Production of xylanase by Fusarium oxysporum using agro-industrial waste

Abstract

This study aimed to optimize the production of xylanase, under submerged conditions using Fusarium oxysporum. Firstly, it was carried out the selection of the best source of carbon from agricultural waste, such as soybean husk, cassava peel, pineapple crown, corn straw, rice husk, bacaba, barley bagasse and, corncob. They were milled and sieved with a maximum granulation of 1 mm. The cultures were carried out by 132 hours 30 °C and 180 rpm. Among the agroindustrial residues, corn straw was the one that stood out with a greater enzymatic activity of 12.6 U/mL. In the second step, it was used Plackett-Burman design to screen the nutrients sources important to xylanase production. Thus, independent variables significant were urea and MgSO4. These variables selected by Plackett-Burman were then used in a Central Composite Rotational Design present activity of 26.6 U/mL and the predicted was 34.5 U/mL. So, the xylanase production by F. oxysporum can be optimize using corn straw, a low-cost waste found in large quantities.

Production of xylanase by Aspergillus niger GIO and Bacillus megaterium through solid-state fermentation

“…Bastos et al . [ 11 ] used different agro-wastes (corn cob, barley bagasse, bacaba, rice husk, corn straw, pineapple crown, cassava husk and soybean husk) for the production of xylanase. Peach-palm waste was used for the production of xylanase by Carvalho et al .…”

“…Thus there is a need for alternative substrates that will contain xylan at a low cost. Lignocellulosic substrates such as agro-wastes can be used as alternatives to xylan in the production of xylanase because these substrates contain xylan and other nutrients necessary for the growth of micro-organisms [10, 11]. Many micro-organisms such as Bacillus spp.…”