2020
DOI: 10.1016/j.jbiotec.2019.10.021
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Production of α2,6-sialylated and non-fucosylated recombinant alpha-1-antitrypsin in CHO cells

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Cited by 10 publications
(11 citation statements)
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“…The importance of enrichment steps for rapid isolation of cells with targeted glycoprofile was reported previously. [ 22,49,50 ] Unexpectedly, wider histogram peaks were observed in Fut8 enriched clones with the lowest FI (see Figure 2A and Figure S3a). While the reason for this is unclear, it suggests potential epigenetic variability around the Fut8 gene in individual cells expressing KRAB‐SadCas9.…”
Section: Discussionmentioning
confidence: 89%
“…The importance of enrichment steps for rapid isolation of cells with targeted glycoprofile was reported previously. [ 22,49,50 ] Unexpectedly, wider histogram peaks were observed in Fut8 enriched clones with the lowest FI (see Figure 2A and Figure S3a). While the reason for this is unclear, it suggests potential epigenetic variability around the Fut8 gene in individual cells expressing KRAB‐SadCas9.…”
Section: Discussionmentioning
confidence: 89%
“…In addition to cell engineering strategies, various additives supplemented to the culture medium can enhance the sialic acid biosynthesis pathway and thus increase the sialylation of glycoproteins during their production. Supplementation of culture medium with ManNAc, a precursor of sialic acid, was previously shown to increase sialylation of recombinant glycoproteins (Baker et al, 2001; Gu & Wang, 1998; Lalonde et al, 2019). We thus investigated whether the addition of 20 mM ManNAc during production of the mutein pool had a positive effect on A1AT sialylation.…”
Section: Resultsmentioning
confidence: 99%
“…Apart from adequate expression levels, appropriate glycosylation is another critical attribute for recombinant proteins intended for therapeutic use. Several studies have reported that the antiprotease activity of A1AT is not influenced by its N ‐glycosylation pattern (Amann et al, 2019; Lalonde et al, 2019; K. J. Lee et al, 2013). In agreement with these studies, we also found that the inhibitory activity of both rA1ATs towards neutrophil elastase was comparable to that of Prolastin‐C.…”
Section: Discussionmentioning
confidence: 99%
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