Production of α‐amylase by <i>Aspergillus oryzae</i> As 3951 in solid state fermentation using spent brewing grains as substrate

Xu, Huaxi; Sun, Luyang; Zhao, Daqing; Zhang, Bin; Shi, Yanjun; Yahua, Wu

doi:10.1002/jsfa.3118

Cited by 31 publications

(17 citation statements)

References 34 publications

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“…BSG, which was used in this study, was also observed to be a good substrate for fungal amyloglucosidase production. Xu et al (2008) concluded that impressive amount of alpha amylase was produced from Aspergillus oryzae with solid culture cultivation method on BSG. Solid-state fermentation favored the production of amyloglucosidase significantly in cassava peels and BSG (p<0.05).…”

Section: Effect Of Wastes On Amyloglucosidase Production By Fungal Ismentioning

confidence: 98%

Production of Fungal β-amylase and Amyloglucosidase on Some Nigerian Agricultural Residues

Adeniran

Abiose

Ogunsua

2008

Food Bioprocess Technol

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Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Helminthosporium oxysporium, and Penicillium frequestans isolated from common Nigerian agricultural residues like cassava, yam, banana, and plantain peels and brewery spent grains (BSG) were screened for their ability to produce β-amylase and amyloglucosidase using submerged and solid-state cultivation regimens. Enzyme activity (EU) was determined by estimating the amount of reducing sugars produced as a result of the action of crude enzyme solutions on buffered starch solution. Results showed that A. niger liberated the highest level of β-amylase (33.2 EU) on plantain peels medium using the static cultivation method, while it produced the highest amount of amyloglucosidase (29.8 EU) on yam peels substrate with solid-state cultivation regimen. Plantain peels favored β-amylase production more than the other tested wastes supporting production of 33.2 and 9.8 EU of the enzyme in A. niger under solid-state and static cultivation methods, respectively. Yam peels favored liberation of 15.3 EU amyloglucosidase in each of the solid-state and the static cultivation regimens. Production of β-amylase on BSG, yam, banana, and plantain peels was not significantly facilitated by static cultivation method (p>0.05), while solid-state cultivation regimen favored amyloglucosidase production on cassava peels and BSG (p<0.05). Optimal production yield of β-amylase and amyloglucosidase were 16.6 and 14.9 EU g −1 on plantain and yam waste media by the mold.

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Section: Effect Of Wastes On Amyloglucosidase Production By Fungal Ismentioning

confidence: 98%

Production of Fungal β-amylase and Amyloglucosidase on Some Nigerian Agricultural Residues

Adeniran

Abiose

Ogunsua

2008

Food Bioprocess Technol

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“…High-positive values of coefficient factor for these variables (11.14 for C 7 , 1.93 for C 2 9 C 7 and 1.09 for C 2 9 C 9 ) also suggest its significance. The R 2 value (coefficient of determination) closer to one denotes better correlation between the observed and predicted values (Xu et al 2008). R 2 for chitinase activity was calculated as 0.8228, which is close to 1 and can explain up to 82.28 % variability of the response.…”

Section: Optimization Of Significant Variables Using Response-surfacementioning

confidence: 99%

Statistical optimization of cold-active chitinase production by mutagenized cells of multi-enzyme producing Bacillus cereus GA6

Roohi

Kuddus

2015

Rend. Fis. Acc. Lincei

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In the present study, four cold-adapted bacterial isolates were screened for multiple-enzyme production at low temperature (15°C). The most potent isolate, Bacillus cereus GA6 (HQ832575), was subjected to mutation by UV radiation to obtain a mutant strain with elevated enzyme production. The mutant strain, designated as CUVGA6, with higher chitinase activity at low temperature was selected for enzyme production optimization using factorial design and response-surface methodology (RSM). Two statistically significant parameters (colloidal chitin and KH 2 PO 4 ) for response were selected (p value = 0.008 and 0.004, respectively) along with pH and temperature and utilized to optimize the process. Central composite design of RSM was used to optimize the levels of key ingredients for the best yield of chitinase. Maximum chitinase production was predicted to be 428.57 U/ml for a 4.4-fold increase in medium containing 2 % colloidal chitin, 6.0 g/L K 2 HPO 4 and pH 9.0 at 25°C when incubated for 7 days in submerged fermentation. ANOVA of CCD suggested that the quadratic interaction effect of K 2 HPO 4 with chitin, temperature and pH has high impact on the production of chitinase (p value = 0.007, 0.002, 0.035, respectively), although its linear effect was not significant as observed. The closeness of optimized values (R 2 = 82.28 %) to experimental values (R 2 = 80.13 %) proved the validity of statistical model. Thus, multi-enzyme producing cold-adapted mutant B. cereus GA6 (CUVGA6) could be exploited for the production of chitinase which is of industrial significance.

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“…Certain yeast and mould strains isolated from various sources, though aerobic, offer considerable potential, since their secreted enzymes are partially adsorbed into the cell wall, thus providing a biomass rich in proteins, nucleotides, and vitamins, as well as a cell extract with hydrolytic activity [5][6][7][8][9]. Random hydrolysis of starch by alpha-amylases produces low-molecular-weight oligosaccharides and, eventually, maltotriose and maltose from amylose and limit dextrins, and maltose and glucose from amylopectin.…”

Section: Introductionmentioning

confidence: 99%

Study of Starch Degradation by Yeasts During Fermentation for Using in Animal Feed

Alonso

Arévalo‐Villena

Úbeda

et al. 2010

Appl Biochem Biotechnol

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Sixteen amylolytic yeasts, drawn from culture collections or isolated from different environments, were used in the present study. Experiments were performed to evaluate the starch degradation due to exocellular activity. The culture media of growth was optimized to find the maximum activity, and some strains produced an extracellular amylolytic enzyme when cultured in presence of starch in the medium. On the other hand, one yeast showed activity in cell extract when cultured under varying conditions in a bench bioreactor; this strain would thus be suitable for use as an animal feed supplement, both as a source of proteins and nucleic acids and in terms of its amylolytic activity.

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Production of α‐amylase by Aspergillus oryzae As 3951 in solid state fermentation using spent brewing grains as substrate

Cited by 31 publications

References 34 publications

Production of Fungal β-amylase and Amyloglucosidase on Some Nigerian Agricultural Residues

Production of Fungal β-amylase and Amyloglucosidase on Some Nigerian Agricultural Residues

Statistical optimization of cold-active chitinase production by mutagenized cells of multi-enzyme producing Bacillus cereus GA6

Study of Starch Degradation by Yeasts During Fermentation for Using in Animal Feed

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