Production of Two Vaccinating Recombinant Rotavirus Proteins in the Milk of Transgenic Rabbits

Soler, Éric; Saux, Agnès Le; Guinut, Frédéric; Passet, Bruno; Cohen, Ruxandra; Merle, Christine; Charpilienne, Annie; Fourgeux, Cynthia; Sorel, Véronique; Piriou, Antoine; Schwartz-Cornil, Isabelle; Cohen, Jean; Houdebiné, Louis‐Marie

doi:10.1007/s11248-005-1771-0

Cited by 28 publications

(17 citation statements)

References 44 publications

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“…coho salmon ͉ phenotypic plasticity ͉ risk-assessment T ransgenes with a variety of functions have now been inserted into a wide range of animals with foreseeable applications ranging from small-scale basic laboratory research and applied medicine to large-scale disease control and commercial meat production (1)(2)(3)(4)(5)(6). The latter application, in particular, has raised concern regarding the potential negative impact that transgenic animals may have on the natural environment whether they escape from rearing facilities or are purposefully released into the wild (7).…”

mentioning

confidence: 99%

Gene–environment interactions influence ecological consequences of transgenic animals

Sundström

Lõhmus

Tymchuk

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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Production of transgenic animals has raised concern regarding their potential ecological impact should they escape or be released to the natural environment. This concern has arisen mainly from research on laboratory-reared animals and theoretical modeling exercises. In this study, we used biocontained naturalized stream environments and conventional hatchery environments to show that differences in phenotype between transgenic and wild genotypes depend on rearing conditions and, critically, that such genotype-by-environment interactions may influence subsequent ecological effects in nature. Genetically wild and growth hormone transgenic coho salmon (Oncorhynchus kisutch) were reared from the fry stage under either standard hatchery conditions or under naturalized stream conditions. When reared under standard hatchery conditions, the transgenic fish grew almost three times longer than wild conspecifics and had (under simulated natural conditions) stronger predation effects on prey than wild genotypes (even after compensation for size differences). In contrast, when fish were reared under naturalized stream conditions, transgenic fish were only 20% longer than the wild fish, and the magnitude of difference in relative predation effects was much reduced. These data show that genotype-by-environment interactions can influence the relative phenotype of transgenic and wild-type organisms and that extrapolations of ecological consequences from phenotypes developed in the unnatural laboratory environment may lead to an overestimation or underestimation of ecological risk. Thus, for transgenic organisms that may not be released to nature, the establishment of a range of highly naturalized environments will be critical for acquiring reliable experimental data to be used in risk assessments.coho salmon ͉ phenotypic plasticity ͉ risk-assessment

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mentioning

confidence: 99%

Gene–environment interactions influence ecological consequences of transgenic animals

Sundström

Lõhmus

Tymchuk

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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“…The proteins prepared in rabbit milk are as follows: human EPO (erythropoietin) (Massoud et al, 1996), human extracellular superoxide dismutase (EC SOD) (Strö mqvist et al, 1996), equine LH/CG (Galet et al, 2001), human inhibitor C1 (Koles et al, 2004), rotavirus VP2 and VP6 proteins to be used as vaccine (Soler et al, 2005) and alkaline phosphatase (Bodrogi et al, 2006). Several proteins were also produced in mouse milk at a high level: human a1-antitrysin (Bischoff et al, 1992), human growth hormone and bovine growth hormone (Thé pot et al, 1995).…”

Section: Use Of Transgenesismentioning

confidence: 99%

Rabbit milk protein genes: from mRNA identification to chromatin structure

Jolivet

Daniel-Carlier

Thépot

et al. 2008

Animal

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Milk protein genes are among the most intensively expressed and they are active only in epithelial mammary cells of lactating animals. They code for proteins which represent 30% of the proteins consumed by humans in developed countries. Mammary gland development occurs essentially during each pregnancy. This offers experimenters attractive models to study the expression mechanisms of genes controlled by known hormones and factors (prolactin, glucocorticoids, progesterone, insulin-like growth factor-1 and others) as well as extracellular matrix. In the mid-1970s, it became possible to identify and quantify mRNAs from higher living organisms using translation in reticulocyte lysate. A few years later, the use of radioactive cDNAs as probes made it possible for the quantification of mRNA in various physiological situations using hybridisation in the liquid phase. Gene cloning offered additional tools to measure milk protein mRNAs and also to identify transcription factors. Gene transfer in cultured mammary cells and in animals contributed greatly to these studies. It is now well established that most if not all genes of higher eukaryotes are under the control of multiple distal regulatory elements and that local modifications of the chromatin structure play an essential role in the mechanisms of differentiation from embryos to adults. The technique, known as ChIP (chromatin immunoprecipitation), is being implemented to identify the factors that modify chromatin structure at the milk protein gene level during embryo development, mammogenesis and lactogenesis, including the action of hormones and extracellular matrix. Transgenesis is not just a tool to study gene regulation and function, it is also currently used for various biotechnological applications including the preparation of pharmaceutical proteins in milk. This implies the design of efficient vectors capable of directing the secretion of recombinant proteins in milk at a high concentration. Milk protein gene promoters and long genomic-DNA fragments containing essentially all the regulatory elements of milk protein genes are used to optimise recombinant protein production in milk.Keywords: chromatin, development, promoter, transgenesis IntroductionMilk protein genes are abundantly and specifically expressed in the mammary gland of lactating mammals. Lactation is essential for the development of newborns. Since the domestication of a few ruminants, milk has become an important food for humans. Milk proteins represent 30% of the proteins consumed by humans in developed countries. On the other hand, milk protein genes are among the most intensively expressed genes and they were soon candidates to study the mechanisms of gene expression. For these reasons, biology of lactation has been extensively studied for the last 50 years. The main factors controlling mammary gland development and milk protein gene expression are well known. These factors are oestrogens, prolactin, progesterone, glucocorticoids, insulin-like growth factor-1, placental lactogen and extracellu...

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“…Human protein C inhibitor prepared in rabbit milk is in phase III of clinical studies [37]. Other proteins such as recombinant rotaviral proteins prepared in rabbit milk [34,35] could become a safe and efficient vaccine for humans and farm animals.…”

Section: Production Of Pharmaceutical Proteins In Milkmentioning

confidence: 99%

Preparation of recombinant proteins in milk to improve human and animal health

Soler¹,

Thépot²,

Rival‐Gervier³

et al. 2006

Reprod. Nutr. Dev.

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-Milk is a very abundant source of proteins for animal and human consumption. Milk composition can be modified using transgenesis, including exogenous gene addition and endogenous gene inactivation. The study of milk protein genes has provided researchers with regulatory regions capable of efficiently and specifically driving the expression of foreign genes in milk. The projects underway are aimed at modifying milk composition, improving its nutritional value, reducing mammary infections, providing consumers with antipathogen proteins and preparing purified recombinant proteins for pharmaceutical use. The present paper summarises the current progress in this field. milk / recombinant proteins / animal and human health

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Production of Two Vaccinating Recombinant Rotavirus Proteins in the Milk of Transgenic Rabbits

Cited by 28 publications

References 44 publications

Gene–environment interactions influence ecological consequences of transgenic animals

Gene–environment interactions influence ecological consequences of transgenic animals

Rabbit milk protein genes: from mRNA identification to chromatin structure

Preparation of recombinant proteins in milk to improve human and animal health

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