Production of xylanase by Aspergillus awamori on synthetic medium in shake flask cultures

Siedenberg, D.; Gerlach, Stephanie; Schügerl, K.; Giuseppin, Marco L. F.; Hunik, J.H.

doi:10.1016/s0032-9592(97)00090-3

Cited by 21 publications

(6 citation statements)

References 20 publications

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“…The results obtained with xylan as carbon substrate, however, demonstrated that the relatively high optimal pH for xylanase production in the Q P max maximum volumetric rate of xylanase production, calculated from the maximum slope of the enzyme activity versus time curve, Y p/x specific xylanase yield, units per biomass, l max maximum specific growth rate J Ind Microbiol Biotechnol (2008) 35:587-594 591 SSL-based medium was not solely due to the greater inhibition by acetic acid at the lower pH values. Some reports indicated that xylose was a good inducer of xylanase production [10,24], whereas others found that xylose repressed xylanase production [14,32], suggesting that xylose repression of xylanase synthesis was strain specific. However, repression versus induction might also be dependent on the xylose concentration.…”

Section: Discussionmentioning

confidence: 99%

Effect of cultivation pH and agitation rate on growth and xylanase production by Aspergillus oryzae in spent sulphite liquor

Chipeta

Preez

Christopher³

2008

J Ind Microbiol Biotechnol

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The effects of cultivation pH and agitation rate on growth and extracellular xylanase production by Aspergillus oryzae NRRL 3485 were investigated in bioreactor cultures using spent sulphite liquor (SSL) and oats spelts xylan as respective carbon substrates. Xylanase production by this fungus was greatly affected by the culture pH, with pH 7.5 resulting in a high extracellular xylanase activity in the SSL-based medium as well as in a complex medium with xylan as carbon substrate. This effect, therefore, was not solely due to growth inhibition at the lower pH values by the acetic acid in the SSL. The xylanase activity in the SSL medium peaked at 199 U ml(-1) at pH 7.5 with a corresponding maximum specific growth rate of 0.39 h(-1). By contrast, the maximum extracellular beta-xylosidase activity pf 0.36 U ml(-1) was recorded at pH 4.0. Three low molecular weight xylanase isozymes were secreted at all pH values within the range of pH 4-8, whereas cellulase activity on both carbon substrates was negligible. Impeller tip velocities within the range of 1.56-3.12 m s(-1) had no marked effect, either on the xylanase activity, or on the maximum volumetric rate of xylanase production. These results also demonstrated that SSL constituted a suitable carbon feedstock as well as inducer for xylanase production in aerobic submerged culture by this strain of A. oryzae.

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Section: Discussionmentioning

confidence: 99%

Effect of cultivation pH and agitation rate on growth and xylanase production by Aspergillus oryzae in spent sulphite liquor

Chipeta

Preez

Christopher³

2008

J Ind Microbiol Biotechnol

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“…On pellet morphology, according to the report by Siedenberg et al [22] and Sitanggang et al [13], the cells of small pellet morphology consumed glucose and fructose faster than big pellet. Therefore, biomass concentration using small pellets culture would be higher than that using big pellets.…”

Section: Morphology In Fermenter Culturementioning

confidence: 94%

“…Calcium carbonate normally is as a neutralizer, prevents pH from dropping into low pH range of 2-3 which is not favorable for the biomass accumulation [22]. But in fact, calcium carbonate is not only a neutralizer; it also has significant influences on fungal pellet size.…”

Section: Effect Of Calcium Carbonatementioning

confidence: 99%

Effect of Oxygen Transfer and Pellet Size for Producing of Glucosamine Using Aspergillus sydowii BCRC 31742 Cultivated in a Fermenter

Hs¹,

Lin²

2017

J Food Process Technol

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“…When pH was 7.0 or 8.0, the fungal growth rate was rapid within preinoculation, but later slowed down. It might indicate that pH changed the charge of membrane, leading to abnormal variations of membrane osmotic pressure [12,13].…”

Section: Effects Of Fermentation Phmentioning

confidence: 99%

Enhanced Glucosamine Production with Actinomucor elegans Based on Stimulating Factor of Methanol

Liang

et al. 2014

Indian J Microbiol

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Glucosamine (GlcN) is a major and valuable component in the cell wall of fungi. In this study, the cell wall was treated via a two-stage alkali and acid process, and chitin and chitosan were fully deacetylated, partially depolymerized, and converted to GlcN oligosaccharides. Then, the oligosaccharides were analyzed by high performance liquid chromatography. The influences of Actinomucor elegans on GlcN production in a flask culture were investigated to achieve an optimum yield of GlcN. The experimental result showed that cultivation in condition of pH 6.0, 100 mL working volume (500 mL flask), 10 % (v/v) inoculum concentration, at 28 °C and 200 rpm for 6 days yielded highest dry cell weight (DCW) which was 23.43 g L(-1), with a GlcN concentration of 5.12 g L(-1). Methanol as stimulating factor was found to exert the best effect in concentration of 1.5 % (v/v). With addition of methanol into medium, the DCW increased from 23.69 to 32.42 g L(-1), leading to maximum GlcN concentration of 6.85 g L(-1) obtained. Here, the methanol addition may be useful for industrial production of GlcN, and may also be meaningful for the production of other fine chemicals by filamentous fungi.

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Production of xylanase by Aspergillus awamori on synthetic medium in shake flask cultures

Cited by 21 publications

References 20 publications

Effect of cultivation pH and agitation rate on growth and xylanase production by Aspergillus oryzae in spent sulphite liquor

Effect of cultivation pH and agitation rate on growth and xylanase production by Aspergillus oryzae in spent sulphite liquor

Effect of Oxygen Transfer and Pellet Size for Producing of Glucosamine Using Aspergillus sydowii BCRC 31742 Cultivated in a Fermenter

Enhanced Glucosamine Production with Actinomucor elegans Based on Stimulating Factor of Methanol

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