Production of uniform droplets using membrane, microchannel and microfluidic emulsification devices

Vladisavljević, Goran T.; Kobayashi, Isao; Nakajima, Mitsutoshi

doi:10.1007/s10404-012-0948-0

Cited by 324 publications

(284 citation statements)

References 256 publications

(235 reference statements)

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“…The membranes were fabricated by the UV-LIGA (ultraviolet lithography, electroplating, and molding) process, which involves galvanic deposition of nickel onto the template formed by photolithography. 24 The porosity of a membrane with the hexagonal pore array is given by:…”

Section: Methodsmentioning

confidence: 99%

pH-Sensitive Micelles for Targeted Drug Delivery Prepared Using a Novel Membrane Contactor Method

Laouini

Koutroumanis

Charcosset

et al. 2013

ACS Appl. Mater. Interfaces

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A novel membrane contactor method was used to produce size-controlled poly(ethylene glycol)-b-polycaprolactone (PEG-PCL) copolymer micelles composed of diblock copolymers with different average molecular weights, M n (9200 or 10 400 Da) and hydrophilic fractions, f (0.67 or 0.59). By injecting 570 L m −2 h −1 of the organic phase (a 1 mg mL −1 solution of PEG-PCL in tetrahydrofuran) through a microengineered nickel membrane with a hexagonal pore array and 200 μm pore spacing into deionized water agitated at 700 rpm, the micelle size linearly increased from 92 nm for a 5-μm pore size to 165 nm for a 40-μm pore size. The micelle size was finely tuned by the agitation rate, transmembrane flux and aqueous to organic phase ratio. An encapsulation efficiency of 89% and a drug loading of ∼75% (w/w) were achieved when a hydrophobic drug (vitamin E) was entrapped within the micelles, as determined by ultracentrifugation method. The drug-loaded micelles had a mean size of 146 ± 7 nm, a polydispersity index of 0.09 ± 0.01, and a ζ potential of −19.5 ± 0.2 mV. When drug-loaded micelles where stored for 50 h, a pH sensitive drug release was achieved and a maximum amount of vitamin E (23%) was released at the pH of 1.9. When a pH-sensitive hydrazone bond was incorporated between PEG and PCL blocks, no significant change in micelle size was observed at the same micellization conditions.

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Section: Methodsmentioning

confidence: 99%

pH-Sensitive Micelles for Targeted Drug Delivery Prepared Using a Novel Membrane Contactor Method

Laouini

Koutroumanis

Charcosset

et al. 2013

ACS Appl. Mater. Interfaces

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“…The membranes used were nickel microengineered membranes containing uniform cylindrical pores with a diameter of 5, 10, 20 or 40 µm, arranged at a uniform spacing of 80 or 200 µm. The membranes were fabricated by the UV-LIGA process, which involves galvanic deposition of nickel onto the template formed by photolithography [17]. A perfect hexagonal array of pores with a pore at the centre of each hexagonal cell can be seen in the supplementary material ( Figure S1).…”

Section: Figurementioning

confidence: 99%

Preparation of liposomes: a novel application of microengineered membranes - investigation of the process parameters and application to the encapsulation of vitamin E

et al. 2013

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Liposomes with a mean size of 59-308 nm suitable for pulmonary drug delivery were prepared by the ethanol injection method using nickel microengineered flat disc membranes with a uniform pore size of 5-40 mm and a pore spacing of 80 or 200 mm. An ethanolic phase containing 20-50 mg ml 21 phospholipid (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) or Lipoid1 E80), 5-12.5 mg ml 21 stabilizer (cholesterol, stearic acid or cocoa butter), and 0 or 5 mg ml 21 vitamin E was injected through the membrane into an agitated aqueous phase at a controlled flux of 142-355 l m 22 h 21 and a shear stress on the membrane surface of 0.80-16 Pa. The mean particle size obtained under optimal conditions was 84 and 59 nm for Lipoid E80 and POPC liposomes, respectively. The particle size of the prepared liposomes increased with an increase in the pore size of the membrane and decreased with an increase in the pore spacing. Lipoid E80 liposomes stabilized by cholesterol or stearic acid maintained their initial size within 3 months. A high entrapment efficiency of 99.87% was achieved when Lipoid E80 liposomes were loaded with vitamin E. Transmission electron microscopy images revealed spherical multi-lamellar structure of vesicles. The reproducibility of the developed fabrication method was high.

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“…23,24 Micropatterning and microfluidic systems have been used to create hydrogels with spatially controlled organization as well as numerous cellmaterial or cell-cell combinations to analyze distinct biological issues. 25,26 Micro-patterned arrays have been generated by immobilizing hydrogels on different micro-domains within a plane surface, and they have been used for disease diagnosis, prognosis, biochemical analysis, and therapeutic regimes, and have become an alternative approach for high-throughput multiplexed assays. [27][28][29][30] Strategies avoiding such 2D fixation of various hydrogels have employed shape-coded hydrogel particles as a suspension microarray format for multiplexed bioassays.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Hydrogel Particles of Defined Shapes Using Superhydrophobic‐Hydrophilic Micropatterns

et al. 2016

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We report a method to rapidly fabricate alginate hydrogel particles of specific sizes and shapes.Our method is based on the formation of arrays of droplets of pre-hydrogel solutions on superhydrophobic-hydrophilic patterns using the process of discontinuous dewetting, followed by their gelation via the parallel addition of CaCl 2 to the individual droplets via the sandwiching method. We demonstrate that viability of living cells incorporated within the hydrogel particles is higher during the long-term cultivation than in the case of cells cultured in the bulk threedimensional hydrogel matrix. Incorporation of magnetic particles into the free-standing hydrogel particles containing living cells enabled ease manipulation of the particles using an external magnetic field.

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Production of uniform droplets using membrane, microchannel and microfluidic emulsification devices

Cited by 324 publications

References 256 publications

pH-Sensitive Micelles for Targeted Drug Delivery Prepared Using a Novel Membrane Contactor Method

pH-Sensitive Micelles for Targeted Drug Delivery Prepared Using a Novel Membrane Contactor Method

Preparation of liposomes: a novel application of microengineered membranes - investigation of the process parameters and application to the encapsulation of vitamin E

Fabrication of Hydrogel Particles of Defined Shapes Using Superhydrophobic‐Hydrophilic Micropatterns

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