rapid viscosity analyzer profile, and amylopectin fine structure of wx mutants. In the current study, a rapid and highly efficient strategy was developed through the CRISPR/Cas9 gene-editing system for generating wx mutants in the background of five different rice varieties. The wx mutation significantly reduced the AAC and starch viscosity but did not affect the major agronomic traits (such as plant height, panicle number per plant, grain number per panicle, and seed-setting frequency). Incorporation of the wx mutation into varieties with low initial AAC levels resulted in further reduction in AAC, but without significantly affecting the original, desirable gelatinization traits and amylopectin structure types, suggesting that parents with low initial AAC should be preferred in breeding programs.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Assessment of the Characteristics of Waxy Rice Mutants Generated by CRISPR/Cas9

Luo

Hua

et al. 2022

Front. Plant Sci.

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“…The waxy gene WX1 (Os06g0133000) controls the grain amylose content (AC). Mutations in this gene correlate with a phenotype that ranges from opaque (8%), semitranslucent (8-12%), and transparent (12% or more) grains (Lichun Huang et al, 2020;Sano, 1984;Xu et al, 2021;Yunyan et al, 2019;.…”

Section: Grain Number Quality Weight and Plant Structurementioning

confidence: 99%

Rice breeding in the new era: comparison of useful agronomic traits

Hernández-Soto

Echeverría-Beirute

Abdelnour-Esquivel

et al. 2021

Preprint

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Understanding agronomic traits at a genetic level enables the leveraging of this knowledge to produce crops that are more productive and resilient, have better quality and are adjusted for consumer preferences. In the last decade, rice has become a model to validate the function of specific genes, resulting in valuable but scattered information. Here, we aimed to identify particular genes in rice related to traits that can be targeted by different mutation techniques in the breeding of crops. We selected gain of function, misfunction, and specific mutations associated with phenotypes of agronomic interest. The review includes specific trait-related genes involved in domestication, stress, herbicide tolerance, pathogen resistance, grain number/quality/weight, plant structure, nitrogen use, and others. The information presented can be used for rice, other cereals, and orphan crops to achieve a superior and sustainable production in challenging farming conditions.

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“…The waxy gene WX1 (Os06g0133000) controls the grain amylose content (AC). Mutations in this gene correlate with a phenotype that ranges from opaque (8%), semitranslucent (8-12%), and transparent (12% or more) grains (Sano, 1984;Yunyan et al, 2019;Zhang et al, 2019b;Huang et al, 2020;Xu et al, 2021).…”

Section: Below)mentioning

confidence: 99%

Rice agronomic traits and variability induced by mutagenesis

Hernández-Soto¹,

Echeverría-Beirute²,

Abdelnour-Esquivel³

et al. 2021

Preprint

View full text Add to dashboard Cite

Understanding agronomic traits at a genetic level enables the leveraging of this knowledge to produce crops that are more productive and resilient, have better quality and are adjusted for consumer preferences. In the last decade, rice has become a model to validate the function of specific genes, resulting in valuable but scattered information. Here, we aimed to identify particular genes in rice related to traits that can be targeted by different mutation techniques in the breeding of crops. We selected gain of function, misfunction, and specific mutations associated with phenotypes of agronomic interest. The review includes specific trait-related genes involved in domestication, stress, herbicide tolerance, pathogen resistance, grain number, quality, weight, plant structure, nitrogen use, and others. The information presented can be used for rice and crops with similar or homologous genes to breed crops that require improvement to achieve more sustainable production in challenging farming conditions.

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Production of Two Elite Glutinous Rice Varieties by Editing Wx Gene

Cited by 33 publications

References 37 publications

Assessment of the Characteristics of Waxy Rice Mutants Generated by CRISPR/Cas9

Assessment of the Characteristics of Waxy Rice Mutants Generated by CRISPR/Cas9

Rice breeding in the new era: comparison of useful agronomic traits

Rice agronomic traits and variability induced by mutagenesis

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