Production of ε-polylysine in an airlift bioreactor (ABR)

Kahar, Prihardi; Kobayashi, Kengo; Iwata, Takehiko; Hiraki, Jun; KOJIMA, MAMI; Okabe, Mitsuyasu

doi:10.1016/s1389-1723(02)80028-7

Cited by 37 publications

(10 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[18][19][20] EPL has been shown to be practically non-toxic in an acute oral toxicity study on rats, with no mortality up to 5 g/kg, and was not mutagenic in bacterial reversion assays. 21) It also lacked toxicological effects in subchronic and chronic feeding bioassays using relatively high concentrations in the diet of up to 50,000 ppm.…”

Section: Discussionmentioning

confidence: 99%

Inhibition by ε-Polylysine of Fat Digestion in the Stomach and Intestine of Rats

Tsujita

Takaku

2009

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

In vitro, the inhibition by epsilon-polylysine depends on how the substrate is presented to the lipase. We therefore examined whether epsilon-polylysine can interact with the lipid emulsion and prevent lipase activity in digestive organs. To confirm lipase inhibition by epsilon-polylysine, a (14)C-trioleoylglycerol emulsion with or without epsilon-polylysine was orally administered to rats, and the radioactive lipid distribution determined at regular intervals. The radioactive plasma lipid was decreased, and radioactive fecal lipid was increased by the administration of epsilon-polylysine. The peak of radioactive lipids in the intestine was delayed by the administration of epsilon-polylysine. We used 20-week-old rats as a model for the middle-aged and elderly to test the effect of epsilon-polylysine on the body weight increase. epsilon-Polylysine significantly prevented any elevation in body weight and weight of the liver and epididymal adipose tissues. These data show that epsilon-polylysine inhibited the lipase activity in the digestive organ and had an anti-obesity function in the middle-aged rats.

show abstract

Section: Discussionmentioning

confidence: 99%

Inhibition by ε-Polylysine of Fat Digestion in the Stomach and Intestine of Rats

Tsujita

Takaku

2009

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

show abstract

“…According to Kahar et al [15], mycelium growth and morphology, and formation of products are related to the type of bioreactor used. For A. oryzae IPT301, apparently, the expected shearing eVect of the STR turbines is not so critical.…”

Section: Cultivation In Airlift Bioreactor (Alb)mentioning

confidence: 99%

Production of xylanolytic enzymes by Aspergillus terricola in stirred tank and airlift tower loop bioreactors

Michelin

Polizeli

Silva

et al. 2011

J Ind Microbiol Biotechnol

View full text Add to dashboard Cite

Fungi producing high xylanase levels have attracted considerable attention because of their potential industrial applications. Batch cultivations of Aspergillus terricola fungus were evaluated in stirred tank and airlift bioreactors, by using wheat bran particles suspended in the cultivation medium as substrate for xylanase and β-xylosidase production. In the stirred tank bioreactor, in physical conditions of 30°C, 300 rpm, and aeration of 1 vvm (1 l min⁻¹), with direct inoculation of fungal spores, 7,475 U l⁻¹ xylanase was obtained after 36 h of operation, remaining constant after 24 h. In the absence of air injection in the stirred tank reactor, limited xylanase production was observed (final concentration 740 U l⁻¹). When the fermentation process was realized in the airlift bioreactor, xylanase production was higher than that observed in the stirred tank bioreactor, being 9,265 U l⁻¹ at 0.07 vvm (0.4 l min⁻¹) and 12,845 U l⁻¹ at 0.17 vvm (1 l min⁻¹) aeration rate.

show abstract

“…However, researchers have not given up on designing new bioreactors for ε-PL production. As early as 2002, an airlift bioreactor was evaluated for ε-PL production, which promised low production cost, energy consumption, and downstream processing (Kahar et al 2002). Because ɛ-PL is a cationic polymer, the anionic resin could be used to absorb the accumulated ɛ-PL.…”

Section: Fermentative Production Of ɛ-Pl With Waste Feedstockmentioning

confidence: 99%

Recent advances in the biotechnological production of microbial poly(ɛ-l-lysine) and understanding of its biosynthetic mechanism

Feng

et al. 2016

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Poly(ɛ-L-lysine) (ɛ-PL) is an unusual biopolymer composed of L-lysine connected between α-carboxyl and ɛ-amino groups. It has been used as a preservative in food and cosmetics industries, drug carrier in medicines, and gene carrier in gene therapy. Modern biotechnology has significantly improved the synthetic efficiency of this novel homopoly(amino acid) on an industrial scale and has expanded its industrial applications. In the latest years, studies have focused on the biotechnological production and understanding the biosynthetic mechanism of microbial ɛ-PL. Herein, this review focuses on the current trends and future perspectives of microbial ɛ-PL. Information on the screening of ɛ-PL-producing strains, fermentative production of ɛ-PL, breeding of high-ɛ-PL-producing strains, genomic data of ɛ-PL-producing strains, biosynthetic mechanism of microbial ɛ-PL, and the control of molecular weight of microbial ɛ-PL is included. This review will contribute to the development of this novel homopoly(amino acid) and serve as a basis of studies on other biopolymers.

show abstract

Production of ε-polylysine in an airlift bioreactor (ABR)

Cited by 37 publications

References 11 publications

Inhibition by ε-Polylysine of Fat Digestion in the Stomach and Intestine of Rats

Inhibition by ε-Polylysine of Fat Digestion in the Stomach and Intestine of Rats

Production of xylanolytic enzymes by Aspergillus terricola in stirred tank and airlift tower loop bioreactors

Recent advances in the biotechnological production of microbial poly(ɛ-l-lysine) and understanding of its biosynthetic mechanism

Contact Info

Product

Resources

About