Abstract:Xylanase is an important enzyme with potential application in the degradation of xylan component in the lignocellulosic biomass. There are very few reports on the production of cellulase free xylanases especially by yeast strains which have great potential in paper and pulp industry in removing the hemicellulose from the treated or untreated pulp. In this study, P. hubeiensis NCIM 3574 isolated in our laboratory produced significant levels of extracellular cellulase free xylanase (2480 IU/g DSS) in solid state… Show more
“… 40 So cocktail of commercially available biomass degrading enzymes must be supplemented with microbial enzymes in order to attain the full potential of plant biomass in industrial application. 41 The aim of this study was to purify and characterize the xylosidase gene of C. clariflavum expressed in E. coli BL21 (DE3) using the pET-21a(+) expression vector for use in industrial applications.…”
The present study describes the cloning, expression, purification and characterization of a xylosidase gene from Clostridium clariflavum into E. coli BL21 (DE3) using the expression vector pET-21a(+) for utilization in biofuel production.
“… 40 So cocktail of commercially available biomass degrading enzymes must be supplemented with microbial enzymes in order to attain the full potential of plant biomass in industrial application. 41 The aim of this study was to purify and characterize the xylosidase gene of C. clariflavum expressed in E. coli BL21 (DE3) using the pET-21a(+) expression vector for use in industrial applications.…”
The present study describes the cloning, expression, purification and characterization of a xylosidase gene from Clostridium clariflavum into E. coli BL21 (DE3) using the expression vector pET-21a(+) for utilization in biofuel production.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.