Production of volatile compounds by yeasts using hydrolysed grape seed oil obtained by immobilized lipases in continuous packed-bed reactors

Castiglioni, Gabriele Zanota; Bettio, Giulia; Matte, Carla Roberta; Jacques, Rosângela Assis; Polidoro, Allan dos S.; Rosa, Carlos A.; Ayub, Marco Antônio Záchia

doi:10.1007/s00449-020-02334-4

Cited by 8 publications

(7 citation statements)

References 47 publications

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“…For grape seed oil samples, a moderate increase ( p > 0.05) in the amount of free unsaturated fatty acids was recorded during the lipolysis ( Figure 1 F). However, it was shown that the major fatty acids found in grape seed oil triglycerides [ 7 , 36 ], i.e., LA and OA, can be released by the R. miehei lipase treatment conducted. In addition, it is worth mentioning the significant ( p < 0.0001) improvement in free PA content of the grape seed oil sample after hydrolysis ( Figure 1 F).…”

Section: Resultsmentioning

confidence: 99%

“…The majority of bioactive fatty acids in natural oils are usually bounded in triglycerides, which reduces their bioavailability [ 32 ]. These compounds can be obtained from the oil through sub- and supercritical water extraction [ 33 ], or acidic, alkaline or enzyme-assisted hydrolysis reactions [ 34 , 35 , 36 ]. The lipase-assisted hydrolysis has reported to be as an ecofriendly approach for the extraction.…”

Section: Introductionmentioning

confidence: 99%

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Hydrolysis of Edible Oils by Fungal Lipases: An Effective Tool to Produce Bioactive Extracts with Antioxidant and Antimicrobial Potential

Kotogán

Furka

Kovács

et al. 2022

Foods

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Hydrolysis of olive, rapeseed, linseed, almond, peanut, grape seed and menhaden oils was performed with commercial lipases of Aspergillus niger, Rhizopus oryzae, Rhizopus niveus, Rhizomucor miehei and Candida rugosa. In chromogenic plate tests, olive, rapeseed, peanut and linseed oils degraded well even after 2 h of incubation, and the R. miehei, A. niger and R. oryzae lipases exhibited the highest overall action against the oils. Gas chromatography analysis of vegetable oils hydrolyzed by R. miehei lipase revealed about 1.1 to 38.4-fold increases in the concentrations of palmitic, stearic, oleic, linoleic and α-linolenic acids after the treatment, depending on the fatty acids and the oil. The major polyunsaturated fatty acids produced by R. miehei lipase treatment from menhaden oil were linoleic, α-linolenic, hexadecanedioic, eicosapentaenoic, docosapentaenoic and docosahexaenoic acids, with yields from 12.02 to 52.85 µg/mL reaction mixture. Folin–Ciocalteu and ferric reducing power assays demonstrated improved antioxidant capacity for most tested oils after the lipase treatment in relation to the concentrations of some fatty acids. Some lipase-treated and untreated samples of oils, at 1.25 mg/mL lipid concentration, inhibited the growth of food-contaminating bacteria. The lipid mixtures obtained can be reliable sources of extractable fatty acids with health benefits.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hydrolysis of Edible Oils by Fungal Lipases: An Effective Tool to Produce Bioactive Extracts with Antioxidant and Antimicrobial Potential

Kotogán

Furka

Kovács

et al. 2022

Foods

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show abstract

“…72 The drying step also helps to remove volatile residues that could interfere with the reaction, that may induce additional unwanted reactions that may take up the enzyme's active sites. 33 In addition, Lipozyme RM IM was used in the synthesis of human milk fat-style structured triglycerides, and it was found that the biocatalyst could be reused up to eight runs in a batch reactor. 21 4.2.2.…”

Section: Type Of Reactors Used and Choice Of Enzymes In Biocatalysismentioning

confidence: 99%

“…Factors such as temperature and agitation of the reactor affect the amount of time taken for reaction. The residence time in biocatalysis ranges from 1 to 8 h. ,,,− …”

Section: Overview Of Reaction Conditionsmentioning

confidence: 99%

Enzyme Biocatalysis for Sustainability Applications in Reactors: A Systematic Review

Chook,

Aroua,

Gew

2023

Ind. Eng. Chem. Res.

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Enzyme biocatalysis is well known for its biodegradability and environmentally friendly conditions. Its novel properties such as high reaction selectivity and mild reaction conditions prove that it is a promising alternative that can overcome the global resource scarcity of the conventional chemical catalysts that are of a nonrenewable source. In this study, we documented the advancements and breakthroughs in emerging biocatalysis reactor technologies spanning over a decade. We aim to encapsulate the remarkable progress and innovations witnessed within this dynamic field. Thus, we reviewed 48 articles related to enzyme biocatalysis in the production of glycerides using reactors. The reaction parameters using a biocatalysis route were reviewed systematically and discussed in our study. In the biocatalysis transformation, the processes mainly focus on the conversion of raw material into product. In the optimization of biocatalysis, temperature and residence time parameters have been thoroughly researched in order to maximize the productivity of the enzyme activity. In the selection of reactors, the type of reactor and its agitator play a vital role in enzyme activity. We anticipate that the greenness of biocatalysis transformation could be improved if the enzymes in waste streams could be converted from another reaction to functional products. This work may serve as a stepping stone for the biocatalysis industry, acting as a precursor for industries or individuals interested in exploring this evergreen field.

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“…Recently, the main innovation trend in food industry has been the use of biotechnology techniques to produce additives such as colorants and flavorings, 1‐3 besides the production of vitamins, enzymes, prebiotics, and probiotics, aiming to improve the nutritional and sensory properties of foods and beverages. Consumers market demand and changes in purchasing and consumption behavior are the main factors that explain this scenario, among the increasing concern to minimize waste generation 4 and to support eco‐friendly supply chains 5 …”

Section: Introductionmentioning

confidence: 99%

Bioconversion of ferulic acid into aroma compounds by newly isolated yeast strains of the Latin American biodiversity

Bettio

Zardo

Rosa

et al. 2020

Biotechnology Progress

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Nine yeast strains isolated from Latin American biodiversity were screened for ferulic acid (FA) consumption and conversion into aroma compounds such as vanillin, vanillic acid (VA), and 4-vinylguaiacol (VG). Selected strains (Rhodotorula mucilaginosa UFMG-CM-Y3647, UFMG-CM-Y2190, UFMG-CM-Y665) were evaluated in flask experiments to investigate the influence of the pH media on bioconversion and a two-step process was conducted to maximize the metabolites production. The effect of pH was found to be significantly important for FA bioconversion, as acidic conditions (pH < 6.0) improved VA accumulation, with highest production of 1.14 ± 0.02 and 1.25 ± 0.03 g/L shown by UFMG-CM-Y3647 and UFMG-CM-Y2190, respectively. The two-step process favored 4-VG production for most strains, being UFMG-CM-Y2190 the best producer, its cultures reaching 1.63 ± 0.09 g/L after 55 hr, showing a productivity of 29.59 ± 1.55 mg/(LÁhr), as glucose affected the metabolites pool and redirected yeast metabolism. R mucilaginosa UFMG-CM-Y3647 was selected for scaled-up cultivations in a 2-L bioreactor, where pH-controlled pH 5.5 and aeration of 2.5 vvm was found to be the best condition to improve VA productivity, attaining final concentrations of 1.20 ± 0.02 g/L −1 (78% molar yield) and a productivity of 40.82 ± 0.57 mg/(LÁhr).

show abstract

Production of volatile compounds by yeasts using hydrolysed grape seed oil obtained by immobilized lipases in continuous packed-bed reactors

Cited by 8 publications

References 47 publications

Hydrolysis of Edible Oils by Fungal Lipases: An Effective Tool to Produce Bioactive Extracts with Antioxidant and Antimicrobial Potential

Hydrolysis of Edible Oils by Fungal Lipases: An Effective Tool to Produce Bioactive Extracts with Antioxidant and Antimicrobial Potential

Enzyme Biocatalysis for Sustainability Applications in Reactors: A Systematic Review

Bioconversion of ferulic acid into aroma compounds by newly isolated yeast strains of the Latin American biodiversity

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