Production, physicochemical characteristics, and in vitro biological activities of polysaccharides obtained from fresh bitter gourd (Momordica charantia L.) via room temperature extraction techniques

Yan, Jun; Yu, Yun-Bo; Wang, Chun; Cai, Wu-Dan; Wu, Liqing; Yang, Yan; Zhang, He-Nan

doi:10.1016/j.foodchem.2020.127798

Cited by 70 publications

(38 citation statements)

References 35 publications

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“…A previously published protocol with slight modifications [15], was referred for evaluating DPPH radical scavenging efficacy of BSPs. BSPs samples (1 mL) at different concentrations were added into 1 mL DPPH solution dissolved in dehydrated alcohol (0.004%, w/v), then shaken and incubated for 30 min in a dark place.…”

Section: Antioxidant Activities Dpph Radical Scavenging Activitymentioning

confidence: 99%

Characterization of polysaccharides from different species of brown seaweed using saccharide mapping and chromatographic analysis

et al. 2021

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Brown seaweed polysaccharides (BSPs) are one of the primary active components from brown seaweed that has a range of pharmaceutical and biomedical applications. However, the quality control of BSPs is a challenge due to their complicated structure and macromolecule. In this study, saccharide mapping based on high-performance liquid chromatography (HPLC), multi-angle laser light scattering, viscometer, and refractive index detector (HPSEC-MALLS-Vis-RID), and Fourier transform infrared (FT-IR) were used to discriminate the polysaccharides from nine different species of brown algae (BA1-9). The results showed that BSPs were composed of β-D-glucans and β-1,3−1,4-glucan linkages. The molecular weight, radius of gyration, and intrinsic viscosity of BSPs were ranging from 1.718 × 105 Da to 6.630 × 105 Da, 30.2 nm to 51.5 nm, and 360.99 mL/g to 865.52 mL/g, respectively. Moreover, α values of BSPs were in the range of 0.635 to 0.971, which indicated a rigid rod chain conformation. The antioxidant activities of BSPs exhibited substantial radical scavenging activities against DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2, 2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) radicals, which indicated that the use of BSPs might be a potential approach for antioxidant supplements. Thus, this study gives insights about the structure-function relationship of BSPs, which will be beneficial to improve the quality of polysaccharides derived from marine algae.

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Section: Antioxidant Activities Dpph Radical Scavenging Activitymentioning

confidence: 99%

Characterization of polysaccharides from different species of brown seaweed using saccharide mapping and chromatographic analysis

et al. 2021

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“…Previous studies have found that plant ingredients such as ursolic acid [33], maidenhair fern [34], and Poria cocos [35] have protective effects in delaying the progression of PD and reducing symptoms of PD. MCPs account for about 6% of bitter melon powder, which are heteropolysaccharides, composed of galactose (Gal), glucose (Glu), arabinose (Ara), rhamnose (Rha), and mannose (Man) [36]. Tan and Gan [37] have reported that an acidic 11 International Journal of Polymer Science branched heteropolysaccharide isolated from bitter melon is mainly composed of Man, galacturonic acid (GalA), Rha, Glu, Gal, xylose (Xyl), and Ara, which has antioxidant and inhibitory properties.…”

Section: Discussionmentioning

confidence: 99%

Momordica Charantia Polysaccharides Attenuates MPP+-Induced Injury in Parkinson’s Disease Mice and Cell Models by Regulating TLR4/MyD88/NF-κB Pathway

Guo

Zhou

Zhang

et al. 2021

International Journal of Polymer Science

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Objective. To investigate the potential role of Momordica charantia polysaccharides (MCPs) in Parkinson’s disease (PD) and reveal the molecular mechanism of its function. Method. 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and 1-methyl-4-phenylpyridinium (1-methyl-4-phenylpyridinium, MPP+) were used to establish PD mice and cell models. The mice and cells were divided into 4 groups: Control group, Control+MCPs group, PD group, and PD+MCPs group. Pole climbing experiment and Rotarod experiment were used to observe the coordination ability of mice. High-performance liquid chromatography and enzyme-linked immunosorbent assay (ELISA) were used to determine neurotransmitters and metabolites, inflammatory factors TNF-α and IL-1β, oxidative stress-related markers SOD, MDA, and GSH in striatum tissues. Western blot was used to determine the protein levels of tyrosine hydroxylase (TH), oxidative stress-related protein Cytochrome C (Cytochrome C), and apoptosis-related proteins Bcl-2, Bax, and cleaved Caspase-3 in tissues and cells. Moreover, flow cytometry, PI staining, and fluorescence were used to observe cell apoptosis. Finally, the activation effect of MCPs on TLR4/MyD88/NF-κB signaling pathway was observed and verified. Results. Compared with the Control group, MPTP treatment can induce brain damage in mice (all P < 0.05 ), change the metabolic state of neurotransmitters (all P < 0.05 ), induce inflammation (all P < 0.05 ), and induce apoptosis and the occurrence of oxidation reaction (all P < 0.05 ); however, MCPs treatment can significantly reverse the above changes (all P < 0.05 ). In cell models, studies have found that MCPs can play a protective role by regulating the activation state of TLR4/MyD88/NF-κB pathway. Conclusion. This study found that the application of MCPs therapy can play anti-inflammatory, antioxidative stress, and antiapoptotic effects in PD by regulating the activation of the TLR4/MyD88/NF-κB pathway.

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“…The reducing power of BGP was characterized according to a previous method reported by Yan et al [ 12 ]. The molecular weight of the BGP was measured by gel permeation chromatography (GPC, Kyoto, Shimadzu, Japan), the gel column was filled with dextran and eluted with ultrapure water, and the elution curve and the relative molecular weight of the polysaccharide were measured.…”

Section: Methodsmentioning

confidence: 99%

Biocompatible Platinum Nanoclusters Prepared Using Bitter Gourd Polysaccharide for Colorimetric Detection of Ascorbic Acid

Zhao

Zhang

et al. 2021

Biomolecules

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Ascorbic acid is an organic compound with antioxidant properties that can protect the human body from the threat of free radicals. Therefore, it is important to detect the existence and measure the concentration of ascorbic acid to regulate its content in the human body. In this work, we prepared bitter gourd polysaccharide (BGP)-stabilized platinum nanoclusters (Pt-BGP NCs) by reacting BGP with K2PtCl4. Pt-BGP NCs and catalyzed the decomposition of H2O2 to generate •OH radicals, which could oxidize TMB to form oxidized TMB (oxTMB), indicating their peroxidase-like properties. The kinetics followed the Michaelis–Menten equation. Furthermore, the colorimetric detection of ascorbic acid using Pt-BGP NCs showed high selectivity and a low detection limit of 0.191 μM. The accuracy of real sample detection using Pt-BGP NCs was as high as 98.9%. More importantly, Pt-BGP NCs had high cell biocompatibility and extremely low hemolysis rate due to the component of BGP. In summary, the prepared Pt-BGP NCs with reductive activity and good biocompatibility have good application prospects in colorimetric detection of ascorbic acid.

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Production, physicochemical characteristics, and in vitro biological activities of polysaccharides obtained from fresh bitter gourd (Momordica charantia L.) via room temperature extraction techniques

Cited by 70 publications

References 35 publications

Characterization of polysaccharides from different species of brown seaweed using saccharide mapping and chromatographic analysis

Characterization of polysaccharides from different species of brown seaweed using saccharide mapping and chromatographic analysis

Momordica Charantia Polysaccharides Attenuates MPP+-Induced Injury in Parkinson’s Disease Mice and Cell Models by Regulating TLR4/MyD88/NF-κB Pathway

Biocompatible Platinum Nanoclusters Prepared Using Bitter Gourd Polysaccharide for Colorimetric Detection of Ascorbic Acid

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