Production of two morphologically different antimony trioxides by a novel antimonate-reducing bacterium, Geobacter sp. SVR

Yamamura, Shigeki; Iida, Chisato; Kobayashi, Yayoi; Watanabe, Mirai; Amachi, Seigo

doi:10.1016/j.jhazmat.2021.125100

Cited by 25 publications

(7 citation statements)

References 50 publications

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“…Compared to arrA , anrA was only detected in Geobacter sp. SVR isolated from Sb mine soil in Hyogo Prefecture, Japan. , Unlike the presence of arrA and anrA in Geobacter -associated bins identified in this study, no arrA was detected in the genome of Geobacter sp. SVR which contains anrA while no anrA was detected in Geobacter OR-1 which contains arrA .…”

Section: Discussioncontrasting

confidence: 77%

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Identification of Antimonate Reducing Bacteria and Their Potential Metabolic Traits by the Combination of Stable Isotope Probing and Metagenomic-Pangenomic Analysis

Sun

Häggblom

et al. 2021

Environ. Sci. Technol.

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Microorganisms play an important role in altering antimony (Sb) speciation, mobility, and bioavailability, but the understanding of the microorganisms responsible for Sb(V) reduction has been limited. In this study, DNA-stable isotope probing (DNA-SIP) and metagenomics analysis were combined to identify potential Sb(V)-reducing bacteria (SbRB) and predict their metabolic pathways for Sb(V) reduction. Soil slurry cultures inoculated with Sb-contaminated paddy soils from two Sb-contaminated sites demonstrated the capability to reduce Sb(V). DNA-SIP identified bacteria belonging to the genera Pseudomonas and Geobacter as putative SbRB in these two Sb-contaminated sites. In addition, bacteria such as Lysinibacillus and Dechloromonas may potentially participate in Sb(V) reduction. Nearly complete draft genomes of putative SbRB (i.e., Pseudomonas and Geobacter) were obtained, and the genes potentially responsible for arsenic (As) and Sb reduction (i.e., respiratory arsenate reductase (arrA) and antimonate reductase (anrA)) were examined. Notably, bins affiliated with Geobacter contained arrA and anrA genes, supporting our hypothesis that they are putative SbRB. Further, pangenomic analysis indicated that various Geobacter-associated genomes obtained from diverse habitats also contained arrA and anrA genes. In contrast, Pseudomonas may use a predicted DMSO reductase closely related to sbrA (Sb(V) reductase gene) clade II to reduce Sb(V), which may need further experiments to verify. This current work represents a demonstration of using DNA-SIP and metagenomic-binning to identify SbRB and their key genes involved in Sb(V) reduction and provides valuable data sets to link bacterial identities with Sb(V) reduction.

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Section: Discussioncontrasting

confidence: 77%

“…22 Recently, Geobacter sp. strain SVR was identified as SbRB isolated from an Sb mining soil located in Japan, 59,60 suggesting their capability in reducing Sb(V).…”

Section: ■ Discussionmentioning

confidence: 99%

Identification of Antimonate Reducing Bacteria and Their Potential Metabolic Traits by the Combination of Stable Isotope Probing and Metagenomic-Pangenomic Analysis

Sun

Häggblom

et al. 2021

Environ. Sci. Technol.

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“…ANA-3, Geobacter sp. SVR) can reduce Sb V -O to immobile Sb III -O, resulting in a decrease in the aqueous Sb concentration. − On the other hand, DMRB-mediated Fe(III) reduction may enhance Sb release by the dissolution of iron minerals , or re-sequester the released Sb by original and in situ formation of secondary Fe minerals (e.g. feroxyhyte and goethite). ,, …”

Section: Introductionmentioning

confidence: 99%

New Mobilization Pathway of Antimonite: Thiolation and Oxidation by Dissimilatory Metal-Reducing Bacteria via Elemental Sulfur Respiration

Zhong

Zhang

et al. 2021

Environ. Sci. Technol.

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Antimony (Sb) mobilization is widely explored with dissimilatory metal-reducing bacteria (DMRB) via microbial iron(III)-reduction. Here, our study found a previously unknown pathway whereby DMRB release adsorbed antimonite (SbIII-O) from goethite via elemental sulfur (S0) respiratory reduction under mild alkaline conditions. We incubated SbIII-O-loaded goethite with Shewanella oneidensis MR-1 in the presence of S0 at pH 8.5. The incubation results showed that MR-1 reduced S0 instead of goethite, and biogenic sulfide induced the formation of thioantimonite (SbIII-S). SbIII-S was then oxidized by S0 to mobile thioantimonate (SbV-S), resulting in over fourfold greater Sb release to water compared with the abiotic control. SbIV-S was identified as the intermediate during the oxidation process by Fourier transform ion cyclotron resonance mass spectrometry and electron spin resonance analysis. The existence of SbIV-S reveals that the oxidation of SbIII-S to SbV-S follows a two-step consecutive one-electron transfer from Sb to S atoms. SbV-S then links with SbIII-S by sharing S atoms and inhibits SbIII-S polymerization and SbIII 2S3 precipitation like a “capping agent”. This study clarifies the thiolation and oxidation pathway of SbIII-O to SbV-S by S0 respiration and expands the role of DMRB in the fate of Sb.

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“…Yamamura et al found that a new dissimilatory antimonate-reducing bacterium, Geobacter spp., was involved in antimonate(V) reduction in nature. 33 Ramlibacter was reported to be responsible for pyrene degradation in soils. 34 However, other microorganisms enriched in MFC_CPF, including Massilia, Flavitalea, and Gemmatimonas, have seldom been reported as responsible for the degradation of pollutants.…”

Section: Discussionmentioning

confidence: 99%

“…Yamamura et al found that a new dissimilatory antimonate-reducing bacterium, Geobacter spp., was involved in antimonate( v ) reduction in nature. 33 Ramlibacter was reported to be responsible for pyrene degradation in soils. 34…”

Section: Discussionmentioning

confidence: 99%

Identification of chlorpyrifos-degrading microorganisms in farmland soils via cultivation-independent and -dependent approaches

Lian

Xing

Zhang

et al. 2022

Environ. Sci.: Processes Impacts

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Production of two morphologically different antimony trioxides by a novel antimonate-reducing bacterium, Geobacter sp. SVR

Cited by 25 publications

References 50 publications

Identification of Antimonate Reducing Bacteria and Their Potential Metabolic Traits by the Combination of Stable Isotope Probing and Metagenomic-Pangenomic Analysis

Identification of Antimonate Reducing Bacteria and Their Potential Metabolic Traits by the Combination of Stable Isotope Probing and Metagenomic-Pangenomic Analysis

New Mobilization Pathway of Antimonite: Thiolation and Oxidation by Dissimilatory Metal-Reducing Bacteria via Elemental Sulfur Respiration

Identification of chlorpyrifos-degrading microorganisms in farmland soils via cultivation-independent and -dependent approaches

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