Production of welan gum from cane molasses by Sphingomonas sp. FM01

Li, Qiwen; Zhou, Yuan; Ke, Chengzhu; Bai, Yunxia; Liu, Xiaoling; Li, Shubo

doi:10.1016/j.carbpol.2020.116485

Cited by 38 publications

(21 citation statements)

References 33 publications

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“…The pretreatment of molasses is required to remove the impurities and hydrolyze the sucrose to its monomer glucose and fructose before being used as the substrate. 29 The sucrose, glucose, and fructose account for 79.4%, 12.5%, and 8.2%, respectively, of sugar components in the diluted molasses used in the present study with a total carbohydrate content of 264.32 g/L (Figure 6). The treatment of molasses with the MHA method accelerated the hydrolysis of sucrose, and the proportion of available D-glucose and Dfructose reached 44.1% (122.88 g/L) and 41.5% (115.64 g/L), respectively.…”

Section: Resultsmentioning

confidence: 75%

Reconstruction of a Cofactor Self-Sufficient Whole-Cell Biocatalyst System for Efficient Biosynthesis of Allitol from d-Glucose

Zhao

Guo

et al. 2022

J. Agric. Food Chem.

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The combined catalysis of glucose isomerase (GI), d-psicose 3-epimerase (DPEase), ribitol dehydrogenase (RDH), and formate dehydrogenase (FDH) provides a convenient route for the biosynthesis of allitol from d-glucose; however, the low catalytic efficiency restricts its industrial applications. Here, the supplementation of 0.32 g/L NAD+ significantly promoted the cell catalytic activity by 1.18-fold, suggesting that the insufficient intracellular NAD(H) content was a limiting factor in allitol production. Glucose dehydrogenase (GDH) with 18.13-fold higher activity than FDH was used for reconstructing a cofactor self-sufficient system, which was combined with the overexpression of the rate-limiting genes involved in NAD+ salvage metabolic flow to expand the available intracellular NAD(H) pool. Then, the multienzyme self-assembly system with SpyTag and SpyCatcher effectively channeled intermediates, leading to an 81.1% increase in allitol titer to 15.03 g/L from 25 g/L d-glucose. This study provided a facilitated strategy for large-scale and efficient biosynthesis of allitol from a low-cost substrate.

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Section: Resultsmentioning

confidence: 75%

Reconstruction of a Cofactor Self-Sufficient Whole-Cell Biocatalyst System for Efficient Biosynthesis of Allitol from d-Glucose

Zhao

Guo

et al. 2022

J. Agric. Food Chem.

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“…Meanwhile, after submerged cultivation, extraction, and purification, a polysaccharide (CGP-SM) was obtained (Figure 1C) and its yield was 5.83 ± 0.09 g/l (Table 1), the color of CGP-SM might be due to the pigments in which it was left. This yield is lower than welan gum (37.65 g/l) (14) and sophorolipid (53 ± 3 g/l) (24) produced from molasses, but higher than chitosan (0.39 g/l) (25) and hyaluronic acid (3.48 g/l) (4) produced from molasses with submerged cultivation. Many factors, such as microbial species, culture conditions, and nutritional types, could affect polysaccharide yields (26), but submerged cultivation has the advantages of better controlling cultivation conditions, less pollution, less time consumption, high efficiency, and less space occupation.…”

Section: Polysaccharide Productionmentioning

confidence: 78%

“…( 12 ). Meanwhile, many polysaccharides have also been produced from molasses, including pullulan ( 13 ), welan gum ( 14 ), hyaluronic acid ( 4 ), and glucan ( 3 ). The above research provided help for the production of polysaccharides from low-cost molasses with an environmentally microbial process.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Antibacterial Activity of a Polysaccharide Produced From Sugarcane Molasses by Chaetomium globosum CGMCC 6882

Guo

Yang

et al. 2022

Front. Nutr.

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As a by-product of the sugar industry containing many sugars, proteins, nitrogenous materials, and heavy metals, molasses is rarely used for polysaccharide production. In the present work, a Chaetomium globosum CGMCC 6882 polysaccharide was produced from sugarcane molasses (CGP-SM) was successfully produced from sugarcane molasses. The yield of CGP-SM was 5.83 ± 0.09 g/l and its protein content was 2.41 ± 0.12% (w/w). Structural analysis showed that CGP-SM was a crystalline and amorphous polysaccharide containing rhamnose, glucosamine, galactose, glucose, mannose, fructose, and glucuronic acid in the molar ratio of 10.31: 1.14: 2.07: 59.55: 42.65: 1.92: 9.63. Meanwhile, weight-average molecular weight (Mw), number-average molecular weight (Mn), and polydispersity (Mw/Mn) of CGP-SM were 28.37 KDa, 23.66 KDa, and 1.199, respectively. Furthermore, the bacteriostatic assay indicated that CGP-SM inhibited the growth of Escherichia coli and Staphylococcus aureus in a concentration-dependent manner, and its inhibitory effect on S. aureus was higher than that of E. coli. Above all, this work provides a green method for the production of bioactive polysaccharide from sugarcane molasses.

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“…The phenomenon was consistent with the previous reported results that high molasses concentration showed significant inhibition of cell growth and products synthesis [ 17 , 31 , 32 ]. When γ-PGA producers were cultured in medium with high concentration of molasses, the inhibitors in molasses exceeded the tolerance limit of strains [ 33 ]. Furthermore, high concentration of sugar may also show inhibitory effects on cell growth [ 17 ].…”

Section: Resultsmentioning

confidence: 99%

Efficient molasses utilization for low-molecular-weight poly-γ-glutamic acid production using a novel Bacillus subtilis strain

Chen

et al. 2022

Microb Cell Fact

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Background Poly-γ-glutamic acid (γ-PGA) is a biopolymer and has various applications based on its biocompatibility, non-toxicity, and edibility. Low-molecular-weight (Mw)-γ-PGA has promising applications in agriculture and pharmaceuticals. It is traditionally produced by enzymatic hydrolysis. Cost-effective bioproduction of low-Mw-γ-PGA is essential for commercial application of γ-PGA. Results Bacillus subtilis 242 is a newly isolated low-Mw-γ-PGA-producing strain. To develop cost-effective production of γ-PGA using this newly isolated strain, cane molasses and corn steep liquor were used to produce γ-PGA. The concentration of cane molasses was optimized and 100 g/L cane molasses resulted in high γ-PGA production. The effects of yeast extract and corn steep liquor on γ-PGA yield were investigated. High concentration of γ-PGA was obtained in the medium with corn steep liquor. A concentration of 32.14 g/L γ-PGA was achieved in fed-batch fermentation, with a productivity of 0.67 g/L/h and a percentage yield (gγ-PGA/gglutamate) of 106.39%. The Mw of γ-PGA was 27.99 kDa. Conclusion This study demonstrated the potential application of B. subtilis 242 for cost-effective production of low-Mw-γ-PGA from cane molasses.

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Production of welan gum from cane molasses by Sphingomonas sp. FM01

Cited by 38 publications

References 33 publications

Reconstruction of a Cofactor Self-Sufficient Whole-Cell Biocatalyst System for Efficient Biosynthesis of Allitol from d-Glucose

Reconstruction of a Cofactor Self-Sufficient Whole-Cell Biocatalyst System for Efficient Biosynthesis of Allitol from d-Glucose

Characterization and Antibacterial Activity of a Polysaccharide Produced From Sugarcane Molasses by Chaetomium globosum CGMCC 6882

Efficient molasses utilization for low-molecular-weight poly-γ-glutamic acid production using a novel Bacillus subtilis strain

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