Production of Xylanase fromArthrobactersp. MTCC 6915 Using Saw Dust As Substrate under Solid State Fermentation

Sevanan, Murugan; Arnold, Donna; Pongiya, Uma Devi; Narayanan, P. M.

doi:10.4061/2011/696942

Cited by 35 publications

(20 citation statements)

References 32 publications

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“…Sepahy et al [52] reported a fermentation period of 48 h by Bacillus mojavensis AG137 in submerged fermentation using oat bran as a substrate. Murugan et al [72] reported 96 h as optimum fermentation period for xylanase production by Arthrobacter sp. MTCC 6915 in SSF using sawdust as a substrate.…”

Section: Discussionmentioning

confidence: 99%

Optimization of Cellulase and Xylanase Production by Micrococcus Species under Submerged Fermentation

et al. 2016

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This paper reports on the optimization of culture conditions for cellulase and xylanase production by bacterial isolate from lignocellulosic biomass. The bacterial isolate was screened for cellulase and xylanase production on carboxyl methyl cellulose (CMC) and birch wood xylan as substrates, respectively. One bacterial isolate showing the highest halo zone diameter (isolate PLY1) was selected for detailed studies. The analysis of the 16S ribosomal ribonucleic acid (rRNA) gene nucleotide sequence of PLY1 revealed it to have 98% similarity to Micrococcus luteus strain Fse9 and the sequence was deposited in the GenBank as Micrococcus luteus strain SAMRC-UFH3 with accession number KU171371. Cellulase production was achieved in the presence of CMC (1% w/v) under an incubation temperature of 25 • C (198 U/mL), pH 5 (173 U/mL), agitation speed 50 rpm (173 U/mL) and incubation period of 96 h (102 U/mL). Xylanase was produced maximally when birch wood xylan (1% w/v) was used as the substrate at 25 • C (1007 U/mL), pH 10 (2487 U/mL), 200 rpm (1814 U/mL), and under an incubation period of 84 h (1296 U/mL). Our findings showed that Micrococcus sp. SAMRC-UFH3 appears to be a potentially important candidate for lignocellulosic waste degradation and other relevant industrial applications.

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Section: Discussionmentioning

confidence: 99%

Optimization of Cellulase and Xylanase Production by Micrococcus Species under Submerged Fermentation

et al. 2016

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“…Thus, various agro-industrial waste materials like corn meal, corn cob, wheat bran, wheat straw, rice straw, sugarcane bagasse and coffee by-products are being used as substrates for the production of fungal xylanases (MURUGAN et al, 2011). Brewer's spent grain (BSG) is the main residue of brewing industry, representing around 85% of total by products generated (ALIYU; BALA, 2011).…”

Section: Introductionmentioning

confidence: 99%

Production of xylanases by an Aspergillus niger strain in wastes grain

Izidoro¹,

Knob²

2014

Acta Sci. Biol. Sci.

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ABSTRACT. Many fungi are used in order to extract products from their metabolism through bioprocesses capable of minimizing adverse effects caused by agro-industrial wastes in the environment. The aim of this study was to evaluate the xylanase production by an Aspergillus niger strain, using agroindustrial wastes as substrate. Brewer's spent grain was the best inducer of xylanase activity. Higher levels of xylanase were obtained when the fungus was grown in liquid Vogel medium, pH 5.0, at 30ºC, during 5 days. The temperature for optimum activity was 50ºC and optimum pH 5.0. The enzyme was stable at 50ºC, with a half-life of 240 min. High pH stability was verified from pH 4.5 to 7.0. These characteristics exhibited by A. niger xylanase turn this enzyme attractive for some industrial applications, such as in feed and food industries. Additionally, the use of brewer's spent grain, an abundantly available and low-cost residue, as substrate for xylanase production can not only add value and decrease the amount of this waste, but also reduce xylanase production cost.Keywords: agro-industrial waste, biochemical properties, filamentous fungi, xylanolytic enzymes. Produção de xilanases por uma linhagem de Aspergillus niger em resíduos de grãosRESUMO. Muitos fungos são utilizados com a finalidade de extrair produtos de seu metabolismo, por meio de bioprocessos capazes de minimizar efeitos nocivos que resíduos agroindustriais causam ao meio ambiente. O objetivo deste estudo foi avaliar a produção de xilanases por uma linhagem de Aspergillus niger, empregando resíduos agroindustriais como substrato. O bagaço de malte foi o melhor resíduo indutor da atividade xilanásica. Maiores níveis de xilanases foram obtidos quando o fungo foi cultivado em meio líquido de Vogel, pH 5,0, a 30ºC, durante cinco dias. A temperatura ótima estabelecida para a atividade xilanásica foi a de 50ºC e o pH ótimo 5,0. A enzima foi estável a 50ºC, apresentando uma meia vida de 240 min. Elevada estabilidade enzimática foi verificada entre os pH 4,5 e 7,0. As características bioquímicas exibidas pela xilanase produzida por A. niger tornam esta enzima atraente para determinadas aplicações industriais, como as indústrias de ração animal e alimentícia. Adicionalmente, a utilização do bagaço de malte, um resíduo disponível em abundância e de baixo custo como substrato para a produção de xilanases poderá não somente adicionar valor a este resíduo, como também reduzir os custos de produção destas enzimas.Palavras-chave: resíduos agroindustriais, propriedades bioquímicas, fungos filamentosos, enzimas xilanolíticas.

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“…Besides minimising the expensive costs of pure xylan and reducing the costs of dewatering system, SsF with agricultural residuals as the carbon source would remarkably result in the shortest production time possible and thus, lessen the risk of contamination, making SsF, a promising technology for xylanase production in the future. Interestingly, based on our findings, the maximum xylanase synthesis of 8.6382 U/ml was attained in the shorter fermentation time of 48 h under SsF compared to other studies which required 96 h of SsF for the maximum production of xylanase by Arthrobacter spp [63].…”

Section: Medium Ph Profile From Various Nitrogen Concentrationsmentioning

confidence: 68%

Bioprocessing of Agricultural Residuals for the Optimum Production of Extracellular Xylanase by Aspergillus brasiliensis in Solid State Fermentation (SsF)

Sali²

2014

J Biodivers Biopros Dev

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Production of Xylanase fromArthrobactersp. MTCC 6915 Using Saw Dust As Substrate under Solid State Fermentation

Cited by 35 publications

References 32 publications

Optimization of Cellulase and Xylanase Production by Micrococcus Species under Submerged Fermentation

Optimization of Cellulase and Xylanase Production by Micrococcus Species under Submerged Fermentation

<b>Production of xylanases by an <i>Aspergillus niger</i> strain in wastes grain

Bioprocessing of Agricultural Residuals for the Optimum Production of Extracellular Xylanase by Aspergillus brasiliensis in Solid State Fermentation (SsF)

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