Production of Virus-free Plants

Walkey, D. G. A.

doi:10.1007/978-94-011-3090-5_11

Cited by 9 publications

(1 citation statement)

References 40 publications

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“…Tissue culture techniques such as meristem tip culture, chemotherapy and thermotherapy either alone or in combination have been proved to be successful in producing virus-free plants. Dahlia, freesia, geranium, lily, etc., have been raised virus-free using shoot tip meristems (Paludan 1973, Walkey 1991. Thermotherapy along with meristem tip culture has been used to raise virus-free carnation, narcissus, chrysanthemum and others (Hakkaart and Quak 1964).…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Indexing and production of virus-free chrysanthemums

Ram¹,

Verma²,

Singh³

et al. 2005

Biologia plant.

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Chrysanthemum morifolium cv. Regol Time plants were found to be infected with Chrysanthemum B carlavirus (CVB). They were made CVB-free by using meristem tip culture, chemotherapy and thermotherapy. The plants were indexed by biological assay, double antibody sandwitch enzyme linked immunosorbent assay (DAS-ELISA) and reverse transcriptase polymerase chain reaction (RT-PCR). The maximum number of virus-free plants (26.7 %, as indexed by RT-PCR) was obtained with 2-thiouracil at 0.04 g dm -3 concentration. Only 12 % plants were found to be virus-free, after being kept at 38 °C for 30 d. For indexing CVB in chrysanthemums, RT-PCR was found to be the most reliable method.Additional key words: antiviral compounds, Chrysanthemum B carlavirus, Chrysanthemum morifolium, DAS-ELISA, in vitro production, RT-PCR.

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Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Indexing and production of virus-free chrysanthemums

Ram¹,

Verma²,

Singh³

et al. 2005

Biologia plant.

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In Vitro Elimination of Prunus Necrotic Ringspot Virus in a Plum Cultivar

Spiegel

Tam

Rosner

et al. 1999

Plant Biotechnology and in Vitro Biology in the 21st Century

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Some like it hot: citrus tristeza virus strains react differently to elevated temperature

2016

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Viruses often infect plants as a mixed population. The dynamics of viral populations dictate the success of the infection, yet there is little understanding of the factors that influence them. It is known that temperature can affect individual viruses; could it also affect a virus population? In order to study this, we observed citrus tristeza virus (CTV) populations in different hosts under winter and summer conditions (25 versus 36 °C). We found that only some CTV strains were affected by a higher summer temperature, which lead to a change in CTV population structure, and that this effect was host dependent.

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Production of Virus-free Plants

Cited by 9 publications

References 40 publications

Indexing and production of virus-free chrysanthemums

Indexing and production of virus-free chrysanthemums

In Vitro Elimination of Prunus Necrotic Ringspot Virus in a Plum Cultivar

Some like it hot: citrus tristeza virus strains react differently to elevated temperature

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